2. Cell Cycle/DNA Damage Antibody-drug Conjugate/ADC Related Apoptosis Anti-infection
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  4. Methotrexate disodium

Methotrexate disodium  (Synonyms: Amethopterin disodium; CL14377 disodium; WR19039 disodium)

Cat. No.: HY-14519A Purity: 99.63%
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Methotrexate (Amethopterin) disodium, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis. Methotrexate disodium, also an immunosuppressant and antineoplastic agent, is used for the research of rheumatoid arthritis and a number of different cancers (such as acute lymphoblastic leukemia).

For research use only. We do not sell to patients.

CAS No. : 7413-34-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in Water In-stock
Solid
100 mg In-stock
500 mg In-stock
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5 g   Get quote  

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Customer Review

Based on 76 publication(s) in Google Scholar

Other Forms of Methotrexate disodium:

Methotrexate disodium Related Antibodies

Top Publications Citing Use of Products

76 Publications Citing Use of MCE Methotrexate disodium

Cell Imaging/Staining
Cell Proliferation/Viability Assay
RT-PCR
Cell Migration/Invasion Assay

    Methotrexate disodium purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 28;16(1):2071.  [Abstract]

    OIS cells were infected with shCtrl orshACSS2 followed by selection and treated with or without MTX (40 μM) for 2 days. Cells were stained with γH2AX.

    Methotrexate disodium purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 28;16(1):2071.  [Abstract]

    OIS cells were infected with shCtrl orshACSS2 followed by selection and treated with or without MTX (40 μM) for 2 days. The expression of SASP genes was analysed by qRT–PCR.

    Methotrexate disodium purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Aug:74:609-620.  [Abstract]

    The cell viability of MH7A cells treated with methotrexate (1 μM) for 24 h was determined by the CCK-8 method.

    Methotrexate disodium purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Aug:74:609-620.  [Abstract]

    The cell migration of MH7A cells treated with methotrexate (1 μM) for 24 h.

    Methotrexate disodium purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2023 Jul 3;133(13):e169993.  [Abstract]

    A CellTiter-Glo® luminescent cell viability assay was performed on CAL51 WT and MLL4 KO cells, which were treated with 0, 0.04, or 0.4 μM Methotrexate (MTX) in the presence of H2O, 50 μM thymidine, or 50 μM inosine.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Methotrexate (Amethopterin) disodium, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis. Methotrexate disodium, also an immunosuppressant and antineoplastic agent, is used for the research of rheumatoid arthritis and a number of different cancers (such as acute lymphoblastic leukemia)[1][2][3].

    IC50 & Target

    Traditional Cytotoxic Agents

     

    In Vivo

    Methotrexate (Amethopterin) disodium reduces thymus and spleen indices of mice. Methotrexate disodium markedly decreases white blood cells, thymic and splenic lymphocytes at dose ≥5 mg/kg. However, there is a significant difference between the treatment plus control group and the model group (p<0.01). The combination of grape seed proanthocyanidins and Siberian ginseng eleutherosides obviously diminishes the effects of Methotrexate exposure on indices of thymus and spleens in mice[2].
    Methotrexate (MTX) disodium (2 mg/kg; i.p.; once in a week for 5 weeks) is effective in Freund's complete adjuvant-induced arthritis. The combination of Methotrexate disodium (1 mg/kg; i.p.; once in a week for 5 weeks) and Curcumin (30 mg/kg and 100 mg/kg, thrice a week for 5 weeks; i.p.) shows a significant anti-arthritic action and protection from hematological toxicity[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    498.40

    Formula

    C20H20N8Na2O5
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    NC1=NC(N)=C2C(N=CC(CN(C)C3=CC=C(C(N[C@@H](CCC(O[Na])=O)C(O[Na])=O)=O)C=C3)=N2)=N1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    H2O : 50 mg/mL (100.32 mM; Need ultrasonic)

    DMSO : 5 mg/mL (10.03 mM; ultrasonic and warming and adjust pH to 5 with HCl and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.0064 mL 10.0321 mL 20.0642 mL
    5 mM 0.4013 mL 2.0064 mL 4.0128 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

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    Dosing volume
    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 2.0064 mL 10.0321 mL 20.0642 mL 50.1605 mL
    5 mM 0.4013 mL 2.0064 mL 4.0128 mL 10.0321 mL
    10 mM 0.2006 mL 1.0032 mL 2.0064 mL 5.0161 mL
    H2O 15 mM 0.1338 mL 0.6688 mL 1.3376 mL 3.3440 mL
    20 mM 0.1003 mL 0.5016 mL 1.0032 mL 2.5080 mL
    25 mM 0.0803 mL 0.4013 mL 0.8026 mL 2.0064 mL
    30 mM 0.0669 mL 0.3344 mL 0.6688 mL 1.6720 mL
    40 mM 0.0502 mL 0.2508 mL 0.5016 mL 1.2540 mL
    50 mM 0.0401 mL 0.2006 mL 0.4013 mL 1.0032 mL
    60 mM 0.0334 mL 0.1672 mL 0.3344 mL 0.8360 mL
    80 mM 0.0251 mL 0.1254 mL 0.2508 mL 0.6270 mL
    100 mM 0.0201 mL 0.1003 mL 0.2006 mL 0.5016 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Methotrexate disodium Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Methotrexate disodium7413-34-5Amethopterin disodium CL14377 disodium WR19039 disodiumAntifolateDNA/RNA SynthesisADC PayloadApoptosisBacterialADC CytotoxinreductaseDNAsynthesisimmunosuppressantantineoplasticrheumatoidarthritislymphoblasticleukemiaInhibitorinhibitorinhibit

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