Hypaphorine
Based on 2 publication(s) in Google Scholar
Hypaphorine is an indole found in Caragana korshinskii. Hypaphorine has neurological and glucose-lowering effects. Hypaphorine prevents Lipopolysaccharides (LPS) (HY-D1056)-mediated acute lung injury (ALI) and proinflammatory response via inactivating the p38/JNK signaling pathway by upregulating DUSP1.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity: 99.93%
- CAS No.: 487-58-1
- 화학식: C14H18N2O2
- 분자량:246.31
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보관:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Hypaphorine
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Biological Activity
Hypaphorine (6.25-100 μM, 24 h) inhibits the Lipopolysaccharides (LPS) (HY-D1056)-mediated apoptosis of alveolar epithelial cells[2].
Hypaphorine (12.5-50 μM, 48 h) attenuates the expression of inflammatory factors (IL-1β, IL-6, TNF-α, and IL-18) and inactivates the p38/JNK signaling pathway through up-regulating DUSP1 in a dose-dependent manner[2].
Hypaphorine reverses inhibition of E. globulus seedling root growth induced by 3-Indoleacetic acid (Indole-3-acetic acid) (HY-18569)[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:BEAS-2B lung epithelial cells
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Concentration:6.25, 12.5, 25, 50, 100 μM
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Incubation Time:24 h
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Result:Cell viability was not changed when the dose was less than 50 μM, cell viability decreased significantly at 100 μM.
Cell viability was elevated dose-dependently (12.5, 25, 50 μM) in an ALI model.
The expression of pro-apoptotic proteins Bax and Caspase3 was dampened, while the anti-apoptotic protein Bcl2 was increased significantly in a dose-dependent manner.
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Cell Line:BEAS-2B lung epithelial cells
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Concentration:12.5, 25, 50 μM
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Incubation Time:48 h
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Result:Promoted the level of DUSP1 mRNA in dose-dependent manner.
DUSP1 was significantly upregulated after LPS treatment.
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Cell Line:BEAS-2B lung epithelial cells
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Concentration:12.5, 25, 50 μM
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Incubation Time:48 h
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Result:Distinctly strengthened the DUSP1 expression and inactivated p38/JNK pathway.
Enhanced DUSP1 protein level and inactivated the p38/JNK signaling pathway after LPS treatment.
Hypaphorine (50 mg/kg; i.p.; once) has antihyperglycemic activity[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male albino rats of Wistar/Sprague-Dawley strain (8-10 weeks, 160 g) made diabetic by intraperitoneal injection of Streptozotocin (HY-13753)[4]
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Dosage:50 mg/kg
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Administration:Intraperitoneal injection (i.p.); once
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Result:Showed significant anti-hyperglycemic activity (27.8% at 5 h and 20.4% at 24 h).
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Animal Model:Male Sprague-Dawley rats (200-220 g) treated with LPS[2]
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Dosage:10 mg/kg
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Administration:Intraperitoneal injection (i.p.); 2 hours after the LPS injection
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Result:Alleviated the histological changes in lung tissues induced by LPS, including severe inflammatory cell infiltration, thickened alveolar walls, obvious diffuse edema, narrowed alveolar space, strengthened interstitial congestion, serious pathological damage and higher scores.
Reduced BALF cells and neutrophils increased by LPS.
Attenuated the wet/dry weight ratio, capillary permeability and d myeloperoxidase (MPO) activity facilitated by LPS.
Chemical Information
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CAS No. 487-58-1
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Appearance Solid
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분자량 246.31
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화학식 C14H18N2O2
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Color White to off-white
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SMILES
C[N+](C)(C)[C@H](C([O-])=O)CC1=CNC2=CC=CC=C12
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Structure Classification
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Initial Source
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Kaohsiung J Med Sci
Hypaphorine exerts anti-inflammatory effects in sepsis induced acute lung injury via modulating DUSP1/p38/JNK pathway. [Abstract]2021 Oct;37(10):883-893. PMID: 34250720 -
용액&용해도
H2O : 100 mg/mL (405.99 mM; Need ultrasonic)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (405.99 mM); Clear solution; Need ultrasonic
순도&문서
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Data Sheet (286 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
[1]. Keller BO, et al. Hypaphorine is present in human milk in association with consumption of legumes. J Agric Food Chem. 2013 Aug 7;61(31):7654-60. [Content Brief]
[2]. Ding YH, et al. Hypaphorine exerts anti-inflammatory effects in sepsis induced acute lung injury via modulating DUSP1/p38/JNK pathway. Kaohsiung J Med Sci. 2021 Oct;37(10):883-893. [Content Brief]
[3]. Ditengou FA, et al. Hypaphorine from the ectomycorrhizal fungus Pisolithus tinctorius counteracts activities of indole-3-acetic acid and ethylene but not synthetic auxins in eucalypt seedlings. Mol Plant Microbe Interact. 2000 Feb;13(2):151-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 4.0599 mL | 20.2996 mL | 40.5992 mL | 101.4981 mL |
| 5 mM | 0.8120 mL | 4.0599 mL | 8.1198 mL | 20.2996 mL | |
| 10 mM | 0.4060 mL | 2.0300 mL | 4.0599 mL | 10.1498 mL | |
| 15 mM | 0.2707 mL | 1.3533 mL | 2.7066 mL | 6.7665 mL | |
| 20 mM | 0.2030 mL | 1.0150 mL | 2.0300 mL | 5.0749 mL | |
| 25 mM | 0.1624 mL | 0.8120 mL | 1.6240 mL | 4.0599 mL | |
| 30 mM | 0.1353 mL | 0.6767 mL | 1.3533 mL | 3.3833 mL | |
| 40 mM | 0.1015 mL | 0.5075 mL | 1.0150 mL | 2.5375 mL | |
| 50 mM | 0.0812 mL | 0.4060 mL | 0.8120 mL | 2.0300 mL | |
| 60 mM | 0.0677 mL | 0.3383 mL | 0.6767 mL | 1.6916 mL | |
| 80 mM | 0.0507 mL | 0.2537 mL | 0.5075 mL | 1.2687 mL | |
| 100 mM | 0.0406 mL | 0.2030 mL | 0.4060 mL | 1.0150 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Hypaphorine Related Classifications
HY-N2179 Related Classifications
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