ddATP
Based on 1 publication(s) in Google Scholar
ddATP (2',3'-Dideoxyadenosine 5'-triphosphate), an active metabolite of 2',3'-dideoxyinosine, is a chain-elongating inhibitor of DNA polymerase. ddATP can be used for Sanger method for DNA sequencing and research of virus infection.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- CAS No.: 24027-80-3
- 화학식: C10H16N5O11P3
- 분자량:475.18
-
보관:
Solution, -20°C, 2 years
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocol
- 순도&문서
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) ddATP
MoreAll DNA/RNA Synthesis Isoforms
More
Biological Activity
제품 설명
IC50 & Target
|
DNA Polymerase |
In Vitro
ddATP (0.05-50 μM, 30 min) inhibits adenovirus DNA synthesis in isolated nuclei from adenovirus-infected KB cells[1].
ddATP (100 μM, 5 h) reduces the number of γH2A.X foci in the DSB (DNA double-strand breaks) oocytes[3].
ddATP inhibits HIV replication by inhibiting HIV reverse transcriptase[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
-
Cell Line:DSB oocytes
-
Concentration:100 μM
-
Incubation Time:5 h
-
Result:Suppressed the mtDNA replication without fully suppressed the nuclear DNA replication.
Chemical Information
-
CAS No. 24027-80-3
-
Appearance Liquid
-
분자량 475.18
-
화학식 C10H16N5O11P3
-
Color Colorless to light yellow
-
SMILES
NC1=NC=NC2=C1N=CN2[C@H]3CC[C@H](O3)COP(O)(OP(OP(O)(O)=O)(O)=O)=O
-
Synonyms
2',3'-Dideoxyadenosine 5'-triphosphate
-
선적
Room temperature in continental US; may vary elsewhere.
-
보관
Solution, -20°C, 2 years
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
RNA
Improved precision, sensitivity, and adaptability of Ordered Two-Template Relay cDNA library preparation for RNA sequencing. [Abstract]2025 Jan 22;31(2):224-244. PMID: 39626888
Protocol
-
Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
순도&문서
References
[1]. van der Vliet PC, et al. Role of DNA polymerase gamma in adenovirus DNA replication. Mechanism of inhibition by 2',3'-dideoxynucleoside 5'-triphosphates. Biochemistry. 1981 Apr 28;20(9):2628-32. [Content Brief]
[2]. Xu Lan, et al. Simultaneous determination of 2 ,3 -dideoxyinosine and the active metabolite, 2 ,3 -dideoxyadenosine-5 -triphosphate in human peripheral-blood mononuclear cell by HPLC–MS/MS and the application to cell pharmacokinetics. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Oct 1;1002:337-42. [Content Brief]
[3]. Jun-Yu Ma, et al. Double-strand breaks induce short-scale DNA replication and damage amplification in the fully grown mouse oocytes. Genetics. 2021 Jun 24;218(2):iyab054. [Content Brief]
[4]. C M Perry, et al. Didanosine. An update on its antiviral activity, pharmacokinetic properties and therapeutic efficacy in the management of HIV disease. Drugs. 1996 Dec;52(6):928-62. [Content Brief]
[5]. Hao Z, et al. Factors determining the activity of 2',3'-dideoxynucleosides in suppressing human immunodeficiency virus in vitro. Mol Pharmacol. 1988 Oct;34(4):431-5. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
ddATP Related Classifications
HY-128036 Related Classifications
Powered by Bioz