Pulixin
Pulixin prevents FREP1 from binding to P. falciparum-infected cell lysate. Pulixin blocks the transmission of the parasite to mosquitoes with an EC50 of 11 µM. Pulixin also inhibits the proliferation of asexual-stage P. falciparum with an EC50 of 47 nM.
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- CAS No.: 2826264-81-5
- 화학식: C14H11NO4
- 분자량:257.24
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
IC50 & Target
[1]|
Plasmodium |
Microbial Metabolite |
In Vitro
Pulixin, a fungal metabolite, inhibits Plasmodium falciparum transmission and infection. It does not show cytotoxic effects at a concentration of 116 µM or lower[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 2826264-81-5
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분자량 257.24
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화학식 C14H11NO4
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SMILES
O=C1C2=C(O)C=C(O)C=C2C3=C(O1)C=C(N)C=C3C
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Structure Classification
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Initial Source
Purpureocillium lilacinum
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocol
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Detergent-Based Total Protein Extraction from Cultured Mammalian Cells (RIPA/NP-40/Triton Lysis)
Detergent-based total protein extraction lyses cultured mammalian cells by using amphipathic detergents to disrupt lipid membranes and solubilize proteins into aqueous lysate; RIPA combines non-ionic detergent with ionic detergents, whereas NP-40/Triton buffers are milder non-ionic lysis conditions. The readout is the recovered clarified protein lysate, which is commonly quantified by detergent-compatible protein assays and then used for downstream protein analysis such as SDS-PAGE and Western blotting.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
순도&문서
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pulixin Related Classifications
HY-N10197 Related Classifications
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