PLSRTLSVAAK 

PLSRTLSVAAK is a peptide substrate used for the detection of PKC phosphorylation activity, and can be applied to in vitro kinase activity assays of PKCδ^[1][2].

PLSRTLSVAAK (0.4 μg/μl; 30 min at 30°C) incubation of membrane-bound PKC preparations from BALB/c mouse hippocampal slices results in measurable substrate phosphorylation, while identical incubation with preparations from C57BL/6N mouse hippocampal slices shows no detectable phosphorylation^[1].
PLSRTLSVAAK kinase-active c-Abl activates GST-PKCδ, which then increases phosphorylation of the PLSRTLSVAAK peptide substrate by a mean fold-increase of ~5 relative to PKCδ alone^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1142.35

C₅₀H₉₁N₁₅O₁₅

105802-83-3

Pro-Leu-Ser-Arg-Thr-Leu-Ser-Val-Ala-Ala-Lys

PLSRTLSVAAK

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Blank T, Nijholt I, Grammatopoulos D K, et al. Corticotropin-releasing factor receptors couple to multiple G-proteins to activate diverse intracellular signaling pathways in mouse hippocampus: role in neuronal excitability and associative learning[J]. Journal of Neuroscience, 2003, 23(2): 700-707.

  [2]. Yuan Z M, Utsugisawa T, Ishiko T, et al. Activation of protein kinase C δ by the c-Abl tyrosine kinase in response to ionizing radiation[J]. Oncogene, 1998, 16