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5-Ph-IAA 

Cat. No.: HY-134653
Handling Instructions

5-Ph-IAA is a derivative of IAA. 5-Ph-IAA, a ligand, establishes the auxin-inducible degron 2 (AID2) system together with an OsTIR1 (F74G) mutant. AID2 induces rapid and efficient depletion of mAID-fused proteins to study protein function in living cells, causing tumor suppression.

For research use only. We do not sell to patients.

5-Ph-IAA Chemical Structure

5-Ph-IAA Chemical Structure

CAS No. : 168649-23-8

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Description

5-Ph-IAA is a derivative of IAA. 5-Ph-IAA, a ligand, establishes the auxin-inducible degron 2 (AID2) system together with an OsTIR1 (F74G) mutant. AID2 induces rapid and efficient depletion of mAID-fused proteins to study protein function in living cells, causing tumor suppression[1].

In Vitro

HCT116 cells constitutively expressing OsTIR1(F74G) are exposed to 5-Ph-IAA (1 µM; 6 hours), leading to DHC1-mAC degradation, which means the AID2 system better than the original AID system in generating conditional mutant cell lines[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: HCT116 cells
Concentration: 1 µM
Incubation Time: 6 hours
Result: Depleted DHC1-mAC efficiently in HCT116 cells constitutively expressing OsTIR1(F74G).
In Vivo

5-Ph-IAA (0-10 mg/kg; intraperitoneally injection; every day; 7 days), used for the AID2 system ,is sufficient to deplete mAID-BRD4 and TOP2A-mAC, leading to tumour suppression[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Balb/c-nu female mice (7 weeks old; 16–20 g ) bearing mAID-BRD4 and TOP2A-mAC xenograft tumours cells[1]
Dosage: 0 mg/kg, 1 mg/kg, 3 mg/kg and 10 mg/kg
Administration: Intraperitoneally injection; every day; 7 days
Result: Displayed significant tumour suppression of mAID-BRD4 xenografts and TOP2A-mAC xenografts.
Molecular Weight

251.28

Formula

C₁₆H₁₃NO₂

CAS No.

168649-23-8

SMILES

O=C(O)CC1=CNC2=C1C=C(C3=CC=CC=C3)C=C2

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Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

5-Ph-IAAOthersAID2auxinOsTIR1antitumorInhibitorinhibitorinhibit

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5-Ph-IAA
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