製品番号: HY-N0235

Bakuchiol

(別名: (S)-(+)-Bakuchiol)

2 Cited Publications

カスタマーレビュー

Based on 2 publication(s) in Google Scholar

Bakuchiol is a phytoestrogen that can be obtained from psoralen seeds. Bakuchiol has been proven to be a non-competitive inhibitor of multiple enzymes, including UDP-glucuronosyltransferase 2B7 (UGT2B7) and human carboxylesterase 2 (hCE2) , with IC₅₀s values of 40.9 μM and 7.28 μM, respectively. Bakuchiol exhibits significant research and application potential in areas such as anti-inflammatory, antibacterial, antitumor therapies, as well as drug metabolism regulation.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

純度: 98.08%
CAS 番号: 10309-37-2
分子式: C₁₈H₂₄O
分子量:256.38

保管条件:

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Bakuchiol 構造式

CAS 番号: 10309-37-2

容量

価格（税別）

在庫状況

数量

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性
- 製品説明
- IC₅₀ & Target
- Cellular Effect
- 体外実験
- 体内実験
- 臨床実験
化学情報
Publications (2)
溶剤 & 溶解度
- 体外
- 体内
純度とドキュメンテーション
参考文献
Help & FAQs
関連分類
Bioz

生物活性

製品説明

Bakuchiol is a phytoestrogen that can be obtained from psoralen seeds. Bakuchiol has been proven to be a non-competitive inhibitor of multiple enzymes, including UDP-glucuronosyltransferase 2B7 (UGT2B7) ^[2] and human carboxylesterase 2 (hCE2) ^[3], with IC₅₀s values of 40.9 μM and 7.28 μM, respectively. Bakuchiol exhibits significant research and application potential in areas such as anti-inflammatory^[5], antibacterial^[4], antitumor^[1] therapies, as well as drug metabolism regulation.

IC₅₀ & Target

IC₅₀:40.9 nM (UGT2B7) ; IC₅₀:7.28 nM (hCE2)

Carboxylesterase (CES) アイソフォーム固有の製品をすべて表示 :

p38 MAPK アイソフォーム固有の製品をすべて表示 :

Cellular Effect

Cell Line	Type	Value	Description	References
AGS	IC₅₀	15.3 μM Compound: 1	Cytotoxicity against human AGS cells by MTT assay Cytotoxicity against human AGS cells by MTT assay	[PMID: 18359631]
AGS	IC₅₀	6.1 μM Compound: 1	Inhibition of hypoxia-induced HIF1 activation in human AGS cells by reporter gene assay Inhibition of hypoxia-induced HIF1 activation in human AGS cells by reporter gene assay	[PMID: 18359631]
BJ	EC₅₀	>36.63 μM Compound: 1	Cytotoxicity against human BJ cells after 72 hrs by Cell-Titer Glo assay Cytotoxicity against human BJ cells after 72 hrs by Cell-Titer Glo assay	[PMID: 26922230]
HEK293	EC₅₀	>36.63 μM Compound: 1	Cytotoxicity against HEK293 cells after 72 hrs by Cell-Titer Glo assay Cytotoxicity against HEK293 cells after 72 hrs by Cell-Titer Glo assay	[PMID: 26922230]
HeLa	IC₅₀	11 μM Compound: 1	Cytotoxicity against human HeLa cells by MTT assay Cytotoxicity against human HeLa cells by MTT assay	[PMID: 18359631]
HeLa	IC₅₀	6.9 μM Compound: 1	Inhibition of TNF-alpha-induced NF-kappaB activation in human HeLa cells Inhibition of TNF-alpha-induced NF-kappaB activation in human HeLa cells	[PMID: 18359631]
HepG2	EC₅₀	>52.0833 μM Compound: 1	Cytotoxicity against human HepG2 cells after 72 hrs by Cell-Titer Glo assay Cytotoxicity against human HepG2 cells after 72 hrs by Cell-Titer Glo assay	[PMID: 26922230]
Jurkat	EC₅₀	>49.0196 μM Compound: 1	Cytotoxicity against human Jurkat cells after 72 hrs by Cell-Titer Glo assay Cytotoxicity against human Jurkat cells after 72 hrs by Cell-Titer Glo assay	[PMID: 26922230]
MDA-MB-231	IC₅₀	8.29 x 10^-3 mol/L Compound: 1	Cytotoxicity against human MDA-MB-231 cells Cytotoxicity against human MDA-MB-231 cells	[PMID: 22245048]
MDA-MB-231	IC₅₀	8.29*10^-3 M Compound: 1	Cytotoxicity against human MDA-MB-231 cells Cytotoxicity against human MDA-MB-231 cells	[PMID: 22245048]
NALM-6	EC₅₀	38 μM Compound: 1	Cytotoxicity against human NALM6 cells after 72 hrs by Cell-Titer Glo assay Cytotoxicity against human NALM6 cells after 72 hrs by Cell-Titer Glo assay	[PMID: 26922230]
Raji	EC₅₀	>26.0417 μM Compound: 1	Cytotoxicity against human Raji cells after 72 hrs by Cell-Titer Glo assay Cytotoxicity against human Raji cells after 72 hrs by Cell-Titer Glo assay	[PMID: 26922230]
RAW264.7	IC₅₀	97.23 μM Compound: BAK	Cytotoxicity against mouse RAW 264.7 cells assessed as decreased cell viability measured after 24 hrs by MTT assay Cytotoxicity against mouse RAW 264.7 cells assessed as decreased cell viability measured after 24 hrs by MTT assay	[PMID: 35000392]
SEM	EC₅₀	15.2866 μM Compound: 1	Cytotoxicity against human SEM cells after 72 hrs by Cell-Titer Glo assay Cytotoxicity against human SEM cells after 72 hrs by Cell-Titer Glo assay	[PMID: 26922230]
T47D	IC₅₀	2.89 x 10^-5 mol/L Compound: 1	Cytotoxicity against human T47D cells Cytotoxicity against human T47D cells	[PMID: 22245048]
T47D	IC₅₀	2.89*10^-5 M Compound: 1	Cytotoxicity against human T47D cells Cytotoxicity against human T47D cells	[PMID: 22245048]

