Robinin
Based on 7 publication(s) in Google Scholar
Robinin is a flavonoid that can be extracted from the leaves of purple cowpea, inhibiting TGF-β, TLR4/NF-κB and TLR2-PI3k-AKT signaling pathways. Robinin exerts anti-inflammatory and anti-tumor effects. The combination of Robinin and Methotrexate (HY-14519) reduces inflammation in experimental arthritis, Robinin can decrease the Doxorubicin (HY-15142A) induced cardiac toxicity effect.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.96%
- No. CAS: 301-19-9
- Fòrmula: C33H40O19
- Peso molecular:740.66
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Robinin
More- Metabolism. 2026 Jul:180:156622. [Abstract]
- Food Chem. 2025 Dec 30:497:146992. [Abstract]
- Food Chem. 2025 Oct 15:489:144992. [Abstract]
- Ind Crops Prod. 2026 May 8;246:123392.
- Toxicol Appl Pharmacol. 2024 May 20:487:116976. [Abstract]
- Cell Biochem Biophys. 2025 Mar;83(1):647-656. [Abstract]
- Research Square Preprint. 2023 Dec 25.
Customer Validation & Images
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Cell Proliferation/Viability Assay
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Apoptosis Analysis
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WB
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IF
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ELISA
Actividad biológica
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TLR2 |
TLR4 |
Robinin (1-10 μg/ml, 24 h) shows no obvious cytotoxicity in hPBMCs cells[1].
Robinin (6 μg/mL, 24 h) inhibits the expression of MCP1, TNF-α, IL-6 and ICAM-1 proteins and the mRNA levels of TLR2 and TLR4 in hPBMCs cells, and had anti-inflammatory effects [1].
Robinin (1 μM, 24 h) inhibits the proliferation and migration of Mia-PACA2 and PANC-1 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:hPBMCs
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Concentration:6 μg/mL
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Incubation Time:24 h
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Result:Inhibited the upregulated expression of MCP 1, TNF-α, IL-6 and ICAM-1 with anti-inflammatory properties.
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Cell Line:hPBMCs
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Concentration:1 μg/mL, 2 μg/mL,4 μg/mL,6 μg/mL,8 μg/mL,10 μg/mL
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Incubation Time:24 h
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Result:Concentration from 1 μg/ml-10 μg/ml on hPBMCs had no obvious cytotoxicity.
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Cell Line:hPBMCs
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Concentration:1 μg/mL, 2 μg/mL,4 μg/mL,6 μg/mL,8 μg/mL,10 μg/mL
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Incubation Time:24 h
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Result:Inhibited the upregulated expression of TLR2 and TLR4.
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Cell Line:Mia-PACA2 and PANC-1
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Concentration:1 μM
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Incubation Time:24 h
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Result:Inhibited cell proliferation.
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Cell Line:Mia-PACA2 and PANC-1
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Concentration:1 μM
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Incubation Time:24 h
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Result:Decreased the cell migration area
Robinin (50 mg/kg, Oral gavage, 10 days) can modulate the cardioprotective effect of the TGF-β1 signaling pathway on Adriamycin-induced cardiotoxicity[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Adjuvant arthritis mice model[3]
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Dosage:50 mg/kg Robinin and 0.3 mg/kg MTX twice a week
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Administration:Oral gavage (p.o.)
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Result:Had anti-inflammatory and anti-arthritic action.
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Animal Model:Sprague Dawley rats model[4]
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Dosage:50 mg/kg Robinin for 10 days
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Administration:Oral gavage (p.o.)
