HDAC6-IN-10
HDAC6-IN-10 is a highly selective HDAC6 inhibitor with the IC50 of 0.73 nM. HDAC6-IN-10 has 144~10941-fold selectivity over other HDAC isoforms. HDAC6-IN-10 shows anti-proliferative activities against multiple myeloma cells.
For research use only. We do not sell to patients.
- CAS No.: 2408286-73-5
- Formula: C21H20N4O4
- Molecular Weight:392.41
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
HDAC6 0.73 nM (IC50) |
HDAC10 105 nM (IC50) |
HDAC8 513 nM (IC50) |
HDAC7 752 nM (IC50) |
HDAC11 1800 nM (IC50) |
HDAC9 2560 nM (IC50) |
HDAC5 4370 nM (IC50) |
HDAC4 5620 nM (IC50) |
HDAC1 8020 nM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| RPMI-8226 | IC50 |
33.183 μM
Compound: 21b
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Antiproliferative activity against human RPMI-8226 cells assessed as inhibition of cell growth incubated for 72 hrs by alamar blue assay
Antiproliferative activity against human RPMI-8226 cells assessed as inhibition of cell growth incubated for 72 hrs by alamar blue assay
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[PMID: 31865013] |
| U-266 | IC50 |
43.233 μM
Compound: 21b
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Antiproliferative activity against human U-266 cells assessed as inhibition of cell growth incubated for 72 hrs by alamar blue assay
Antiproliferative activity against human U-266 cells assessed as inhibition of cell growth incubated for 72 hrs by alamar blue assay
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[PMID: 31865013] |
In Vitro
HDAC6-IN-10 (Compound 21b) (0.1-10 μM; 24 h) treatment shows highly selective inhibition against HDAC6[1]. HDAC6-IN-10 (Compound 21b) (0-100 μM; 72 h) treatment shows anti-proliferative activities against two multiple myeloma cells RPMI-8226 and U266[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HCT-116
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Concentration:0.1, 1 and 10 μM
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Incubation Time:24 hours
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Result:Showed a dose-dependent increase in the level of Ac-tubulin.
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Cell Line:RPMI-8226 and U266 cells
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Concentration:0-100 μM
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Incubation Time:72 hours
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Result:Showed antiproliferative activities against RPMI-8226 and U266 with the IC50s of 33.183 μM and 43.233 μM, respectively.
Chemical Information
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CAS No. 2408286-73-5
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Molecular Weight 392.41
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Formula C21H20N4O4
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SMILES
O=C(NO)C1=CC=C(CN2C([C@@H](CC3=CNC4=C3C=CC=C4)NC(C2)=O)=O)C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
HDAC6-IN-10 Related Classifications
HY-150595 Related Classifications
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