GSK2618960 

GSK2618960 is a human monoclonal antibody (mAb) targeting IL-7Ra/CD127. GSK2618960 inhibits IL-7-induced STAT5 phosphorylation. GSK2618960 enhances CD4 T cell proliferation response and increases CD83, CD86, and CD209 expression in PBMCs. GSK2618960 can be used for the research of autoimmune and allergic inflammatory diseases. Recommended isotype control: Human IgG1 kappa, Isotype Control (HY-P99001)^[1][2].

Human IgG1 kappa

Human IgG1 kappa, Isotype Control

Human

GSK2618960 (10-100 μg/mL; 10 h) potently induces dose-dependent CD4⁺ T cell proliferation in PBMCs^[2].
GSK2618960 binds specifically to monocyte-derived DCs, but not to uncultured primary DCs, NK cells, or B cells from fresh PBMCs^[2].
GSK2618960 (50 μg/mL; 7 days) enhances the differentiation of DCs and upregulates their activation marker (CD83, CD86 and CD209) expression in PBMCs^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3575  [NCBI]

P16871

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

144.93 kDa

Liquid

Colorless to light yellow

[GSK2618960]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Flow Cytometry analysis of K562 cells labelling CD127 (red) with GSK2618960 (HY-P991424). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

• [1]. Ellis J, et al. Anti-IL-7 receptor α monoclonal antibody (GSK2618960) in healthy subjects - a randomized, double-blind, placebo-controlled study. Br J Clin Pharmacol. 2019;85(2):304-315.  [Content Brief]

  [2]. Liao K, et al. Characterization of the robust humoral immune response to GSK2618960, a humanized anti-IL-7 receptor monoclonal antibody, observed in healthy subjects in a Phase 1 study. PLoS One. 2021;16(3):e0249049. Published 2021 Mar 23.  [Content Brief]