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Methotrexate hydrate

(Synonyms: Amethopterin hydrate; CL14377 hydrate; WR19039 hydrate)
80 Cited Publications
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Based on 80 publication(s) in Google Scholar

Methotrexate hydrate (Amethopterin hydrate; CL14377 hydrate; WR19039 hydrate) is an orally active antifolate (Antifolate). Methotrexate hydrate inhibits dihydrofolate reductase (DHFR), blocks tetrahydrofolate production, suppresses purine/pyrimidine synthesis and transmethylation, and causes intracellular accumulation of AICAR. Methotrexate hydrate promotes extracellular adenosine release, regulates the cytokine network, and inhibits the alarmin function of HMGB1. Methotrexate hydrate induces apoptosis and cytotoxicity, upregulates the expression of iNOS and COX-2, suppresses hippocampal neurogenesis, and induces pulmonary fibrosis. Methotrexate hydrate is used in the research of various immune and inflammation-related diseases such as arthritis, psoriasis, systemic lupus erythematosus, as well as pulmonary fibrosis and breast cancer.

For research use only. We do not sell to patients.

  • Purity: 99.66%
  • CAS No.: 133073-73-1
  • Formula: C20H22N8O5.xH2O
  • Storage:

    4°C, sealed storage, away from moisture and light

    * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

  • Methotrexate hydrate Chemical Structure
    Methotrexate hydrate Chemical Structure
  • Experimental Validation Results for Methotrexate hydrate
  • Experimental Validation Results for Methotrexate hydrate
  • Experimental Validation Results for Methotrexate hydrate
  • Experimental Validation Results for Methotrexate hydrate
  • Experimental Validation Results for Methotrexate hydrate
CAS No.: 133073-73-1
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Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
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Cell Imaging/StainingCell Proliferation/Viability AssayRT-PCRCell Migration/Invasion Assay
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  • RT-PCR
  • Cell Proliferation/Viability Assay
  • Cell Migration/Invasion Assay
  • Cell Proliferation/Viability Assay

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  • Methotrexate hydrate Chemical Structure
    Methotrexate hydrate Chemical Structure
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    Methotrexate hydrate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 28;16(1):2071. [Abstract]

    Customer Validation
     OIS cells were infected with shCtrl orshACSS2 followed by selection and treated with or without MTX (40 μM) for 2 days. Cells were stained with γH2AX.

  • 1/16

    Methotrexate hydrate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 28;16(1):2071. [Abstract]

    Customer Validation
     OIS cells were infected with shCtrl orshACSS2 followed by selection and treated with or without MTX (40 μM) for 2 days. The expression of SASP genes was analysed by qRT–PCR.

  • 1/16

    Methotrexate hydrate purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Aug:74:609-620. [Abstract]

    Customer Validation
     The cell viability of MH7A cells treated with methotrexate (1 μM) for 24 h was determined by the CCK-8 method.

  • 1/16

    Methotrexate hydrate purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Aug:74:609-620. [Abstract]

    Customer Validation
     The cell migration of MH7A cells treated with methotrexate (1 μM) for 24 h.

  • 1/16

    Methotrexate hydrate purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2023 Jul 3;133(13):e169993. [Abstract]

    Customer Validation
     A CellTiter-Glo® luminescent cell viability assay was performed on CAL51 WT and MLL4 KO cells, which were treated with 0, 0.04, or 0.4 μM Methotrexate (MTX) in the presence of H2O, 50 μM thymidine, or 50 μM inosine.

  • Methotrexate hydrate