体外実験

Bakuchiol (5-20 μM, 72 h) exhibits selected cytotoxicity in A549 cells, as determined by the MTT assay. Bakuchiol displays stronger effect than its analogue Resveratrol (HY-16561) in reducing the viability of A549 cells with IC₅₀s vales of 9.58 μM and 33.02 μM, respectively^[1].
Bakuchiol (5-20 μM, 24 h or 36h) blocks cell cycle progression at S phase and induction of Reactive Oxygen species (ROS) -related apoptosis (more importantly) in A549 cells in a concentration-dependent manner^[1].
Bakuchiol (0-80 μM, 120 min) is a non-competitive inhibitor of UDP-glucuronosyltransferase 2B7 (UGT2B7) with K_i value of 10.7 μM and IC₅₀ value of 40.9 μM^[2].
Bakuchiol (30 min) is a potent non-competitive inhibitor of human carboxylesterase 2 (hCE2) with low K_i value of 2.12 μM and IC₅₀ value of 7.28 μM^[3].
Bakuchiol (0.9775-3.91 μg/mL, 24 h) demonstrates a significant dose-dependent increase in membrane permeability of the fungal conidia of T. mentagrophytes^[4].
Bakuchiol (3.91 μg/mL, 3 h) elicites a 187% elevation in Reactive Oxygen species (ROS) level in fungal cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

A549 cells, EA.hy926 cells, HUVEC cells and MEF
Concentration:

5, 10, and 20 μM
Incubation Time:

24 hours
Result:

Induced caspase 9/3 activaton, p53 and Bax up-regulation, as well as Bcl-2 down-regulation.

Apoptosis Analysis^[1]

Cell Line:

A549 cells, EA.hy926 cells, HUVEC cells and MEF
Concentration:

5, 10, and 20 μM
Incubation Time:

36 hours
Result:

Resulted in typical apoptotic cells (p < 0.05), as determined by Annexin V/propidium iodide staining and flow cytometry.

Cell Cycle Analysis^[1]

Cell Line:

A549 cells
Concentration:

5, 10, and 20 μM
Incubation Time:

24 hours
Result:

Increased S phase cell population accompanied with a concomitant reduction of cells in the G1 phase. Resulted in a less potent effect in S phase arrest than bakuchiol.

Cell Cytotoxicity Assay^[1]

Cell Line:

A549 cells, EA.hy926 cells, HUVEC cells and MEF
Concentration:

5, 10, and 20 μM
Incubation Time:

72 hours
Result:

Inhibited the growth of A549 cells, while the growth rate of other cells were not changed. Exhibited concentration-dependent cytotoxicity in A549 cells, while bakuchiol showed a more potent effect than that of resveratrol with IC₅₀s vales of 9.58 μmol/L and 33.02 μmol/ L, respectively.

体内実験

Bakuchiol (10 and 20 mg/kg, p.o.) can attenuate allergic symptoms, decrease the inflammatory response, improve the T-cell balance, reduce oxidative stress and regulated Igs levels in ovalbumin (OVA) -induced allergic rhinitis (AR) mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

OVA -induced AR model (BALB/c mice, 6-week-old) ^[5]
Dosage:

10 and 20 mg/kg
Administration:

Oral route (p.o.)
Result:

Reduced the nasal symptoms and decreased the levels of IL-4, IL-5, IL-13, Igs (IgE and IgG1), histamine, IL-10, IL-33, and TNF-α. Reduced PGDA and LTC-4 levels in the nasal lavage fluid (NLF). Decreased the ROS and MDA levels, whereas boosting SOD activity. Decreased the eosinophil count in the nasal tissues and influenced the Th1 and Th2 cell proportions.