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Result:Reduced the cardiotoxic effects of Doxorubicin-induced
Chemical Information
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No. CAS 301-19-9
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Appearance Solid
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Peso molecular 740.66
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Fòrmula C33H40O19
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Color White to yellow
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SMILES
O=C1C(O[C@H]2[C@@H]([C@H]([C@H]([C@@H](CO[C@H]3[C@@H]([C@@H]([C@H]([C@H](C)O3)O)O)O)O2)O)O)O)=C(C4=CC=C(O)C=C4)OC5=CC(O[C@H]6[C@@H]([C@@H]([C@H]([C@H](C)O6)O)O)O)=CC(O)=C15
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (7)
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Journal Impact Factor
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Most Recent
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Metabolism
2026 Jul:180:156622. PMID: 41997496 -
Food Chem
Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]2025 Dec 30:497:146992. PMID: 41285060 -
Food Chem
Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]2025 Oct 15:489:144992. PMID: 40466530 -
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Toxicol Appl Pharmacol
RGS2 attenuates alveolar macrophage damage by inhibiting the Gq/11-Ca2+ pathway during cowshed PM2.5 exposure, and aberrant RGS2 expression is associated with TLR2/4 activation. [Abstract]2024 May 20:487:116976. PMID: 38777097 -
Cell Biochem Biophys
2025 Mar;83(1):647-656. PMID: 39673685
Robinin purchased from MedChemExpress. Usage Cited in: Cell Biochem Biophys. 2025 Mar;83(1):647-656. [Abstract]
CCK-8 showed the cell viability in the isolated mouse chondrocytes under the treatment of IL-1β and Robinin (2, 4, 6, 8, 10, 12 μg/mL).
Robinin purchased from MedChemExpress. Usage Cited in: Cell Biochem Biophys. 2025 Mar;83(1):647-656. [Abstract]
Flow cytometry showed the cell apoptosis in the isolated mouse chondrocytes under the treatment of IL-1β and Robinin (10 μg/mL).
Robinin purchased from MedChemExpress. Usage Cited in: Cell Biochem Biophys. 2025 Mar;83(1):647-656. [Abstract]
Western blotting evaluated the levels of apoptosis-related proteins in chondrocytes treated with IL-1β and Robinin (10 μg/mL).
Robinin purchased from MedChemExpress. Usage Cited in: Cell Biochem Biophys. 2025 Mar;83(1):647-656. [Abstract]
Immunofluorescence staining in chondrocytes treated with IL-1β and Robinin (10 μg/mL).
Robinin purchased from MedChemExpress. Usage Cited in: Cell Biochem Biophys. 2025 Mar;83(1):647-656. [Abstract]
ELISA showed the concentrations of inflammatory markers in the supernatant of the chondrocytes treated with IL-1β and Robinin (10 μg/mL).
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Solvente y solubilidad
DMSO : 250 mg/mL (337.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (2.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (2.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (288 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Janeesh PA, et al. Robinin modulates TLR/NF-κB signaling pathway in oxidized LDL induced human peripheral blood mononuclear cells. Int Immunopharmacol. 2014 Jan;18(1):191-7. [Content Brief]
[3]. Tsiklauri L, et al. Bioflavonoid Robinin from Astragalus falcatus Lam. Mildly Improves the Effect of Metothrexate in Rats with Adjuvant Arthritis. Nutrients. 2021 Apr 13;13(4):1268. [Content Brief]
[4]. Janeesh PA, et al. Robinin modulates doxorubicin-induced cardiac apoptosis by TGF-β1 signaling pathway in Sprague Dawley rats. Biomed Pharmacother. 2014 Oct;68(8):989-98. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.3501 mL | 6.7507 mL | 13.5015 mL | 33.7537 mL |
| 5 mM | 0.2700 mL | 1.3501 mL | 2.7003 mL | 6.7507 mL | |
| 10 mM | 0.1350 mL | 0.6751 mL | 1.3501 mL | 3.3754 mL | |
| 15 mM | 0.0900 mL | 0.4500 mL | 0.9001 mL | 2.2502 mL | |
| 20 mM | 0.0675 mL | 0.3375 mL | 0.6751 mL | 1.6877 mL | |
| 25 mM | 0.0540 mL | 0.2700 mL | 0.5401 mL | 1.3501 mL | |
| 30 mM | 0.0450 mL | 0.2250 mL | 0.4500 mL | 1.1251 mL | |
| 40 mM | 0.0338 mL | 0.1688 mL | 0.3375 mL | 0.8438 mL | |
| 50 mM | 0.0270 mL | 0.1350 mL | 0.2700 mL | 0.6751 mL | |
| 60 mM | 0.0225 mL | 0.1125 mL | 0.2250 mL | 0.5626 mL | |
| 80 mM | 0.0169 mL | 0.0844 mL | 0.1688 mL | 0.4219 mL | |
| 100 mM | 0.0135 mL | 0.0675 mL | 0.1350 mL | 0.3375 mL |
Robinin Related Classifications
HY-N1346 Related Classifications
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