Cat. No.: HY-N0208

Kaempferol-3-O-glucorhamnoside

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Kaempferol-3-O-glucorhamnoside is a flavonoid compound. Kaempferol-3-O-glucorhamnoside can be extracted from T. chinense Turcz. Kaempferol-3-O-glucorhamnoside inhibits the phosphorylation of NF-κB and MAPK kinases. Kaempferol-3-O-glucorhamnoside suppresses the expression of cytokines (TNF-α, IL-6, IL-1β and PGE₂) and reduces ROS levels. Kaempferol-3-O-glucorhamnoside ameliorates pulmonary edema and alleviates oxidative stress in the lungs and cells of mice exposed to Klebsiella pneumoniae. Kaempferol-3-O-glucorhamnoside possesses anti-inflammatory properties. Kaempferol-3-O-glucorhamnoside can be used in studies related to Klebsiella pneumoniae infection (pneumonia).

For research use only. We do not sell to patients.

Purity: 99.64%
CAS No.: 40437-72-7
Formula: C₂₇H₃₀O₁₅
Molecular Weight:594.52

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Kaempferol-3-O-glucorhamnoside Chemical Structure

CAS No.: 40437-72-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All p38 MAPK Isoforms :

All NF-κB Isoforms :

All TNF Receptor Isoforms :

All Interleukin Related Isoforms :

In Vitro

Kaempferol-3-O-glucorhamnoside (1-4 μg/mL; 10 h) does not inhibit the growth of *Klebsiella pneumoniae* (CICC-10870), which is evidenced by the results that its MIC >256 μg/mL and there is no significant difference in growth curves compared with the control group^[1].
Kaempferol-3-O-glucorhamnoside (1-4 μg/mL; 1 h) protects mouse RAW 264.7 macrophages from Klebsiella pneumoniae-induced cell death in a concentration-dependent manner, and exhibits no intrinsic cytotoxicity at concentrations of 1, 2, and 4 μg/mL^[1].
Kaempferol-3-O-glucorhamnoside (1-4 μg/mL; 1 h) dose-dependently reduces Klebsiella pneumoniae-induced reactive oxygen species production in mouse RAW 264.7 macrophages at concentrations of 1, 2, and 4 μg/mL^[1].
Kaempferol-3-O-glucorhamnoside (1-4 μg/mL; administered during *Klebsiella pneumoniae* incubation) dose-dependently inhibits the phosphorylation of p38, ERK, JNK, IκB and NF-κB p65 in mouse RAW 264.7 macrophages exposed to *Klebsiella pneumoniae*, thereby suppressing the activation of MAPK and NF-κB pathways^[1].
Kaempferol-3-O-glucorhamnoside downregulates the expression of pro-inflammatory mediators in LPS-induced RAW264.7 macrophages by inhibiting the MAPK and NF-κB signaling pathways^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

murine RAW 264.7 macrophages
Concentration:

1-4 μg/mL (viability assay; infection protection assay)
Incubation Time:

1 h (viability assay); 1 h pre-incubation before 24 h Klebsiella pneumoniae infection (protection assay)
Result:

Had no significant effect on RAW 264.7 cell viability at tested concentrations.
Protected cells from Klebsiella pneumoniae-induced viability reduction in a concentration-dependent manner, with higher concentrations correlating with greater cell survival.

Western Blot Analysis^[1]

Cell Line:

murine RAW 264.7 macrophages
Concentration:

1-4 μg/mL
Incubation Time:

during Klebsiella pneumoniae incubation
Result:

Dose-dependently suppressed the phosphorylation of p38, ERK, JNK, IκB, and NF-κB p65 induced by Klebsiella pneumoniae infection, with significant reductions observed at 2 and 4 μg/mL for most targets.

In Vivo

Kaempferol-3-O-glucorhamnoside (50-200 μg/kg; i.p.; single injection) dose-dependently inhibits Klebsiella pneumoniae-induced inflammatory responses and oxidative stress in mice via suppression of MAPK and NF-κB pathway phosphorylation, significantly reducing lung edema, proinflammatory cytokine levels, and inflammatory cell infiltration^[1].
Kaempferol-3-O-glucorhamnoside (200 μg/kg; i.p.; single injection) does not induce inflammatory or oxidative stress changes in healthy BALB/c mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c (male, 6-weeks old, 18-22 g, intranasal challenge with 1×10⁷ cfu Klebsiella pneumoniae)^[1]
Dosage:

50 μg/kg; 100 μg/kg; 200 μg/kg
Administration:

i.p.; single injection
Result:

