2. Protein Tyrosine Kinase/RTK Immunology/Inflammation
  3. FGFR IFNAR Interleukin Related
  4. OM-RCA-01

OM-RCA-01 is an anti-FGFR1 monoclonal antibody with a Kd of 1.59 nM for human FGFR1. OM-RCA-01 inhibits the phosphorylation of FGFR1, blocks FGF-mediated signaling pathways, and suppresses the proliferation of downstream tumor cells. OM-RCA-01 delays tumor growth in lung cancer and renal cancer xenograft models expressing FGFR1. When combined with Nivolumab, OM-RCA-01 enhances the release of IFN-γ and IL-2. OM-RCA-01 is applicable for the research of lung cancer and renal cell carcinoma.

For research use only. We do not sell to patients.

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OM-RCA-01 Related Antibodies

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  • Purity & Documentation

  • References

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Description

OM-RCA-01 is an anti-FGFR1 monoclonal antibody with a Kd of 1.59 nM for human FGFR1. OM-RCA-01 inhibits the phosphorylation of FGFR1, blocks FGF-mediated signaling pathways, and suppresses the proliferation of downstream tumor cells. OM-RCA-01 delays tumor growth in lung cancer and renal cancer xenograft models expressing FGFR1. When combined with Nivolumab, OM-RCA-01 enhances the release of IFN-γ and IL-2. OM-RCA-01 is applicable for the research of lung cancer and renal cell carcinoma[1].

Species Reactivity

Human
In Vitro

OM-RCA-01 binds to FGFR1 with high affinity (Kd = 1.59 nM) and inhibits its in vitro kinase activity[1].
OM-RCA-01 (1-100 μg/mL, 3 h) potently inhibits FGFR1 phosphorylation and cell proliferation in FGFR1-overexpressing human lung cancer cell line A549 (GI50 = 9.69 μg/mL), but exerts no significant effect on FGFR1-low-expressing human melanoma cell line Mel Kor[1].
OM-RCA-01 (0.5-10 nmol/L; 5 days) enhances Nivolumab-induced IFN-γ release and T-cell proliferation in a human allogeneic MLR assay, whereas it exhibits minimal effects when used alone when combined with Nivolumab (HY-P9903)[1].
OM-RCA-01 (1 μg/mL; 4 days) increases Nivolumab-induced IL-2 secretion by 29%-74% in human peripheral blood mononuclear cells (PBMCs) stimulated with Staphylococcal enterotoxin B (SEB) (HY-P1900)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

OM-RCA-01 Related Antibodies

Western Blot Analysis[1]

Cell Line: A549 cells
Concentration: 1-100μg/mL
Incubation Time: 3 h
Result: Resulted in a dose-dependent
suppression of FGFR1 phosphorylation.
Parmacokinetics
Species Dose Route C0 T1/2 Vd CL AUC0-t AUC0-∞ MRT
Rat[1] 10 mg/kg i.v. 186 ng/mL 121 h 532 L 3.05 L/h 10665 ng·h/mL 32818 ng·h/mL 34 h
Rat[1] 30 mg/kg i.v. 177 ng/mL 136 h 2533 L 12.88 L/h 14237 ng·h/mL 46602 ng·h/mL 34 h
Rat[1] 100 mg/kg i.v. 245 ng/mL 234 h 7569 L 22.44 L/h 16383 ng·h/mL 89131 ng·h/mL 35 h
In Vivo

OM-RCA-01 (30 mg/kg; i.v.; twice weekly) induces a statistically significant two-fold reduction in median tumor volume and delays tumor growth in A549 lung cancer xenograft models, and combination with Pembrolizumab (HY-P9902) achieves 89% tumor growth inhibition[1].
OM-RCA-01 (30 mg/kg; once every 3 days) achieves 96% tumor growth delay in the cancer-associated fibroblast (CAF)-positive renal cell carcinoma cohort and 75% tumor growth delay in the CAF-negative renal cell carcinoma cohort, while increasing IFN-γ and IL-2 cytokine levels[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: NCr nu/nu mice (female、6-12 weeks)[1]
Dosage: 30 mg/kg
Administration: i.v.; twice weekly
Result: Resulted in significant suppression of tumor growth.
Reduced median tumor volume Pembrolizumab alone.
Caused tumor growth to plateau with minimal growth from day 16 onwards.
Resulted in no deaths or body weight loss in the combination group.
Animal Model: Charles River mice[1]
Dosage: 30 mg/kg
Administration: every 3 days
Result: Achieved 96% tumor growth delay (TGD) in CAF-positive cohorts and 75% TGD in CAF-negative cohorts, both statistically significant.
Increased IFN-γ release by 43% and IL-2 secretion by 65% compared to the control IgG group.
Gene ID

2260  [NCBI]

Accession
Target

FGFR1/CD331
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

[OM-RCA-01]
Shipping

Shipping with dry ice.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
  • Immobilized Human FGFR1/CD331 Protein, His Tag can bind OM-RCA-01. The EC50 for this effect is 121.7 ng/mL.
Purity & Documentation
References
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OM-RCA-01 Related Classifications

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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× = ×
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

OM-RCA-01FGFRIFNARInterleukin RelatedFibroblast growth factor receptorInterferon-α/β receptorInterferon-alpha/beta receptor ILanti-FGFR1 monoclonal antibodyA549 cellsNCr nu/nu miceCharles River micelung cancerrenal cell carcinoma
Inhibitorinhibitorinhibit

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OM-RCA-01
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HY-P991999
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