OM-RCA-01
OM-RCA-01 is an anti-FGFR1 monoclonal antibody with a Kd of 1.59 nM for human FGFR1. OM-RCA-01 inhibits the phosphorylation of FGFR1, blocks FGF-mediated signaling pathways, and suppresses the proliferation of downstream tumor cells. OM-RCA-01 delays tumor growth in lung cancer and renal cancer xenograft models expressing FGFR1. When combined with Nivolumab, OM-RCA-01 enhances the release of IFN-γ and IL-2. OM-RCA-01 is applicable for the research of lung cancer and renal cell carcinoma.
For research use only. We do not sell to patients.
- Purity: ≥99.0%
- Molecular Weight:144.85 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Human IgG1 kappa
Human
OM-RCA-01 binds to FGFR1 with high affinity (Kd = 1.59 nM) and inhibits its in vitro kinase activity[1].
OM-RCA-01 (1-100 μg/mL, 3 h) potently inhibits FGFR1 phosphorylation and cell proliferation in FGFR1-overexpressing human lung cancer cell line A549 (GI50 = 9.69 μg/mL), but exerts no significant effect on FGFR1-low-expressing human melanoma cell line Mel Kor[1].
OM-RCA-01 (0.5-10 nmol/L; 5 days) enhances Nivolumab-induced IFN-γ release and T-cell proliferation in a human allogeneic MLR assay, whereas it exhibits minimal effects when used alone when combined with Nivolumab (HY-P9903)[1].
OM-RCA-01 (1 μg/mL; 4 days) increases Nivolumab-induced IL-2 secretion by 29%-74% in human peripheral blood mononuclear cells (PBMCs) stimulated with Staphylococcal enterotoxin B (SEB) (HY-P1900)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549 cells
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Concentration:1-100μg/mL
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Incubation Time:3 h
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Result:Resulted in a dose-dependent
suppression of FGFR1 phosphorylation.
| Species | Dose | Route | C0 | T1/2 | Vd | CL | AUC0-t | AUC0-∞ | MRT |
|---|---|---|---|---|---|---|---|---|---|
| Rat[1] | 10 mg/kg | i.v. | 186 ng/mL | 121 h | 532 L | 3.05 L/h | 10665 ng·h/mL | 32818 ng·h/mL | 34 h |
| Rat[1] | 30 mg/kg | i.v. | 177 ng/mL | 136 h | 2533 L | 12.88 L/h | 14237 ng·h/mL | 46602 ng·h/mL | 34 h |
| Rat[1] | 100 mg/kg | i.v. | 245 ng/mL | 234 h | 7569 L | 22.44 L/h | 16383 ng·h/mL | 89131 ng·h/mL | 35 h |
OM-RCA-01 (30 mg/kg; once every 3 days) achieves 96% tumor growth delay in the cancer-associated fibroblast (CAF)-positive renal cell carcinoma cohort and 75% tumor growth delay in the CAF-negative renal cell carcinoma cohort, while increasing IFN-γ and IL-2 cytokine levels[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:NCr nu/nu mice (female、6-12 weeks)[1]
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Dosage:30 mg/kg
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Administration:i.v.; twice weekly
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Result:Resulted in significant suppression of tumor growth.
Reduced median tumor volume Pembrolizumab alone.
Caused tumor growth to plateau with minimal growth from day 16 onwards.
Resulted in no deaths or body weight loss in the combination group.
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Animal Model:Charles River mice[1]
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Dosage:30 mg/kg
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Administration:every 3 days
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Result:Achieved 96% tumor growth delay (TGD) in CAF-positive cohorts and 75% TGD in CAF-negative cohorts, both statistically significant.
Increased IFN-γ release by 43% and IL-2 secretion by 65% compared to the control IgG group.
FGFR1/CD331
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
ELISA, FACS, Functional assay
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Immobilized Human FGFR1/CD331 Protein, His Tag can bind OM-RCA-01. The EC50 for this effect is 121.7 ng/mL.
Chemical Information
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Appearance Liquid
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Molecular Weight 144.85 kDa
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Color Colorless to light yellow
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SMILES
[OM-RCA-01]
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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