Cat. No.: HY-N1346

Robinin

5 Cited Publications

Customer Review

Based on 5 publication(s) in Google Scholar

Robinin is a flavonoid that can be extracted from the leaves of purple cowpea, inhibiting TGF-β, TLR4/NF-κB and TLR2-PI3k-AKT signaling pathways. Robinin exerts anti-inflammatory and anti-tumor effects. The combination of Robinin and Methotrexate (HY-14519) reduces inflammation in experimental arthritis, Robinin can decrease the Doxorubicin (HY-15142A) induced cardiac toxicity effect.

For research use only. We do not sell to patients.

Purity: 99.96%
CAS No.: 301-19-9
Formula: C₃₃H₄₀O₁₉
Molecular Weight:740.66

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Robinin Chemical Structure

CAS No.: 301-19-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (5)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

TLR2

TLR4

All Toll-like Receptor (TLR) Isoforms :

In Vitro

Robinin (1-10 μg/ml, 24 h) shows no obvious cytotoxicity in hPBMCs cells^[1].
Robinin (6 μg/mL, 24 h) inhibits the expression of MCP1, TNF-α, IL-6 and ICAM-1 proteins and the mRNA levels of TLR2 and TLR4 in hPBMCs cells, and had anti-inflammatory effects ^[1].
Robinin (1 μM, 24 h) inhibits the proliferation and migration of Mia-PACA2 and PANC-1 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

hPBMCs
Concentration:

6 μg/mL
Incubation Time:

24 h
Result:

Inhibited the upregulated expression of MCP 1, TNF-α, IL-6 and ICAM-1 with anti-inflammatory properties.

Cell Viability Assay^[1]

Cell Line:

hPBMCs
Concentration:

1 μg/mL, 2 μg/mL,4 μg/mL,6 μg/mL,8 μg/mL,10 μg/mL
Incubation Time:

24 h
Result:

Concentration from 1 μg/ml-10 μg/ml on hPBMCs had no obvious cytotoxicity.

RT-PCR^[1]

Cell Line:

hPBMCs
Concentration:

1 μg/mL, 2 μg/mL,4 μg/mL,6 μg/mL,8 μg/mL,10 μg/mL
Incubation Time:

24 h
Result:

Inhibited the upregulated expression of TLR2 and TLR4.

Cell Proliferation Assay^[2]

Cell Line:

Mia-PACA2 and PANC-1
Concentration:

1 μM
Incubation Time:

24 h
Result:

Inhibited cell proliferation.

Cell Migration Assay ^[2]

Cell Line:

Mia-PACA2 and PANC-1
Concentration:

1 μM
Incubation Time:

24 h
Result:

Decreased the cell migration area

In Vivo

The combination of Robinin and methotrexate (50 mg/kg and 0.3 mg/kg, Oral gavage, Twice a week) has anti-inflammatory and anti-arthritic action in adjuvant arthritis mice models^[3].
Robinin (50 mg/kg, Oral gavage, 10 days) can modulate the cardioprotective effect of the TGF-β1 signaling pathway on Adriamycin-induced cardiotoxicity^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Adjuvant arthritis mice model^[3]
Dosage:

50 mg/kg Robinin and 0.3 mg/kg MTX twice a week
Administration:

Oral gavage (p.o.)
Result:

Had anti-inflammatory and anti-arthritic action.

Animal Model:

Sprague Dawley rats model^[4]
Dosage:

50 mg/kg Robinin for 10 days
Administration:

Oral gavage (p.o.)
Result:

Reduced the cardiotoxic effects of Doxorubicin-induced

Chemical Information

CAS No. 301-19-9
Appearance Solid
Molecular Weight 740.66
Formula C₃₃H₄₀O₁₉
Color White to yellow
SMILES

O=C1C(O[C@H]2[C@@H]([C@H]([C@H]([C@@H](CO[C@H]3[C@@H]([C@@H]([C@H]([C@H](C)O3)O)O)O)O2)O)O)O)=C(C4=CC=C(O)C=C4)OC5=CC(O[C@H]6[C@@H]([C@@H]([C@H]([C@H](C)O6)O)O)O)=CC(O)=C15
Structure Classification
- Flavonoids
- Flavonols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (5)

All Cell Proliferation/Viability AssayApoptosis AnalysisWBIFELISA

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Journal Impact Factor

Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530
Toxicol Appl Pharmacol

RGS2 attenuates alveolar macrophage damage by inhibiting the Gq/11-Ca2+ pathway during cowshed PM2.5 exposure, and aberrant RGS2 expression is associated with TLR2/4 activation. [Abstract]

2024 May 20:487:116976. PMID: 38777097
Cell Biochem Biophys

Robinin protects chondrocytes injury via TLR2/TLR4/NF-κB signaling in osteoarthritis. [Abstract]

2025 Mar;83(1):647-656. PMID: 39673685

Robinin purchased from MedChemExpress. Usage Cited in: Cell Biochem Biophys. 2025 Mar;83(1):647-656. [Abstract]

CCK-8 showed the cell viability in the isolated mouse chondrocytes under the treatment of IL-1β and Robinin (2, 4, 6, 8, 10, 12 μg/mL).

