Cat. No.: HY-121389

Hexadecanamide

(Synonyms: Palmitamide)

Customer Review

Based on 1 Customer Validation

Hexadecanamide (Palmitamide) is a fatty acid amide that has orally active anti-allergic, antioxidant, and neuroprotective effects. Hexadecanamide exerts protective effects in Staphylococcus aureus- and SARA-induced mastitis. Hexadecanamide suppresses S. aureus-induced activation of the NF-κB pathway and improves blood-milk barrier integrity. Hexadecanamide activates PPARα. Hexadecanamide enhances sperm motility in vitro. Hexadecanamide can be studied in research for mastitis and asthenozoospermia.

For research use only. We do not sell to patients.

Purity: 99.64%
CAS No.: 629-54-9
Formula: C₁₆H₃₃NO
Molecular Weight:255.44

Storage:

Store at room temperature 3 years.

In solvent -80°C, 2 years , -20°C, 1 year

Hexadecanamide Chemical Structure

CAS No.: 629-54-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

PPARα

All NF-κB Isoforms :

All PPAR Isoforms :

In Vitro

Hexadecanamide (Compound HEX) (5 μM, 2 h) limits the activation of mammary NF-κB via activating the PPARα-SIRT1 axis in MMECs^[1].
Hexadecanamide (10 nM, 0-72 h) exerts a significant enhancing effect on sperm motility^[2].
Hexadecanamide (0-1.5 nM, 72 h) results in a significant increase in the protein levels of both PAOX and CA2 in sperm cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Hexadecanamide (Compound HEX) (1.25-5 mg/kg. p.o., 15 d) reduces mammary S. aureus loads and attenuates mammary damage in a dose-dependent manner in S. aureus infected mice^[1].
Hexadecanamide (5 mg/kg, p.o., 10 d) effectively reduces colonic inflammation in SARA mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

S. aureus infected male and female BALB/c mice (21-25 g, 6-8 w)^[1]
Dosage:

1.25, 2.5, 5 mg/kg
Administration:

Oral gavage (p.o.), 15 d
Result:

Reduced inflammatory cell infiltration and structure destruction.
Decreased inflammatory markers (MPO activity, TNF-α, IL-1β) dose-dependently.
Reversed the decrease in the TJs proteins, ZO-1, Occludin, and Claudin-3 caused by S. aureus.
Repaired the blood-milk barrier injury induced by S. aureus.

Animal Model:

BALB/c mice model with S-RMT-induced mucosal barrier injury^[1]
Dosage:

5 mg/kg
Administration:

Oral gavage (p.o.), 10 d
Result:

Increased mucin-2 expression and improved S-RMT-induced mucosal barrier injury in S-RMT mice.
Distinctly mitigated the upregulation of inflammatory markers caused by S-RMT.
Reversed the SARA aggravated S. aureus-induced mastitis.

Chemical Information

CAS No. 629-54-9
Appearance Solid
Molecular Weight 255.44
Formula C₁₆H₃₃NO
Color White to off-white
SMILES

NC(CCCCCCCCCCCCCCC)=O
Synonyms

Palmitamide
Structure Classification
- Alkaloids
- Other Alkaloids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (19.57 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.9148 mL

19.5741 mL

39.1481 mL

5 mM

0.7830 mL

3.9148 mL

7.8296 mL

10 mM

0.3915 mL

1.9574 mL

3.9148 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 0.5 mg/mL (1.96 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline

Solubility: 5 mg/mL (19.57 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (269 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Bao L, et al. Hexadecanamide alleviates Staphylococcus aureus-induced mastitis in mice by inhibiting inflammatory responses and restoring blood-milk barrier integrity. PLoS Pathog. 2023 Nov 10;19(11):e1011764. [Content Brief]

[2]. Han B, et al. Changes in seminal plasma microecological dynamics and the mechanistic impact of core metabolite hexadecanamide in asthenozoospermia patients. Imeta. 2024 Jan 25;3(2):e166. https://pubmed.ncbi.nlm.nih.gov/38882497/ [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.9148 mL	19.5741 mL	39.1481 mL	97.8703 mL
	5 mM	0.7830 mL	3.9148 mL	7.8296 mL	19.5741 mL
	10 mM	0.3915 mL	1.9574 mL	3.9148 mL	9.7870 mL
	15 mM	0.2610 mL	1.3049 mL	2.6099 mL	6.5247 mL

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Hexadecanamide

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All NF-κB Isoforms

All PPAR Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Hexadecanamide Related Classifications

HY-121389 Related Classifications