Serplulimab
Based on 1 Customer Validation
Serplulimab (HLX 10) is a humanized anti-PD-1 monoclonal antibody. Serplulimab can inhibit tumor growth, regulate the tumor microenvironment, and has anti-tumor activity. Serplulimab can be used in the research of cancer such as lung cancer and colon cancer.
For research use only. We do not sell to patients.
- Purity: 99.30%
- CAS No.: 2231029-82-4
- Molecular Weight:144.18 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Human IgG4 kappa
Human
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p-STAT3 |
Akt1 |
Akt2 |
Akt3 |
Serplulimab (10 μg/mL; 0-16 h) can increase the phosphorylation levels of AKT1/2/3 (T308), AKT1/2/3 (S473), and STAT3 (Y705) in Jurkat-PD-1 cells in response to PD-1 signaling blockade[1].
Serplulimab (0-1000 ng/mL; 2 days) can enhance the production of IFNγ in T cells, increase the cytotoxicity against SGC7901, and reverse the inhibitory effect of SGC7901 on T-cell effector functions in the co-culture system of CD8+ T cells and SGC7901 gastric cancer cells. The effect is even better when combined with HLX53[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c-hPD-1/hTIGIT dKI HuGEMM mice engrafted with CT26
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Dosage:/
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Administration:Intraperitoneal injection; twice a week; until the end of the experiment
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Result:Showed moderate anti-tumor efficacy. When combined with HLX53, it exerted a notably enhanced inhibition on tumor growth, with a tumor growth inhibition (TGI) of 66.09%.
Enhanced survival of mice.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Human IgG4 kappa
ELISA, FACS, Functional assay
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Flow cytometric analysis of 1X106 10 ng/mL PMA and 1 μg/mL Ionomycin co-stimulated Jurkat cells (24h) with Serplulimab (HY-P99118, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG4 kappa (HY-P99003, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
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CAS No. 2231029-82-4
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Appearance Liquid
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Molecular Weight 144.18 kDa
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Color Colorless to light yellow
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SMILES
[Serplulimab]
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Synonyms
HLX 10
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (260 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Zhang Y, et al. Insights into the mechanisms of serplulimab: a distinctive anti-PD-1 monoclonal antibody, in combination with a TIGIT or LAG3 inhibitor in preclinical tumor immunotherapy studies. MAbs. 2024 Jan-Dec;16(1):2419838. [Content Brief]
[2]. Lee A. Serplulimab: First Approval. Drugs. 2022 Jul;82(10):1137-1141. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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