Ophiobolin C
Ophiobolin C inhibits CCR5 binding to the envelop protein gp120 and CD4, which is responsible for mediating the entry of HIV-1 into cells. Ophiobolin C is also cytotoxic to chronic lymphocytic leukemia cells.
For research use only. We do not sell to patients.
- CAS No.: 19022-51-6
- Formula: C25H38O3
- Molecular Weight:386.57
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
-
HY-13782
Tenofovir Disoproxil fumarateNucleotide Reverse Transcriptase Inhibitor -
HY-17583
GriseofulvinAntifungal Antibiotic -
HY-13782R
Tenofovir Disoproxil fumarate (Standard)Nucleotide Reverse Transcriptase Inhibitor -
HY-17583R
Griseofulvin (Standard) -
HY-138800
SpinosadAcetylcholine Agonist -
HY-125060
ViridicatinFungal Metabolite -
HY-126638
Marcfortine ANematode
Biological Activity
Description
IC50 & Target
|
CCR5 |
HIV-1 |
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| CHO | IC50 |
40 μM
Compound: 2
|
Displacement of [125I]gp-120 from human CCR5 receptor expressed in CHO cells
Displacement of [125I]gp-120 from human CCR5 receptor expressed in CHO cells
|
[PMID: 15217290] |
Chemical Information
-
CAS No. 19022-51-6
-
Molecular Weight 386.57
-
Formula C25H38O3
-
SMILES
O=C/C1=C/C[C@]([C@@H]([C@@H](C)CC/C=C(C)\C)CC2)([H])[C@@]2(C)C[C@@]([C@](C)(O)C3)([H])[C@]1([H])C3=O
-
Synonyms
Zizanin A
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
-
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
[1]. Jayasuriya H, et al. Isolation and structure of antagonists of chemokine receptor (CCR5). J Nat Prod. 2004 Jun;67(6):1036-8. [Content Brief]
[2]. Bladt TT, et al. Bio-activity and dereplication-based discovery of ophiobolins and other fungal secondary metabolites targeting leukemia cells. Molecules. 2013 Nov 26;18(12):14629-50. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Ophiobolin C Related Classifications
HY-123902 Related Classifications
Powered by Bioz