VU0080241
Based on 1 Customer Validation
VU0080241 is a positive allosteric modulator (PAM) of the metabotropic glutamate receptor subtype 4 (mGluR4), with an EC50 of 4.6 μM.
For research use only. We do not sell to patients.
- Purity: 99.69%
- CAS No.: 393845-24-4
- Formula: C19H23N5
- Molecular Weight:321.42
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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GPCR/G Protein Compound Library
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Allosteric Modulators (AMs) Compound Library
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Biological Activity
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mGlu4 4.6 μM (EC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | EC50 |
5 μM
Compound: 7, VU0080241
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Activity at rat mGluR5 expressed in HEK cells assessed as potentiation of glutamate-induced calcium flux by fluorescence assay
Activity at rat mGluR5 expressed in HEK cells assessed as potentiation of glutamate-induced calcium flux by fluorescence assay
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[PMID: 18793851] |
Chemical Information
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CAS No. 393845-24-4
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Appearance Solid
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Molecular Weight 321.42
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Formula C19H23N5
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Color Yellow to orange
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SMILES
CC1CN(CCC1)C2=C3C(N(N=C3)C4=CC=C(C=C4C)C)=NC=N2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 50 mg/mL (155.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (7.78 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1112 mL | 15.5560 mL | 31.1119 mL | 77.7798 mL |
| 5 mM | 0.6222 mL | 3.1112 mL | 6.2224 mL | 15.5560 mL | |
| 10 mM | 0.3111 mL | 1.5556 mL | 3.1112 mL | 7.7780 mL | |
| 15 mM | 0.2074 mL | 1.0371 mL | 2.0741 mL | 5.1853 mL | |
| 20 mM | 0.1556 mL | 0.7778 mL | 1.5556 mL | 3.8890 mL | |
| 25 mM | 0.1244 mL | 0.6222 mL | 1.2445 mL | 3.1112 mL | |
| 30 mM | 0.1037 mL | 0.5185 mL | 1.0371 mL | 2.5927 mL | |
| 40 mM | 0.0778 mL | 0.3889 mL | 0.7778 mL | 1.9445 mL | |
| 50 mM | 0.0622 mL | 0.3111 mL | 0.6222 mL | 1.5556 mL | |
| 60 mM | 0.0519 mL | 0.2593 mL | 0.5185 mL | 1.2963 mL | |
| 80 mM | 0.0389 mL | 0.1944 mL | 0.3889 mL | 0.9722 mL | |
| 100 mM | 0.0311 mL | 0.1556 mL | 0.3111 mL | 0.7778 mL |
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