MS645
Based on 1 publication(s) in Google Scholar
MS645 is a bivalent BET bromodomains (BrD) inhibitor with a Ki of 18.4 nM for BRD4-BD1/BD2. MS645 spatially constrains bivalent inhibition of BRD4 BrDs resulting in a sustained repression of BRD4 transcriptional activity in solid-tumor cells.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 2250091-96-2
- Formula: C48H54Cl2N10O2S2
- Molecular Weight:938.04
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Storage:
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) MS645
More
Biological Activity
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BRD4(BD1BD2) 18.4 nM (Ki) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| BT-549 | IC50 |
6.8 nM
Compound: 95; MS645
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Anticancer activity against human BT-549 cells assessed as cell growth inhibition incubated for 4 to 10 days by MTT assay
Anticancer activity against human BT-549 cells assessed as cell growth inhibition incubated for 4 to 10 days by MTT assay
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[PMID: 33650861] |
| HCC1806 | IC50 |
19 nM
Compound: 95; MS645
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Anticancer activity against human HCC1806 cells assessed as cell growth inhibition incubated for 4 to 10 days by MTT assay
Anticancer activity against human HCC1806 cells assessed as cell growth inhibition incubated for 4 to 10 days by MTT assay
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[PMID: 33650861] |
| HCC38 | IC50 |
15 nM
Compound: 95; MS645
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Anticancer activity against human HCC38 cells assessed as cell growth inhibition incubated for 4 to 10 days by MTT assay
Anticancer activity against human HCC38 cells assessed as cell growth inhibition incubated for 4 to 10 days by MTT assay
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[PMID: 33650861] |
| Hs-578T | IC50 |
4.1 nM
Compound: 95; MS645
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Anticancer activity against human Hs-578T cells assessed as cell growth inhibition incubated for 4 to 10 days by MTT assay
Anticancer activity against human Hs-578T cells assessed as cell growth inhibition incubated for 4 to 10 days by MTT assay
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[PMID: 33650861] |
| MDA-MB-231 | IC50 |
10.3 nM
Compound: 95; MS645
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Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 4 to 10 days by MTT assay
Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 4 to 10 days by MTT assay
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[PMID: 33650861] |
MS645 has cell growth inhibitory effects on noncancer cell lines of mouse macrophage RAW cells and nontumorigenic breast epithelial MCF10A with IC50s of 4.1 nM, 6.8 nM, 7.9 nM for triple-negative breast cancer (TNBC) cell lines HS5878T, BT549, and MCF 10A[1].
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MS645 (15, 30, 60 nM) results in a dramatic reduction of c-Myc expression and an increase of p21, a tumor suppressor and cell-cycle inhibitor in HCC1806 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2250091-96-2
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Appearance Solid
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Molecular Weight 938.04
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Formula C48H54Cl2N10O2S2
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Color Light yellow to yellow
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SMILES
O=C(NCCCCCCCCCCNC(C[C@H]1C2=NN=C(C)N2C3=C(C(C)=C(C)S3)C(C4=CC=C(Cl)C=C4)=N1)=O)C[C@H]5C6=NN=C(C)N6C7=C(C(C)=C(C)S7)C(C8=CC=C(Cl)C=C8)=N5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (1)
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Journal Impact Factor
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Most Recent
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Cell
2025 Oct 2;188(20):5632-5652.e25. PMID: 40680734
Solvent & Solubility
DMSO : 200 mg/mL (213.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (5.33 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.0661 mL | 5.3303 mL | 10.6605 mL | 26.6513 mL |
| 5 mM | 0.2132 mL | 1.0661 mL | 2.1321 mL | 5.3303 mL | |
| 10 mM | 0.1066 mL | 0.5330 mL | 1.0661 mL | 2.6651 mL | |
| 15 mM | 0.0711 mL | 0.3554 mL | 0.7107 mL | 1.7768 mL | |
| 20 mM | 0.0533 mL | 0.2665 mL | 0.5330 mL | 1.3326 mL | |
| 25 mM | 0.0426 mL | 0.2132 mL | 0.4264 mL | 1.0661 mL | |
| 30 mM | 0.0355 mL | 0.1777 mL | 0.3554 mL | 0.8884 mL | |
| 40 mM | 0.0267 mL | 0.1333 mL | 0.2665 mL | 0.6663 mL | |
| 50 mM | 0.0213 mL | 0.1066 mL | 0.2132 mL | 0.5330 mL | |
| 60 mM | 0.0178 mL | 0.0888 mL | 0.1777 mL | 0.4442 mL | |
| 80 mM | 0.0133 mL | 0.0666 mL | 0.1333 mL | 0.3331 mL | |
| 100 mM | 0.0107 mL | 0.0533 mL | 0.1066 mL | 0.2665 mL |
MS645 Related Classifications
HY-125232 Related Classifications
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