Cat. No.: HY-174866

BT-O2C

Name: BT-O2C
Brand: MedChemExpress
SKU: HY-174866
Price: 1425.00 USD
Availability: InStock
Rating: 5 (1 reviews)

고객리뷰

Based on 1 Customer Validation

BT-O2C is a p300 PROTAC degrader. BT-O2C induces p300 degradation via proteasome- and ubiquitin-like modification-dependent pathways, downregulates the expression of CIC::DUX4 sarcoma target genes, and exerts cytotoxicity against such sarcoma cells. BT-O2C can be used in CIC::DUX4 sarcoma-related studies.
(Pink: p300 ligand (HY-174868); Blue: Cereblon ligand (HY-W023573); Black: linker).

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity: 99.31%
CAS No.: 3055107-34-8
화학식: C₄₈H₄₈F₅N₉O₈
분자량:973.94

보관:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

BT-O2C Chemical Structure

CAS No.: 3055107-34-8

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
용액&용해도
- In Vitro
- In Vivo
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

BT-O2C is a p300 PROTAC degrader. BT-O2C induces p300 degradation via proteasome- and ubiquitin-like modification-dependent pathways, downregulates the expression of CIC::DUX4 sarcoma target genes, and exerts cytotoxicity against such sarcoma cells. BT-O2C can be used in CIC::DUX4 sarcoma-related studies^[1]. (Pink: p300 ligand (HY-174868); Blue: Cereblon ligand (HY-W023573); Black: linker).

IC₅₀ & Target

^[1]

p300

All PROTACs Isoforms :

All Histone Acetyltransferase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
NCC	IC₅₀	152 nM	Cytotoxic activity against human NCC-CDS-X1 CIC::DUX4 sarcoma cells assessed as reduction in cell viability incubated for 72 hrs by CellTiter-Glo Luminescent Cell Viability Assay measuring ATP levels. Cytotoxic activity against human NCC-CDS-X1 CIC::DUX4 sarcoma cells assessed as reduction in cell viability incubated for 72 hrs by CellTiter-Glo Luminescent Cell Viability Assay measuring ATP levels.	40093518

In Vitro

BT-O2C (72 h) exhibits cytotoxicity against the CIC::DUX4 sarcoma cell lines NCC-CDS-X1 and KITRA, with IC₅₀ values of 152 nM and 221 nM respectively after 72 hours of treatment^[1].
BT-O2C (0.1-10 μM; 24-48 h) potently and selectively induces the degradation of p300 in HAP1 cells, and exhibits higher selectivity for p300 degradation over CBP^[1].
BT-O2C (4 μM; 6 h) reduces the expression of CIC::DUX4 target genes ETV1, ETV4 and ETV5 in NCC-CDS-X1 cells, with no statistically significant difference compared with its negative control BT-O2C-N^[1].
BT-O2C (4 μM; 24 h) slightly reduces the acetylation level of H3K27 in NCC-CDS-X1 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

CIC::DUX4 sarcoma (CDS) cell lines NCC-CDS-X1 and KITRA
Concentration:

0.25, 1.0, 4.0 μM
Incubation Time:

72 h
Result:

Exhibited cytotoxic activity in both CDS cell lines.
Achieved an IC₅₀ of 152 nM in NCC-CDS-X1 cells after 72-hour incubation.
Achieved an IC₅₀ of 221 nM in KITRA cells after 72-hour incubation.

Real Time qPCR^[1]

Cell Line:

NCC-CDS-X1 cells
Concentration:

4 μM
Incubation Time:

6 h
Result:

Reduced expression of CDS target genes ETV1, ETV4, and ETV5.
Showed no statistically significant difference in gene expression reduction compared to its negative control BT-O2C-N.

Western Blot Analysis^[1]

Cell Line:

NCC-CDS-X1 cells
Concentration:

4 μM
Incubation Time:

24 h
Result:

Caused a slight decrease in H3K27 acetylation.
Showed no clear effect on H3K18 acetylation.

Chemical Information

CAS No. 3055107-34-8
Appearance Solid
분자량 973.94
화학식 C₄₈H₄₈F₅N₉O₈
Color Light yellow to yellow
SMILES

O=C(N(CC1=CC=C(F)C=C1)[C@@H](C)C(F)(F)F)CN(C(N[C@]23C[C@@H](F)C4=C3C=CC(C5=CN(CC(NCCCCCCCNC6=CC7=C(C(N(C(CC8)C(NC8=O)=O)C7=O)=O)C=C6)=O)N=C5)=C4)=O)C2=O
선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : 100 mg/mL (102.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.0268 mL

5.1338 mL

10.2676 mL

5 mM

0.2054 mL

1.0268 mL

2.0535 mL

10 mM

0.1027 mL

0.5134 mL

1.0268 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (2.57 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Marsh GP, et al. Development of p300-targeting degraders with enhanced selectivity and onset of degradation. RSC medicinal chemistry. 2025 May 22;16(5):2049-2060. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.0268 mL	5.1338 mL	10.2676 mL	25.6689 mL
	5 mM	0.2054 mL	1.0268 mL	2.0535 mL	5.1338 mL
	10 mM	0.1027 mL	0.5134 mL	1.0268 mL	2.5669 mL
	15 mM	0.0685 mL	0.3423 mL	0.6845 mL	1.7113 mL
	20 mM	0.0513 mL	0.2567 mL	0.5134 mL	1.2834 mL
	25 mM	0.0411 mL	0.2054 mL	0.4107 mL	1.0268 mL
	30 mM	0.0342 mL	0.1711 mL	0.3423 mL	0.8556 mL
	40 mM	0.0257 mL	0.1283 mL	0.2567 mL	0.6417 mL
	50 mM	0.0205 mL	0.1027 mL	0.2054 mL	0.5134 mL
	60 mM	0.0171 mL	0.0856 mL	0.1711 mL	0.4278 mL
	80 mM	0.0128 mL	0.0642 mL	0.1283 mL	0.3209 mL
	100 mM	0.0103 mL	0.0513 mL	0.1027 mL	0.2567 mL

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BT-O2C

All PROTACs Isoforms

All Histone Acetyltransferase Isoforms