製品番号: HY-50737

DUB-IN-3

7 Cited Publications

カスタマーレビュー

Based on 7 publication(s) in Google Scholar

DUB-IN-3 (compound 22c) is a potent and selective inhibitor of the deubiquitinating enzyme USP8 with an IC₅₀ value of 0.56 μM. DUB-IN-3 is promising for research of cancer, neurodegenerative diseases, inflammation, viral infection and cardiovascular disease.

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純度: 99.00%
CAS 番号: 924296-17-3
分子式: C₁₆H₉N₅O
分子量:287.28

保管条件:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

DUB-IN-3 構造式

CAS 番号: 924296-17-3

容量

価格（税別）

在庫状況

数量

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

IC₅₀ & Target

USP7

>100 μM (IC₅₀)

Deubiquitinase アイソフォーム固有の製品をすべて表示 :

体外実験

DUB-IN-3 (10 nM-100 μM) affects the viability of HCT116 colon and PC-3 prostate cancer cell lines with IC₅₀ values of 0.5-1.5 μM range^[1].

Inhibitory effect of DUB-IN-3 on USP7 and USP8 deubiquitinating activity (IC₅₀: μM)^[1]

Compd	USP7	USP8
DUB-IN-3	>100	0.56

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

化学情報

CAS 番号 924296-17-3
性状 Solid
分子量 287.28
分子式 C₁₆H₉N₅O
Color Light yellow to yellow
SMILES

C=CCO/N=C1C2=C(N=C(C#N)C(C#N)=N2)C3=CC=CC=C/13
輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (7)

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Cell Res

Targeted destruction of VISTA boosts anti-tumor immunotherapy. [Abstract]

2025 Dec;35(12):987-1002. PMID: 41225154
Cell Death Dis

Usp8 promotes tumor cell migration through activating the JNK pathway. [Abstract]

2022 Mar 31;13(3):286. PMID: 35361778
J Med Chem

Discovery of Potent OTUB1/USP8 Dual Inhibitors Targeting Proteostasis in Non-Small-Cell Lung Cancer. [Abstract]

2022 Oct 27;65(20):13645-13659. PMID: 36221183
Cancer Cell Int

USP8 promotes the tumorigenesis of intrahepatic cholangiocarcinoma via stabilizing OGT. [Abstract]

2024 Jul 7;24(1):238. PMID: 38973004
J Invest Dermatol

The Identification of Potential Therapeutic Targets for Cutaneous Squamous Cell Carcinoma. [Abstract]

2020 Jun;140(6):1154-1165.e5. PMID: 31705877

DUB-IN-3 purchased from MedChemExpress. Usage Cited in: J Invest Dermatol. 2020 Jun;140(6):1154-1165.e5. [Abstract]

Western blot analysis of SCCRDEB4 cells treated with the USP8 inhibitor DUBs-IN-3 for 24 hours.
Mediators Inflamm

GPR34 Stabilized by Deubiquitinase USP8 Suppresses Ferroptosis of ATC. [Abstract]

2025 Aug 18:2025:5576056. PMID: 40862294
Arch Pharm (Weinheim)

Synthesis and structure-activity relationships of USP48 deubiquitinylase inhibitors. [Abstract]

2023 Jul;356(7):e2200661. PMID: 37196427

溶剤 & 溶解度

体外:

DMSO : 25 mg/mL (87.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.4809 mL

17.4046 mL

34.8092 mL

5 mM

0.6962 mL

3.4809 mL

6.9618 mL

10 mM

0.3481 mL

1.7405 mL

3.4809 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (277 KB)
SDS (544 KB)
- English - EN (544 KB)
- Français - FR (544 KB)
- Deutsch - DE (544 KB)
- Norwegian - NO (544 KB)
- Español - ES (544 KB)
- Swedish - SV (544 KB)
- Italian - IT (544 KB)
- Korean - KR (544 KB)
- Portuguese - PT (544 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Colombo M, et al. Synthesis and biological evaluation of 9-oxo-9H-indeno[1,2-b]pyrazine-2,3-dicarbonitrile analogues as potential inhibitors of deubiquitinating enzymes. ChemMedChem. 2010 Apr 6;5(4):552-8. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.4809 mL	17.4046 mL	34.8092 mL	87.0231 mL
	5 mM	0.6962 mL	3.4809 mL	6.9618 mL	17.4046 mL
	10 mM	0.3481 mL	1.7405 mL	3.4809 mL	8.7023 mL
	15 mM	0.2321 mL	1.1603 mL	2.3206 mL	5.8015 mL
	20 mM	0.1740 mL	0.8702 mL	1.7405 mL	4.3512 mL
	25 mM	0.1392 mL	0.6962 mL	1.3924 mL	3.4809 mL
	30 mM	0.1160 mL	0.5802 mL	1.1603 mL	2.9008 mL
	40 mM	0.0870 mL	0.4351 mL	0.8702 mL	2.1756 mL
	50 mM	0.0696 mL	0.3481 mL	0.6962 mL	1.7405 mL
	60 mM	0.0580 mL	0.2901 mL	0.5802 mL	1.4504 mL
	80 mM	0.0435 mL	0.2176 mL	0.4351 mL	1.0878 mL

Keywords

DUB-IN-3

7 Cited Publications

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DUB-IN-3

Anti-Infection Compound Library

Cell Cycle/DNA Damage Compound Library

Immunology/Inflammation Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Anti-Cardiovascular Disease Compound Library

Ubiquitination Compound Library

Neurodegenerative Disease-related Compound Library

Metabolic Enzyme Compound Library

Protease Inhibitor Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

MedChemExpress（MCE）の使用を引用している文献 DUB-IN-3

Deubiquitinase アイソフォーム固有の製品をすべて表示

生物活性

化学情報

Publications (7)

溶剤 & 溶解度

純度とドキュメンテーション

参考文献

Help & FAQs

Complete Stock Solution Preparation Table

DUB-IN-3

Anti-Infection Compound Library

Cell Cycle/DNA Damage Compound Library

Immunology/Inflammation Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Anti-Cardiovascular Disease Compound Library

Ubiquitination Compound Library

Neurodegenerative Disease-related Compound Library

Metabolic Enzyme Compound Library

Protease Inhibitor Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

MedChemExpress（MCE）の使用を引用している文献 DUB-IN-3

Deubiquitinase アイソフォーム固有の製品をすべて表示

生物活性

化学情報

Publications (7)

溶剤 & 溶解度

純度とドキュメンテーション

参考文献

Help & FAQs

Complete Stock Solution Preparation Table

DUB-IN-3 関連分類

HY-50737 関連分類