Robinin purchased from MedChemExpress. Usage Cited in: Cell Biochem Biophys. 2025 Mar;83(1):647-656. [Abstract]

Flow cytometry showed the cell apoptosis in the isolated mouse chondrocytes under the treatment of IL-1β and Robinin (10 μg/mL).

Robinin purchased from MedChemExpress. Usage Cited in: Cell Biochem Biophys. 2025 Mar;83(1):647-656. [Abstract]

Western blotting evaluated the levels of apoptosis-related proteins in chondrocytes treated with IL-1β and Robinin (10 μg/mL).

Robinin purchased from MedChemExpress. Usage Cited in: Cell Biochem Biophys. 2025 Mar;83(1):647-656. [Abstract]

Immunofluorescence staining in chondrocytes treated with IL-1β and Robinin (10 μg/mL).

Robinin purchased from MedChemExpress. Usage Cited in: Cell Biochem Biophys. 2025 Mar;83(1):647-656. [Abstract]

ELISA showed the concentrations of inflammatory markers in the supernatant of the chondrocytes treated with IL-1β and Robinin (10 μg/mL).
Research Square Preprint

2023 Dec 25.

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (337.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.3501 mL

6.7507 mL

13.5015 mL

5 mM

0.2700 mL

1.3501 mL

2.7003 mL

10 mM

0.1350 mL

0.6751 mL

1.3501 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (2.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (2.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (2.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (288 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Janeesh PA, et al. Robinin modulates TLR/NF-κB signaling pathway in oxidized LDL induced human peripheral blood mononuclear cells. Int Immunopharmacol. 2014 Jan;18(1):191-7. [Content Brief]

[2]. wenwen Zhang, et al. Robinin inhibits pancreatic cancer cell proliferation, EMT and inflammation via regulating TLR2-PI3k-AKT signaling pathway, 10 May 2023.

[3]. Tsiklauri L, et al. Bioflavonoid Robinin from Astragalus falcatus Lam. Mildly Improves the Effect of Metothrexate in Rats with Adjuvant Arthritis. Nutrients. 2021 Apr 13;13(4):1268. [Content Brief]

[4]. Janeesh PA, et al. Robinin modulates doxorubicin-induced cardiac apoptosis by TGF-β1 signaling pathway in Sprague Dawley rats. Biomed Pharmacother. 2014 Oct;68(8):989-98. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.3501 mL	6.7507 mL	13.5015 mL	33.7537 mL
	5 mM	0.2700 mL	1.3501 mL	2.7003 mL	6.7507 mL
	10 mM	0.1350 mL	0.6751 mL	1.3501 mL	3.3754 mL
	15 mM	0.0900 mL	0.4500 mL	0.9001 mL	2.2502 mL
	20 mM	0.0675 mL	0.3375 mL	0.6751 mL	1.6877 mL
	25 mM	0.0540 mL	0.2700 mL	0.5401 mL	1.3501 mL
	30 mM	0.0450 mL	0.2250 mL	0.4500 mL	1.1251 mL
	40 mM	0.0338 mL	0.1688 mL	0.3375 mL	0.8438 mL
	50 mM	0.0270 mL	0.1350 mL	0.2700 mL	0.6751 mL
	60 mM	0.0225 mL	0.1125 mL	0.2250 mL	0.5626 mL
	80 mM	0.0169 mL	0.0844 mL	0.1688 mL	0.4219 mL
	100 mM	0.0135 mL	0.0675 mL	0.1350 mL	0.3375 mL

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Robinin

Apoptosis Compound Library

Immunology/Inflammation Compound Library

NF-κB Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Small Molecule Immuno-Oncology Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Glycoside Compound Library

Oxygen Sensing Compound Library

Medicine Food Homology Compound Library

Pyroptosis Compound Library

Traditional Chinese Medicine Active Compound Library

Flavonoids Library

Anti-Obesity Compound Library

Food-Sourced Compound Library

Anti-Cancer Natural Product Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Heterocyclic Compound Library

Pain-Related Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Autoimmune Disease Compound Library

Cell Death Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Anti-Fibrosis Compound Library

Miao Ethnicity Medicine Compound Library

Tibetan Medicine Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Anti-Rheumatic Arthritis Compound Library

Mass Spectrometry Human Metabolite Library

Antitussive and Antiasthmatic Traditional Chinese Medicine Active Compound Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Pattern Recognition Receptors Library

Mongolian Medicine Compound Library

Natural Product Diversity Scaffold Library

Tonifying Traditional Chinese Medicine Monomer Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Robinin

All Toll-like Receptor (TLR) Isoforms

Biological Activity

Chemical Information

Publications (5)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Robinin Related Classifications

Cat. No.: HY-N1346