Periplanetasin-4
Periplanetasin-4 is an antimicrobial peptide that can be derived from the American cockroach (Periplaneta americana). Periplanetasin-4 reduces cell rounding and apoptosis. Periplanetasin-4 blocks Clostridium difficile toxin A-induced ROS production and the activation of downstream p38 MAPK and p21. Periplanetasin-4 significantly increases mitochondrial calcium level, reduces DPH fluorescence intensity and vacuolar dysfunction in Candida albicans ATCC 90028 cells. Periplanetasin-4 significantly ameliorates toxin A-induced mucosal damage in the mouse gut. Periplanetasin-4 can be used for the study of colitis.
For research use only. We do not sell to patients.
- CAS No.: 2206807-06-7
- Formula: C70H110N20O15S
- Molecular Weight:1503.81
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Periplanetasin-4 (100 μg/mL, 48 h) significantly rescues the loss of cell viability and reduces cell rounding induced by Clostridium difficile toxin A in HT29 cells[1].
Periplanetasin-4 (100 μg/mL, 48 h) markedly decreases the number of TUNEL-positive apoptotic HT29 cells and inhibits the activation of caspase-3 (a hallmark of apoptosis) induced by Clostridium difficile toxin A[1].
Periplanetasin-4 (100 μg/mL, 45 min) completely inhibits the increase in ROS production induced by Clostridium difficile toxin A in HT29 cells[1].
Periplanetasin-4 (100 μg/mL, 4 h) significantly blocks the activation of p38MAPK and the up-regulation of p21 in HT29 cells[1].
Periplanetasin-4 (5 μM, 2-4 h) significantly increases PI influx and DiBAC4(3) accumulation in Candida albicans ATCC 90028 cells[2].
Periplanetasin-4 (5 μM, 2 h) significantly elevates intracellular ROS level, increases mitochondrial superoxide radical and hydrogen peroxide production in Candida albicans ATCC 90028 cells[2].
Periplanetasin-4 (5 μM, 2 h) significantly induces phosphatidylserine externalization and caspase activation in Candida albicans ATCC 90028 protoplasts[2].
Periplanetasin-4 (5 μM, 2 h) significantly increases malondialdehyde (MDA) level, enhances catalase activity and total glutathione content, causes mitochondrial membrane potential collapse, increases mitochondrial biomass, and promotes mitochondrial fragmentation in Candida albicans ATCC 90028 cells[2].
Periplanetasin-4 (5 μM, 2 h) significantly increases BCECF and C-DCFDA fluorescence intensity and mitochondrial calcium level, reduces DPH fluorescence intensity and vacuolar dysfunction in Candida albicans ATCC 90028 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Clostridium difficile toxin A induced HT29 cells
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Concentration:100 μg/mL
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Incubation Time:48 h
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Result:Decreased the number of TUNEL-positive apoptotic HT29 cells.
Inhibited the activation of caspase-3.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Clostridium difficile toxin A-induced mouse enteritis model: 6-week-old mice were anesthetized by intraperitoneal injection of sodium pentobarbital (50 mg/kg)[1]
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Dosage:100 μg/mL co-administered with 3 nM Clostridium difficile toxin A
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Administration:Intraluminal injection into mouse ileal loops for 4 h
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Result:Ameliorated toxin A-induced mucosal damage in the mouse gut.
Reduced the elevation of interleukin-6 (IL-6) production in the ileal loop tissues of mice.
Chemical Information
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CAS No. 2206807-06-7
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Molecular Weight 1503.81
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Formula C70H110N20O15S
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Sequence
Leu-Arg-His-Lys-Val-Tyr-Gly-Tyr-Cys-Val-Leu-Gly-Pro-NH2
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Sequence Shortening
LRHKVYGYCVLGP-NH2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Yoon IN, et al. The American cockroach peptide periplanetasin-4 inhibits Clostridium difficile toxin A-induced cell toxicities and inflammatory responses in the mouse gut. J Pept Sci. 2017 Nov;23(11):833-839. [Content Brief]
[2]. Lee H, et al. Periplanetasin-4, a novel antimicrobial peptide from the cockroach, inhibits communications between mitochondria and vacuoles. Biochem J. 2019 Apr 26;476(8):1267-1284. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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