2. Cell Cycle/DNA Damage Antibody-drug Conjugate/ADC Related Apoptosis Anti-infection
  3. Antifolate DNA/RNA Synthesis ADC Payload Apoptosis Bacterial
  4. Methotrexate monohydrate

Methotrexate monohydrate  (Synonyms: Amethopterin monohydrate; CL14377 monohydrate; WR19039 monohydrate)

Cat. No.: HY-14519D
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Methotrexate (Amethopterin) monohydrate, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis. Methotrexate monohydrate, also an immunosuppressant and antineoplastic agent, is used for the research of rheumatoid arthritis and a number of different cancers (such as acute lymphoblastic leukemia).

For research use only. We do not sell to patients.

CAS No. : 6745-93-3

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Methotrexate monohydrate Related Antibodies

Top Publications Citing Use of Products

76 Publications Citing Use of MCE Methotrexate monohydrate

Cell Imaging/Staining
Cell Proliferation/Viability Assay
RT-PCR
Cell Migration/Invasion Assay

    Methotrexate monohydrate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 28;16(1):2071.  [Abstract]

    OIS cells were infected with shCtrl orshACSS2 followed by selection and treated with or without MTX (40 μM) for 2 days. Cells were stained with γH2AX.

    Methotrexate monohydrate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 28;16(1):2071.  [Abstract]

    OIS cells were infected with shCtrl orshACSS2 followed by selection and treated with or without MTX (40 μM) for 2 days. The expression of SASP genes was analysed by qRT–PCR.

    Methotrexate monohydrate purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Aug:74:609-620.  [Abstract]

    The cell viability of MH7A cells treated with methotrexate (1 μM) for 24 h was determined by the CCK-8 method.

    Methotrexate monohydrate purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Aug:74:609-620.  [Abstract]

    The cell migration of MH7A cells treated with methotrexate (1 μM) for 24 h.

    Methotrexate monohydrate purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2023 Jul 3;133(13):e169993.  [Abstract]

    A CellTiter-Glo® luminescent cell viability assay was performed on CAL51 WT and MLL4 KO cells, which were treated with 0, 0.04, or 0.4 μM Methotrexate (MTX) in the presence of H2O, 50 μM thymidine, or 50 μM inosine.

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    Description

    Methotrexate (Amethopterin) monohydrate, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis. Methotrexate monohydrate, also an immunosuppressant and antineoplastic agent, is used for the research of rheumatoid arthritis and a number of different cancers (such as acute lymphoblastic leukemia)[1][2][3].

    IC50 & Target

    Traditional Cytotoxic Agents

     

    In Vivo

    Methotrexate (Amethopterin) monohydrate reduces thymus and spleen indices of mice. Methotrexate monohydrate markedly decreases white blood cells, thymic and splenic lymphocytes at dose ≥5 mg/kg. However, there is a significant difference between the treatment plus control group and the model group (p<0.01). The combination of grape seed proanthocyanidins and Siberian ginseng eleutherosides obviously diminishes the effects of Methotrexate monohydrate exposure on indices of thymus and spleens in mice[2].
    Methotrexate (MTX) monohydrate (2 mg/kg; i.p.; once in a week for 5 weeks) is effective in Freund's complete adjuvant-induced arthritis. The combination of Methotrexate monohydrate (1 mg/kg; i.p.; once in a week for 5 weeks) and Curcumin (30 mg/kg and 100 mg/kg, thrice a week for 5 weeks; i.p.) shows a significant anti-arthritic action and protection from hematological toxicity[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    472.45

    Formula

    C20H24N8O6
    CAS No.
    SMILES

    NC1=NC(N)=C2C(N=CC(CN(C)C3=CC=C(C(N[C@@H](CCC(O)=O)C(O)=O)=O)C=C3)=N2)=N1.O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
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    Methotrexate monohydrate Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Methotrexate6745-93-3AmethopterinCL14377WR19039CL 14377CL-14377WR 19039WR-19039AntifolateDNA/RNA SynthesisADC PayloadApoptosisBacterialADC CytotoxinreductaseDNAsynthesisimmunosuppressantantineoplasticrheumatoidarthritislymphoblasticleukemiaInhibitorinhibitorinhibit

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