Verrucarin J  (Synonyms: Muconomycin B)

Verrucarin J (Muconomycin B) is a metabolite of the Myrothecium fungus family. Verrucarin J generates reactive oxygen species (ROS) and induces apoptosis of cancer cell lines, such as A549, HCT 116 and SW-620 cells. Verrucarin J shows activities against Candida albicans and Mucor miehei. Verrucarin J inhibits arenavirus Junin (JUNV) yield with an IC₅₀ of 1.2 ng/mL^{[1][2][3][4][5]}.

Verrucarin J (0, 5, 10, 20, 50 nM; 24 hours) induces the apoptosis of A549 cells^[1].
Verrucarin J (0, 1, 2, 5, 10, 20, 50 nM; 24, 48, 72 hours) significantly inhibits cell proliferation of A549 and H1793 cells with IC₅₀ values of approximately 10 nM and 20 nM after 48 h of treatment, respectively^[1].
Verrucarin J (0, 0.1, 0.2, 0.3, 0.4, 0.5 μM; 24 hours) has an IC₅₀ of 300 nM for HCT 116 and SW-620 cell proliferation^[2].
Verrucarin J (0, 10, 20 nM, 48 hours) inhibits cancer stem cell (CSC) self-renewal pathways Wnt1/β-catenin and Notch1 and down-regulates the expression of key CSC specific genes (ALDH1, LGR5, OCT4 and CD133) of A549 cells^[1].
Verrucarin J (compound 2; 50 μg/disk) shows noteworthy activities against Candida albicans and Mucor miehei^[3].
Verrucarin J reduces JUNV yield more than 2 log units and has a similar effect against the arenavirus Tacaribe^[4].
Verrucarin J reduces the cell viability of Vero cells with a cytotoxic concentration 50% (CC₅₀) of 8.2 ng/mL^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Verrucarin J (0.5 mg/kg; i.p. for 4 weeks) suppresses AKT-induced tumor growth in a xenograft model^[2].
Verrucarin J (0.1, 0.5, 2.0 mg/kg; i.p. for three weeks) is a highly potent anticancer drug and suppresses tumor growth and metastasis^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

484.54

C₂₇H₃₂O₈

4643-58-7

Solid

White to off-white

CC1=C[C@@H]2[C@]3(COC(/C=C(C)/CCOC(/C=C/C=C\C(OC4[C@]3(C)[C@@]5([C@H](O2)C4)CO5)=O)=O)=O)CC1

Room temperature in continental US; may vary elsewhere.

• [1]. Udoh K, et al. Targeting of Lung Cancer Stem Cell Self-Renewal Pathway by a Small Molecule Verrucarin J. Stem Cell Rev Rep. 2019 Aug;15(4):601-611.  [Content Brief]

  [2]. Pal D, et al. Suppression of Notch1 and AKT mediated epithelial to mesenchymal transition by Verrucarin J in metastatic colon cancer. Cell Death Dis. 2018 Jul 23;9(8):798.  [Content Brief]

  [3]. Mondol MA, et al. Macrocyclic Trichothecenes from Myrothecium roridum Strain M10 with Motility Inhibitory and Zoosporicidal Activities against Phytophthora nicotianae. J Agric Food Chem. 2015 Oct 14;63(40):8777-86.  [Content Brief]

  [4]. García CC, et al, Damonte EB. Evaluation of the antiviral activity against Junin virus of macrocyclic trichothecenes produced by the hypocrealean epibiont of Baccharis coridifolia. Planta Med. 2002 Mar;68(3):209-12.  [Content Brief]

  [5]. Carter K, et al. Verrucarin J inhibits ovarian cancer and targets cancer stem cells. Oncotarget. 2017 Oct 6;8(54):92743-92756.  [Content Brief]