Cat. No.: HY-134819A

(1R,2R)-ML-SI3

(Synonyms: (-)-trans-ML-SI3)

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

(1R,2R)-ML-SI3 is an isomer of ML-SI3 and a potent inhibitor of three isoforms of TRPML. (1R,2R)-ML-SI3 inhibits TRPMLs with IC₅₀s of 1.6 μM (TRPML1), 2.3 μM (TRPML2), and 12.5 μM (TRPML3), respectively.

For research use only. We do not sell to patients.

Purity: 99.79%
CAS No.: 2418594-00-8
Formula: C₂₃H₃₁N₃O₃S
Molecular Weight:429.58

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

(1R,2R)-ML-SI3 Chemical Structure

CAS No.: 2418594-00-8

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

TRPML1

1.6 μM (IC₅₀)

TRPML2

2.3 μM (IC₅₀)

TRPML3

12.5 μM (IC₅₀)

All TRP Channel Isoforms :

In Vitro

Table: Activation and inhibition of the three subtypes of TRPML by the ML-SI3-type compounds^[1]

Product_Names	Catalog_No	EC₅₀ for activation [μM]			IC₅₀ for inhibition [μM]
Product_Names	Catalog_No	TRPML1	TRPML2	TRPML3	TRPML1	TRPML2	TRPML3
(rel)-ML-SI3	HY-139426A	-	3.3±1.5	-	3.1±1.5	-	28.5±15.9
(cis)-ML-SI3	/	-	9.4±2.7	29.0±4.1	18.5±1.9	-	-
(1S,2S)-ML-SI3	HY-134819	-	2.7±1.4	10.8±0.5	5.9±1.7	-	-
(1R,2R)-ML-SI3	HY-134819A	-	-	-	1.6±0.7	2.3±1.1	12.5±7.6
Mean values ± SD of at least three independent experiments are shown. -: no effect.
ML-SI3	HY-139426	The mixtrue of (rel)-ML-SI3 and (cis)-ML-SI3

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 2418594-00-8

Appearance Solid

Molecular Weight 429.58

Formula C₂₃H₃₁N₃O₃S

Color White to off-white

SMILES

COC1=CC=CC=C1N2CCN([C@@H]3CCCC[C@H]3NS(C4=CC=CC=C4)(=O)=O)CC2

Synonyms

(-)-trans-ML-SI3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (3)

All Bio/Physico-chemical AssayFlow CytometryELISA

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Journal Impact Factor

Most Recent

Cell Calcium

MONNA alleviates MPTP-induced Parkinson's disease in zebrafish by activating TFEB dependently on ER Calcium. [Abstract]

2026 Mar:134:103125. PMID: 41637953

(1R,2R)-ML-SI3 purchased from MedChemExpress. Usage Cited in: Cell Calcium. 2026 Mar:134:103125. [Abstract]

TRPML1 currents density in lysosomes with or without (1R,2R)-ML-SI3 (ML-SI3, 30 μM; 6 h).

bioRxiv

2025 June 02.

bioRxiv

2025 January 01.

(1R,2R)-ML-SI3 purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 January 01.

RAW264.7 macrophages were treated with (1R,2R)-ML-SI3 (ML-SI3, 30-100 μM) and stimulated with Bafilomycin A1 (100 nM) for 4 hrs. Lysosomal exocytosis was measured by quantifying the cell surface abundance of LAMP-1 by FACS. Mean +/- SEM, n=3.

(1R,2R)-ML-SI3 purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 January 01.

RAW264.7 macrophages were treated with (1R,2R)-ML-SI3 (ML-SI3, 30-100 μM) and stimulated with Bafilomycin A1 (10 nM) overnight. GPNMB secretion was measured by ELISA. Mean +/- SEM, n=3.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (232.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3279 mL

11.6393 mL

23.2786 mL

5 mM

0.4656 mL

2.3279 mL

4.6557 mL

10 mM

0.2328 mL

1.1639 mL

2.3279 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:

To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg

Animal Weight (Per Animal) Animal Weight (Per Animal)
g

Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

%
+

+
%
Tween-80 + +

%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact
MedChemExpress (MCE) .

Tel: 609-228-6898
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Method for preparing in vivo working solution for animal experiments:
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Saline .

Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)

SDS (393 KB)

English - EN (393 KB)

Français - FR (393 KB)

Deutsch - DE (393 KB)

Norwegian - NO (393 KB)

Español - ES (393 KB)

Swedish - SV (393 KB)

Italian - IT (393 KB)

Korean - KR (393 KB)

Portuguese - PT (393 KB)

COA (2659 KB)

Elemental Analysis Report (2659 KB)

HNMR (2659 KB)

CNMR (2659 KB)

RP-HPLC (2659 KB)

NP-HPLC (2659 KB)

MS (2659 KB)

LCMS (2659 KB)

IR (2659 KB)

GC (2659 KB)

OR (2659 KB)

SDS-PAGE (2659 KB)

SEC-HPLC (2659 KB)

Other Report (2659 KB)

Handling Instructions (2659 KB)

References

[1]. Leser C, Keller M, Gerndt S, et al. Chemical and pharmacological characterization of the TRPML calcium channel blockers ML-SI1 and ML-SI3. Eur J Med Chem. 2021;210:112966. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg

DMSO 1 mM 2.3279 mL 11.6393 mL 23.2786 mL 58.1964 mL

5 mM 0.4656 mL 2.3279 mL 4.6557 mL 11.6393 mL

10 mM 0.2328 mL 1.1639 mL 2.3279 mL 5.8196 mL

15 mM 0.1552 mL 0.7760 mL 1.5519 mL 3.8798 mL

20 mM 0.1164 mL 0.5820 mL 1.1639 mL 2.9098 mL

25 mM 0.0931 mL 0.4656 mL 0.9311 mL 2.3279 mL

30 mM 0.0776 mL 0.3880 mL 0.7760 mL 1.9399 mL

40 mM 0.0582 mL 0.2910 mL 0.5820 mL 1.4549 mL

50 mM 0.0466 mL 0.2328 mL 0.4656 mL 1.1639 mL

60 mM 0.0388 mL 0.1940 mL 0.3880 mL 0.9699 mL

80 mM 0.0291 mL 0.1455 mL 0.2910 mL 0.7275 mL

100 mM 0.0233 mL 0.1164 mL 0.2328 mL 0.5820 mL

(1R,2R)-ML-SI3 Related Classifications

HY-134819A Related Classifications

Membrane Transporter/Ion Channel Neuronal Signaling

TRP Channel

Disease Areas

Neurological, Eye or Ear Disease

Pain

Neurological Disease

Powered by Bioz See more details on Bioz

Keywords

(1R,2R)-ML-SI3
2418594-00-8
(-)-trans-ML-SI3
TRP Channel
TRPML calcium channel blocker
lysosomal
endosomal functions
mutations
Inhibitor
inhibitor
inhibit

Your Recently Viewed Products

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3279 mL	11.6393 mL	23.2786 mL	58.1964 mL
5 mM	0.4656 mL	2.3279 mL	4.6557 mL	11.6393 mL
10 mM	0.2328 mL	1.1639 mL	2.3279 mL	5.8196 mL
15 mM	0.1552 mL	0.7760 mL	1.5519 mL	3.8798 mL
20 mM	0.1164 mL	0.5820 mL	1.1639 mL	2.9098 mL
25 mM	0.0931 mL	0.4656 mL	0.9311 mL	2.3279 mL
30 mM	0.0776 mL	0.3880 mL	0.7760 mL	1.9399 mL
40 mM	0.0582 mL	0.2910 mL	0.5820 mL	1.4549 mL
50 mM	0.0466 mL	0.2328 mL	0.4656 mL	1.1639 mL
60 mM	0.0388 mL	0.1940 mL	0.3880 mL	0.9699 mL
80 mM	0.0291 mL	0.1455 mL	0.2910 mL	0.7275 mL
100 mM	0.0233 mL	0.1164 mL	0.2328 mL	0.5820 mL

(1R,2R)-ML-SI3

3 Cited Publications

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(1R,2R)-ML-SI3

Membrane Transporter/Ion Channel Compound Library

Neuronal Signaling Compound Library

Mechanoreceptors Compound Library

Pain-Related Compound Library

Membrane Protein-targeted Compound Library

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Publications Citing Use of MedChemExpress (MCE) (1R,2R)-ML-SI3

All TRP Channel Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table