Reduced lung edema dose-dependently, with the 200 μg/kg dose yielding effects similar to 50% of the positive control.
Decreased inflammatory cell accumulation and alveolar hemorrhaging in lung histopathology dose-dependently.
Suppressed bronchoalveolar lavage fluid levels of TNF-α, IL-6, IL-1β, and PGE2 dose-dependently, with significant reductions (P < 0.05 or P < 0.01) at all tested doses.
Reduced total infiltrated cells, neutrophils, and macrophages in bronchoalveolar lavage fluid dose-dependently, with significant decreases (P < 0.05 or P < 0.01) at all tested doses.
Decreased lung tissue levels of oxidative stress markers MPO and MDA dose-dependently, with significant changes (P < 0.05 or P < 0.01) at all tested doses.
Increased lung tissue levels of antioxidant markers SOD and GSH dose-dependently, with significant changes (P < 0.05 or P < 0.01) at all tested doses.
Suppressed phosphorylation of MAPK pathway proteins (p38, ERK, JNK) and NF-κB pathway proteins (IκB, NF-κB p65) in lung tissue dose-dependently, with significant reductions (P < 0.05 or P < 0.01) at all tested doses.

Animal Model:

BALB/c (male, 6-weeks old, 18-22 g)^[1]
Dosage:

200 μg/kg
Administration:

i.p.; single injection
Result:

Showed no significant changes in bronchoalveolar lavage fluid cytokine levels (TNF-α, IL-6, IL-1β, PGE2) compared to saline control mice.
Showed no significant changes in lung oxidative stress markers (MPO, MDA, SOD, GSH) compared to saline control mice.
Showed no significant changes in lung edema compared to saline control mice.

Chemical Information

CAS No. 40437-72-7
Appearance Solid
Molecular Weight 594.52
Formula C₂₇H₃₀O₁₅
Color Light yellow to yellow
SMILES

C[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O[C@@H]2[C@H](O)[C@@H](O)[C@H](CO)O[C@H]2OC3=C(C4=CC=C(O)C=C4)OC5=CC(O)=CC(O)=C5C3=O)O1
Structure Classification
- Flavonoids
- Flavonols
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (168.20 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6820 mL

8.4101 mL

16.8203 mL

5 mM

0.3364 mL

1.6820 mL

3.3641 mL

10 mM

0.1682 mL

0.8410 mL

1.6820 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (286 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Sun Z, et al. Kaempferol-3-O-glucorhamnoside inhibits inflammatory responses via MAPK and NF-κB pathways in vitro and in vivo. Toxicology and applied pharmacology. 2019 Feb 01;364:22-28. [Content Brief]

[2]. Chen M, et al. Kaempferol and atherosclerosis: From mechanism to medicine. Critical reviews in food science and nutrition. 2024;64(8):2157-2175. [Content Brief]

[3]. Wang L, et al. Integrative study of Anchusa italica Retz flower's microstructure and chemical composition. Scientific reports. 2025 Feb 21;15(1):6366. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	1.6820 mL	8.4101 mL	16.8203 mL	42.0507 mL
	5 mM	0.3364 mL	1.6820 mL	3.3641 mL	8.4101 mL
	10 mM	0.1682 mL	0.8410 mL	1.6820 mL	4.2051 mL
	15 mM	0.1121 mL	0.5607 mL	1.1214 mL	2.8034 mL
	20 mM	0.0841 mL	0.4205 mL	0.8410 mL	2.1025 mL
	25 mM	0.0673 mL	0.3364 mL	0.6728 mL	1.6820 mL
	30 mM	0.0561 mL	0.2803 mL	0.5607 mL	1.4017 mL
	40 mM	0.0421 mL	0.2103 mL	0.4205 mL	1.0513 mL
	50 mM	0.0336 mL	0.1682 mL	0.3364 mL	0.8410 mL
	60 mM	0.0280 mL	0.1402 mL	0.2803 mL	0.7008 mL
	80 mM	0.0210 mL	0.1051 mL	0.2103 mL	0.5256 mL
	100 mM	0.0168 mL	0.0841 mL	0.1682 mL	0.4205 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Kaempferol-3-O-glucorhamnoside

Bioactive Compound Library Max

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Anti-Infection Compound Library

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All p38 MAPK Isoforms

All NF-κB Isoforms

All TNF Receptor Isoforms

All Interleukin Related Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Kaempferol-3-O-glucorhamnoside Related Classifications

HY-N0208 Related Classifications