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  3. Methotrexate disodium

Methotrexate disodium  (Synonyms: Amethopterin disodium; CL14377 disodium; WR19039 disodium)

Cat. No.: HY-14519A Purity: 99.71%
COA Handling Instructions

Methotrexate (Amethopterin) disodium, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis. Methotrexate disodium, also an immunosuppressant and antineoplastic agent, is used for the research of rheumatoid arthritis and a number of different cancers (such as acute lymphoblastic leukemia).

For research use only. We do not sell to patients.

Methotrexate disodium Chemical Structure

Methotrexate disodium Chemical Structure

CAS No. : 7413-34-5

Size Price Stock Quantity
Solution
10 mM * 1 mL in Water USD 66 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 66 In-stock
Solid
100 mg USD 60 In-stock
500 mg USD 156 In-stock
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5 g   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 25 publication(s) in Google Scholar

Other Forms of Methotrexate disodium:

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Description

Methotrexate (Amethopterin) disodium, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis. Methotrexate disodium, also an immunosuppressant and antineoplastic agent, is used for the research of rheumatoid arthritis and a number of different cancers (such as acute lymphoblastic leukemia)[1][2][3].

IC50 & Target

Traditional Cytotoxic Agents

 

In Vivo

Methotrexate (Amethopterin) disodium reduces thymus and spleen indices of mice. Methotrexate disodium markedly decreases white blood cells, thymic and splenic lymphocytes at dose ≥5 mg/kg. However, there is a significant difference between the treatment plus control group and the model group (p<0.01). The combination of grape seed proanthocyanidins and Siberian ginseng eleutherosides obviously diminishes the effects of Methotrexate exposure on indices of thymus and spleens in mice[2].
Methotrexate (MTX) disodium (2 mg/kg; i.p.; once in a week for 5 weeks) is effective in Freund's complete adjuvant-induced arthritis. The combination of Methotrexate disodium (1 mg/kg; i.p.; once in a week for 5 weeks) and Curcumin (30 mg/kg and 100 mg/kg, thrice a week for 5 weeks; i.p.) shows a significant anti-arthritic action and protection from hematological toxicity[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial
Molecular Weight

498.40

Appearance

Solid

Formula

C20H20N8Na2O5

CAS No.
SMILES

NC1=NC(N)=C2C(N=CC(CN(C)C3=CC=C(C(N[C@@H](CCC(O[Na])=O)C(O[Na])=O)=O)C=C3)=N2)=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (100.32 mM; Need ultrasonic)

DMSO : 5 mg/mL (10.03 mM; ultrasonic and warming and adjust pH to 5 with HCl and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0064 mL 10.0321 mL 20.0642 mL
5 mM 0.4013 mL 2.0064 mL 4.0128 mL
10 mM 0.2006 mL 1.0032 mL 2.0064 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Methotrexate disodium
Cat. No.:
HY-14519A
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