Cat. No.: HY-134819

(1S,2S)-ML-SI3

(Synonyms: (+)-trans-ML-SI3)

고객리뷰

Based on 1 Customer Validation

(1S,2S)-ML-SI3 is a trans-isomer of ML-SI3 that targets all three isoforms of TRPML. (1S,2S)-ML-SI3 is an activator of TRPML2 and TRPML3 (EC₅₀=2.7 μM/10.8 μM) and a potent inhibitor of TRPML1 (IC₅₀=5.9 μM).

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity: 99.96%
CAS No.: 2563870-87-9
화학식: C₂₃H₃₁N₃O₃S
분자량:429.58

보관:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

(1S,2S)-ML-SI3 Chemical Structure

CAS No.: 2563870-87-9

사이즈

가격

재고

수량

Amount: USD 0.00

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

HY-139426A

(rel)-ML-SI3

Biological Activity
- 제품 설명
- IC₅₀ & Target
- In Vitro
Chemical Information
용액&용해도
- In Vitro
- In Vivo
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

IC₅₀ & Target

TRPML1

5.9 μM (IC₅₀)

TRPML2

2.7 μM (EC₅₀)

TRPML3

10.8 μM (EC₅₀)

All TRP Channel Isoforms :

In Vitro

Table: Activation and inhibition of the three subtypes of TRPML by the ML-SI3-type compounds^[1]

Product_Names	Catalog_No	EC₅₀ for activation [μM]			IC₅₀ for inhibition [μM]
Product_Names	Catalog_No	TRPML1	TRPML2	TRPML3	TRPML1	TRPML2	TRPML3
(rel)-ML-SI3	HY-139426A	-	3.3±1.5	-	3.1±1.5	-	28.5±15.9
(cis)-ML-SI3	/	-	9.4±2.7	29.0±4.1	18.5±1.9	-	-
(1S,2S)-ML-SI3	HY-134819	-	2.7±1.4	10.8±0.5	5.9±1.7	-	-
(1R,2R)-ML-SI3	HY-134819A	-	-	-	1.6±0.7	2.3±1.1	12.5±7.6
Mean values ± SD of at least three independent experiments are shown. -: no effect.
ML-SI3	HY-139426	The mixtrue of (rel)-ML-SI3 and (cis)-ML-SI3

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 2563870-87-9

Appearance Solid

분자량 429.58

화학식 C₂₃H₃₁N₃O₃S

Color White to off-white

SMILES

COC1=CC=CC=C1N2CCN([C@H]3CCCC[C@@H]3NS(C4=CC=CC=C4)(=O)=O)CC2

Synonyms

(+)-trans-ML-SI3

선적

Room temperature in continental US; may vary elsewhere.

보관

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

용액&용해도

In Vitro:

DMSO : 100 mg/mL (232.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3279 mL

11.6393 mL

23.2786 mL

5 mM

0.4656 mL

2.3279 mL

4.6557 mL

10 mM

0.2328 mL

1.1639 mL

2.3279 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

=
Concentration Concentration

×
Volume Volume

×
Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

×

Volume _(start) Volume _(start)

=

Concentration _(final) Concentration _(final)

×

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:

To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO    40% PEG300    5% Tween-80    45% Saline
Solubility: ≥ 2.5 mg/mL (5.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

Protocol 2

Add each solvent one by one: 10% DMSO    90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

Protocol 3

Add each solvent one by one: 10% DMSO    90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg

Animal Weight (Per Animal) Animal Weight (Per Animal)
g

Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL

Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

%
+

+
%
Tween-80 + +

%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact
MedChemExpress (MCE) .

Tel: 609-228-6898
E-mail: [email protected]
Technical Support: [email protected]

Method for preparing in vivo working solution for animal experiments:
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Saline .

Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)

SDS (252 KB)

English - EN (252 KB)

Français - FR (252 KB)

Deutsch - DE (252 KB)

Norwegian - NO (252 KB)

Español - ES (252 KB)

Swedish - SV (252 KB)

Italian - IT (252 KB)

Korean - KR (252 KB)

Portuguese - PT (252 KB)

COA (2659 KB)

Elemental Analysis Report (2659 KB)

HNMR (2659 KB)

CNMR (2659 KB)

RP-HPLC (2659 KB)

NP-HPLC (2659 KB)

MS (2659 KB)

LCMS (2659 KB)

IR (2659 KB)

GC (2659 KB)

OR (2659 KB)

SDS-PAGE (2659 KB)

SEC-HPLC (2659 KB)

Other Report (2659 KB)

Handling Instructions (2659 KB)

References

[1]. Leser C, et al. Chemical and pharmacological characterization of the TRPML calcium channel blockers ML-SI1 and ML-SI3. Eur J Med Chem. 2021 Jan 15;210:112966. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg

DMSO 1 mM 2.3279 mL 11.6393 mL 23.2786 mL 58.1964 mL

5 mM 0.4656 mL 2.3279 mL 4.6557 mL 11.6393 mL

10 mM 0.2328 mL 1.1639 mL 2.3279 mL 5.8196 mL

15 mM 0.1552 mL 0.7760 mL 1.5519 mL 3.8798 mL

20 mM 0.1164 mL 0.5820 mL 1.1639 mL 2.9098 mL

25 mM 0.0931 mL 0.4656 mL 0.9311 mL 2.3279 mL

30 mM 0.0776 mL 0.3880 mL 0.7760 mL 1.9399 mL

40 mM 0.0582 mL 0.2910 mL 0.5820 mL 1.4549 mL

50 mM 0.0466 mL 0.2328 mL 0.4656 mL 1.1639 mL

60 mM 0.0388 mL 0.1940 mL 0.3880 mL 0.9699 mL

80 mM 0.0291 mL 0.1455 mL 0.2910 mL 0.7275 mL

100 mM 0.0233 mL 0.1164 mL 0.2328 mL 0.5820 mL

(1S,2S)-ML-SI3 Related Classifications

HY-134819 Related Classifications

Membrane Transporter/Ion Channel Neuronal Signaling

TRP Channel

Disease Areas

Neurological, Eye or Ear Disease Cancer

Pain

Cancer

Cancer Targeted Therapy Cancer Metabolism and Metastasis

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Keywords

(1S,2S)-ML-SI3
2563870-87-9
(+)-trans-ML-SI3
TRP Channel
Inhibitor
inhibitor
inhibit

Your Recently Viewed Products

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3279 mL	11.6393 mL	23.2786 mL	58.1964 mL
5 mM	0.4656 mL	2.3279 mL	4.6557 mL	11.6393 mL
10 mM	0.2328 mL	1.1639 mL	2.3279 mL	5.8196 mL
15 mM	0.1552 mL	0.7760 mL	1.5519 mL	3.8798 mL
20 mM	0.1164 mL	0.5820 mL	1.1639 mL	2.9098 mL
25 mM	0.0931 mL	0.4656 mL	0.9311 mL	2.3279 mL
30 mM	0.0776 mL	0.3880 mL	0.7760 mL	1.9399 mL
40 mM	0.0582 mL	0.2910 mL	0.5820 mL	1.4549 mL
50 mM	0.0466 mL	0.2328 mL	0.4656 mL	1.1639 mL
60 mM	0.0388 mL	0.1940 mL	0.3880 mL	0.9699 mL
80 mM	0.0291 mL	0.1455 mL	0.2910 mL	0.7275 mL
100 mM	0.0233 mL	0.1164 mL	0.2328 mL	0.5820 mL

(1S,2S)-ML-SI3

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수량:

(1S,2S)-ML-SI3

(rel)-ML-SI3

All TRP Channel Isoforms

Biological Activity

Chemical Information

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

(1S,2S)-ML-SI3 Related Classifications

HY-134819 Related Classifications