Dentonin TFA
Dentonin TFA (AC-100 TFA) is a synthetic fragment derived from MEPE. Dentonin TFA enhances osteogenesis by promoting osteoprogenitor adhesion and facilitates immature adherent cells survival. Dentonin TFA has no significant effect to mature osteoblasts. Dentonin TFA can be used for the research of phosphate homeostasis and bone metabolism.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 分子式: C109H161F3N30O44
- 分子量:2652.61
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
MEPE is a a member of the SIBLING (Small Integrin-Binding Ligand, N-linked Glycoprotein) family of secreted glycophosphoproteins.? MEPE ?regulates bone mass and influence osteoblast activity.[1].Dentonin TFA (3-30 μg/ml; 2-24 hours) significantly increases the numbers of cell and shows enhanced promotion of cell adhesion. However, it has no significant differences in terms of numbers of cells adhered[2].Dentonin TFA (3-30 μg/ml; 2-24 hours) enhances osteoblast spreading, it exhibits significantly increased cell areas compared to all other treatment[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
化学情報
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分子量 2652.61
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分子式 C109H161F3N30O44
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別名
AC-100 TFA
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シーケンスの短縮
TDLQERGDNDISPFSGDGQPFKD
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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Mesenchymal stromal/stem cell osteogenic differentiation
Mesenchymal stromal/stem cells can be induced toward an osteoblast-like lineage in vitro by culture in osteogenic medium containing dexamethasone, ascorbic acid or ascorbate-2-phosphate, and β-glycerophosphate; the differentiation process is commonly evaluated by alkaline phosphatase activity, osteogenic marker expression, collagenous matrix formation, and calcium-rich matrix mineralization. The main readouts are alkaline phosphatase activity as an early osteogenic marker and Alizarin Red S staining as a calcium-deposit readout for mineralized extracellular matrix; Alizarin Red S can be inspected microscopically or extracted and measured colorimetrically at 405 nm.
純度とドキュメンテーション
参考文献
[1]. Andrew P Sprowson, et al. ASARM-truncated MEPE and AC-100 enhance osteogenesis by promoting osteoprogenitor adhesion. J Orthop Res. 2008 Sep;26(9):1256-62. [Content Brief]
[2]. N Six, et al. Dentonin, a MEPE fragment, initiates pulp-healing response to injury.J Dent Res. 2007 Aug;86(8):780-5. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Dentonin TFA 関連分類
HY-P2633A 関連分類
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