2. Apoptosis Epigenetics
  3. Apoptosis MDM-2/p53 Epigenetic Reader Domain
  4. Ischemin sodium

Ischemin sodium is a CBP bromodomain inhibitor that inhibits p53 interaction with CBP and transcriptional activity in cells. Ischemin sodium salt inhibits p53-induced p21 activation with an IC50 value of 5 µM. Ischemin sodium salt also prevents apoptosis in ischemic cardiomyocytes. Ischemin sodium salt can be used in the study of cardiovascular diseases (such as myocardial ischemia).

For research use only. We do not sell to patients.

Ischemin sodium Chemical Structure

Ischemin sodium Chemical Structure

Size Price Stock Quantity
5 mg USD 285 In-stock
10 mg USD 450 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Ischemin sodium Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Ischemin sodium is a CBP bromodomain inhibitor that inhibits p53 interaction with CBP and transcriptional activity in cells. Ischemin sodium salt inhibits p53-induced p21 activation with an IC50 value of 5 µM. Ischemin sodium salt also prevents apoptosis in ischemic cardiomyocytes. Ischemin sodium salt can be used in the study of cardiovascular diseases (such as myocardial ischemia)[1].

In Vitro

Ischemin sodium (50, 100 µM; 24 h) inhibits p53 activation on DNA damaging stress in U2OS cells[1].
Ischemin sodium (10 μM; 3 days) blocks apoptosis in cardiomyocytes by inhibiting caspase 3/7 activity[1].
Ischemin sodium salt inhibits p53 cellular signaling pathways in U2OS cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ischemin sodium Related Antibodies

Western Blot Analysis[1]

Cell Line: U2OS cells
Concentration: 50, 100 μM
Incubation Time: 24 h
Result: Inhibited the Doxorubicin-induced increased levels of p53 protein, its Ser15-phosphorylated (p53S15p) and Lys382-acetylated (p53K382ac).

Apoptosis Analysis[1]

Cell Line: U2OS cells
Concentration: 10 μM
Incubation Time: 3 days
Result: Functioned as a cellular protective agent againsted myocardial ischemic stress.
Molecular Weight

357.36

Formula

C15H16N3NaO4S
Appearance

Solid

Color

Light brown to brown

SMILES

CC1=CC(/N=N/C2=C(C=C(C(S(=O)(O[Na])=O)=C2)C)C)=C(C=C1O)N
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 36 mg/mL (100.74 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7983 mL 13.9915 mL 27.9830 mL
5 mM 0.5597 mL 2.7983 mL 5.5966 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.7983 mL 13.9915 mL 27.9830 mL 69.9575 mL
5 mM 0.5597 mL 2.7983 mL 5.5966 mL 13.9915 mL
10 mM 0.2798 mL 1.3991 mL 2.7983 mL 6.9957 mL
15 mM 0.1866 mL 0.9328 mL 1.8655 mL 4.6638 mL
20 mM 0.1399 mL 0.6996 mL 1.3991 mL 3.4979 mL
25 mM 0.1119 mL 0.5597 mL 1.1193 mL 2.7983 mL
30 mM 0.0933 mL 0.4664 mL 0.9328 mL 2.3319 mL
40 mM 0.0700 mL 0.3498 mL 0.6996 mL 1.7489 mL
50 mM 0.0560 mL 0.2798 mL 0.5597 mL 1.3991 mL
60 mM 0.0466 mL 0.2332 mL 0.4664 mL 1.1660 mL
80 mM 0.0350 mL 0.1749 mL 0.3498 mL 0.8745 mL
100 mM 0.0280 mL 0.1399 mL 0.2798 mL 0.6996 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Ischemin sodium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IscheminApoptosisMDM-2/p53Epigenetic Reader DomainU2OS293THEK293Tmyocardial ischemiap21apoptosiscardiomyocytesInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Ischemin sodium
Cat. No.:
HY-110315
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.