5,7-Dihydroxy-4-methylcoumarin
Based on 1 Customer Validation
5,7-Dihydroxy-4-methylcoumarin is an antioxidant. 5,7-Dihydroxy-4-methylcoumarin protects mouse cochlear hair cells from Cisplatin-induced damage, enhances cell viability and inhibits apoptosis. 5,7-Dihydroxy-4-methylcoumarin downregulates phosphorylated JNK levels, increases the ratio of phosphorylated FoxO1 to total FoxO1, scavenges free radicals, reduces ROS accumulation, maintains mitochondrial membrane potential and alleviates mitochondrial dysfunction. 5,7-Dihydroxy-4-methylcoumarin downregulates the expression of caspase-3 and improves cell viability. 5,7-Dihydroxy-4-methylcoumarin can be used in studies related to ototoxicity.
For research use only. We do not sell to patients.
- Purity: 98.85%
- CAS No.: 2107-76-8
- Formula: C10H8O4
- Molecular Weight:192.17
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| U-937 | CC50 |
>2000 μM
Compound: 5
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Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay
Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay
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[PMID: 22925447] |
| U-937 | IC50 |
>2000 μM
Compound: 5
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Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting
Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting
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[PMID: 22925447] |
5,7-Dihydroxy-4-methylcoumarin (D4M) (50-150 μM; 2 h pretreatment followed by 24 h co-treatment with Cisplatin) dose-dependently increases the viability of HEI-OC1 cells exposed to Cisplatin (HY-17394), with the strongest protective effect observed at 100 μM[1].
5,7-Dihydroxy-4-methylcoumarin (100 μM; 2 h pretreatment followed by 24 h co-treatment with Cisplatin) protects hair cells in the apical, middle and basal turns of cochlear explants from postnatal day 3 C57BL/6 mice against Cisplatin-induced loss, reduces mitochondrial ROS accumulation in cells, and preserves spiral ganglion neurons in the middle turn[1].
5,7-Dihydroxy-4-methylcoumarin (100 μM; 2 h pretreatment followed by 24 h co-treatment with Cisplatin) inhibits Cisplatin-induced apoptosis of HEI-OC1 cells by downregulating the expression of caspase-3[1].
5,7-Dihydroxy-4-methylcoumarin (100 μM; 2 h pretreatment followed by 24 h co-treatment with cisplatin) downregulates the phosphorylation level of JNK, reduces the activation and nuclear localization of FoxO1 in HEI-OC1 cells exposed to cisplatin, inhibits mitochondrial ROS accumulation, and maintains mitochondrial membrane potential[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:House Ear Institute-Organ of Corti1 (HEI-OC1) cells
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Concentration:0, 50, 100, 150 μM
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Incubation Time:2 h pretreatment, then 24 h co-treatment with cisplatin
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Result:Increased HEI-OC1 cell viability in a dose-dependent manner compared to cells treated with Cisplatin alone.
Showed the highest cell viability at 100 μM, with viability significantly higher than the Cisplatin-only group.
Decreased viability slightly at 150 μM.
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Cell Line:HEI-OC1 cells
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Concentration:100 μM
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Incubation Time:2 h pretreatment, then 24 h co-treatment with cisplatin
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Result:Drastically reduced the apoptotic cell rate relative to the Cisplatin-only group.
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Cell Line:HEI-OC1 cells
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Concentration:100 μM
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Incubation Time:2 h pretreatment, then 24 h co-treatment with cisplatin
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Result:Lowered the cleaved caspase-3/caspase-3 protein expression ratio relative to the cisplatin-only group.\nReduced p-JNK/JNK and p-C-JUN/C-JUN protein ratios relative to the cisplatin-only group.
Lowered total FoxO1 expression relative to the cisplatin-only group.
Increased p-FoxO1/FoxO1 ratio relative to the cisplatin-only group.
Reduced nuclear FoxO1 localization relative to the cisplatin-only group.
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Cell Line:HEI-OC1 cells
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Concentration:100 μM
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Incubation Time:2 h pretreatment, then 24 h co-treatment with cisplatin
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Result:Reduced nuclear FoxO1 localization relative to the cisplatin-only group.
Chemical Information
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CAS No. 2107-76-8
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Appearance Solid
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Molecular Weight 192.17
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Formula C10H8O4
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Color Light yellow to yellow
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SMILES
O=C1C=C(C)C2=C(O)C=C(O)C=C2O1
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 25 mg/mL (130.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.01 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.01 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (289 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Li C, et al. 5,7-Dihydroxy-4-methylcoumarin modulates the JNK/FoxO1 signaling pathway to attenuate cisplatin-induced ototoxicity by suppressing oxidative stress and apoptosis in vitro. Biochim Biophys Acta Mol Cell Res. 2023;1870(4):119437. [Content Brief]
[2]. Céspedes CL, et al. Antifungal and antibacterial activities of Mexican tarragon (Tagetes lucida). J Agric Food Chem. 2006;54(10):3521-3527. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.2037 mL | 26.0186 mL | 52.0373 mL | 130.0931 mL |
| 5 mM | 1.0407 mL | 5.2037 mL | 10.4075 mL | 26.0186 mL | |
| 10 mM | 0.5204 mL | 2.6019 mL | 5.2037 mL | 13.0093 mL | |
| 15 mM | 0.3469 mL | 1.7346 mL | 3.4692 mL | 8.6729 mL | |
| 20 mM | 0.2602 mL | 1.3009 mL | 2.6019 mL | 6.5047 mL | |
| 25 mM | 0.2081 mL | 1.0407 mL | 2.0815 mL | 5.2037 mL | |
| 30 mM | 0.1735 mL | 0.8673 mL | 1.7346 mL | 4.3364 mL | |
| 40 mM | 0.1301 mL | 0.6505 mL | 1.3009 mL | 3.2523 mL | |
| 50 mM | 0.1041 mL | 0.5204 mL | 1.0407 mL | 2.6019 mL | |
| 60 mM | 0.0867 mL | 0.4336 mL | 0.8673 mL | 2.1682 mL | |
| 80 mM | 0.0650 mL | 0.3252 mL | 0.6505 mL | 1.6262 mL | |
| 100 mM | 0.0520 mL | 0.2602 mL | 0.5204 mL | 1.3009 mL |
5,7-Dihydroxy-4-methylcoumarin Related Classifications
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