臨床実験

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

化学情報

CAS 番号 10309-37-2
性状 Liquid (Density: 0.963 g/cm³)
分子量 256.38
分子式 C₁₈H₂₄O
Color Colorless to yellow
SMILES

OC1=CC=C(/C=C/[C@](C)(C=C)CC/C=C(C)\C)C=C1
別名

(S)-(+)-Bakuchiol
Structure Classification
- Phenols
- Monophenols
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (2)

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Journal Impact Factor

J Ethnopharmacol

Discovery of bakuchiol as an AIM2 inflammasome activator and cause of hepatotoxicity. [Abstract]

2022 Nov 15:298:115593. PMID: 35973629
Chem Biol Interact

Bavachinin, a main compound of Psoraleae Fructus, facilitates GSDMD-mediated pyroptosis and causes hepatotoxicity in mice. [Abstract]

2024 Sep 1:400:111133. PMID: 38969277

溶剤 & 溶解度

体外:

DMSO : 62.5 mg/mL (243.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.9005 mL

19.5023 mL

39.0046 mL

5 mM

0.7801 mL

3.9005 mL

7.8009 mL

10 mM

0.3900 mL

1.9502 mL

3.9005 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.17 mg/mL (8.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.17 mg/mL (8.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

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COA (2659 KB)
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HNMR (2659 KB)
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RP-HPLC (2659 KB)
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MS (2659 KB)
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OR (2659 KB)
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SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Chen Z, et al. Anti-tumor effects of bakuchiol, an analogue of resveratrol, on human lung adenocarcinoma A549 cell line. Eur J Pharmacol. 2010 Sep 25;643(2-3):170-9. [Content Brief]

[2]. Xu Y, et al. In vitro evidence for bakuchiol's influence towards drug metabolism through inhibition of UDP-glucuronosyltransferase (UGT) 2B7. Afr Health Sci. 2014 Sep;14(3):564-9. [Content Brief]

[3]. Li YG, et al. Fructus Psoraleae contains natural compounds with potent inhibitory effects towards human carboxylesterase 2. Fitoterapia. 2015 Jan 13;101C:99-106. [Content Brief]

[4]. Lau KM, et al. Anti-dermatophytic activity of bakuchiol: in vitro mechanistic studies and in vivo tinea pedis-inhibiting activity in a guinea pig model. Phytomedicine. 2014 Jun 15;21(7):942-5. [Content Brief]

[5]. Yao HB, et al. Anti-Allergic and Anti-inflammatory Effects of Bakuchiol on Ovalbumin-Induced Allergic Rhinitis in Mice. Appl Biochem Biotechnol. 2024;196(6):3456-3470. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.9005 mL	19.5023 mL	39.0046 mL	97.5115 mL
	5 mM	0.7801 mL	3.9005 mL	7.8009 mL	19.5023 mL
	10 mM	0.3900 mL	1.9502 mL	3.9005 mL	9.7512 mL
	15 mM	0.2600 mL	1.3002 mL	2.6003 mL	6.5008 mL
	20 mM	0.1950 mL	0.9751 mL	1.9502 mL	4.8756 mL
	25 mM	0.1560 mL	0.7801 mL	1.5602 mL	3.9005 mL
	30 mM	0.1300 mL	0.6501 mL	1.3002 mL	3.2504 mL
	40 mM	0.0975 mL	0.4876 mL	0.9751 mL	2.4378 mL
	50 mM	0.0780 mL	0.3900 mL	0.7801 mL	1.9502 mL
	60 mM	0.0650 mL	0.3250 mL	0.6501 mL	1.6252 mL
	80 mM	0.0488 mL	0.2438 mL	0.4876 mL	1.2189 mL
	100 mM	0.0390 mL	0.1950 mL	0.3900 mL	0.9751 mL

Bioz

Keywords

Bakuchiol

2 Cited Publications

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Bakuchiol

Psoralen

Bavachin

Angelicin

Gemcitabine

Nicotinamide

Bakuchiol (Standard)

Cholesterol (from animal)

Isorhamnetin

Parthenolide

Cryptotanshinone

Asiatic acid

Cholesterol (Standard)

MedChemExpress（MCE）の使用を引用している文献 Bakuchiol

Carboxylesterase (CES) アイソフォーム固有の製品をすべて表示

p38 MAPK アイソフォーム固有の製品をすべて表示

生物活性

化学情報

Publications (2)

溶剤 & 溶解度

純度とドキュメンテーション

参考文献

Help & FAQs

Complete Stock Solution Preparation Table

Bioz

Bakuchiol

Psoralen

Bavachin

Angelicin

Gemcitabine

Nicotinamide

Bakuchiol (Standard)

Cholesterol (from animal)

Isorhamnetin

Parthenolide

Cryptotanshinone

Asiatic acid

Cholesterol (Standard)

MedChemExpress（MCE）の使用を引用している文献 Bakuchiol

Carboxylesterase (CES) アイソフォーム固有の製品をすべて表示

p38 MAPK アイソフォーム固有の製品をすべて表示

生物活性

化学情報

Publications (2)

溶剤 & 溶解度

純度とドキュメンテーション

参考文献

Help & FAQs

Complete Stock Solution Preparation Table

Bakuchiol 関連分類

HY-N0235 関連分類

Bioz