PI3K/mTOR-IN-23
PI3K/mTOR-IN-23 is a dual inhibitor of PI3K and mTOR with IC50 values of 49 and 41 nM. PI3K/mTOR-IN-23 inhibits cancer cells proliferation.
For research use only. We do not sell to patients.
- Purity : 99.0%
- CAS No.: 371943-05-4
- Formula: C16H15N3O2S
- Molecular Weight:313.37
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
mTOR 49 nM (IC50) |
PI3Kα 41 nM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A2780 | IC50 |
0.27 μM
Compound: 1
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Antiproliferative activity against human A2780 cells with PIK3CA and PTEN mutation after 4 days by alamar blue assay
Antiproliferative activity against human A2780 cells with PIK3CA and PTEN mutation after 4 days by alamar blue assay
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[PMID: 18754654] |
| LNCaP | IC50 |
1300 nM
Compound: 1
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Cytotoxicity against human LNCAP cells
Cytotoxicity against human LNCAP cells
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[PMID: 19897362] |
| Sf9 | IC50 |
0.008 μM
Compound: 1
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Inhibition of human recombinant PI3K p110alpha expressed in Sf9 cells coexpressing p85alpha by radiometric scintillation proximity assay
Inhibition of human recombinant PI3K p110alpha expressed in Sf9 cells coexpressing p85alpha by radiometric scintillation proximity assay
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[PMID: 18754654] |
| U-87MG ATCC | IC50 |
1.1 μM
Compound: 1
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Antiproliferative activity against PTEN-null human U87MG cells after 4 days by alamar blue assay
Antiproliferative activity against PTEN-null human U87MG cells after 4 days by alamar blue assay
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[PMID: 18754654] |
In Vitro
PI3K/mTOR-IN-23 (Compound 1) acts as a dual inhibitor of mTOR and PI3K-α in recombinant enzyme assays, with an mTOR IC50 of 49 nM, a PI3K-α IC50 of 41 nM[1].
PI3K/mTOR-IN-23 inhibits LNCaP cell proliferation with an IC50 of 1300 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 371943-05-4
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Appearance Solid
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Molecular Weight 313.37
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Formula C16H15N3O2S
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Color White to off-white
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SMILES
OC1=CC(C2=NC3=C(C(N4CCOCC4)=N2)SC=C3)=CC=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 25 mg/mL (79.78 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.1911 mL | 15.9556 mL | 31.9112 mL | 79.7779 mL |
| 5 mM | 0.6382 mL | 3.1911 mL | 6.3822 mL | 15.9556 mL | |
| 10 mM | 0.3191 mL | 1.5956 mL | 3.1911 mL | 7.9778 mL | |
| 15 mM | 0.2127 mL | 1.0637 mL | 2.1274 mL | 5.3185 mL | |
| 20 mM | 0.1596 mL | 0.7978 mL | 1.5956 mL | 3.9889 mL | |
| 25 mM | 0.1276 mL | 0.6382 mL | 1.2764 mL | 3.1911 mL | |
| 30 mM | 0.1064 mL | 0.5319 mL | 1.0637 mL | 2.6593 mL | |
| 40 mM | 0.0798 mL | 0.3989 mL | 0.7978 mL | 1.9944 mL | |
| 50 mM | 0.0638 mL | 0.3191 mL | 0.6382 mL | 1.5956 mL | |
| 60 mM | 0.0532 mL | 0.2659 mL | 0.5319 mL | 1.3296 mL |
PI3K/mTOR-IN-23 Related Classifications
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