Cat. No.: HY-N0787

Cryptochlorogenic acid

(Synonyms: 4-Caffeoylquinic acid; 4-O-Caffeoylquinic acid)

5 Cited Publications

Customer Review

Based on 5 publication(s) in Google Scholar

Cryptochlorogenic acid (4-Caffeoylquinic acid) is a naturally occurring phenolic acid compound with oral effectiveness, anti-inflammatory, antioxidant and anti-cardiac hypertrophy effects. Alleviating LPS (HY-D1056) and ISO (HY-B0468) by regulating proinflammatory factor expression, inhibiting NF-κB activity, promoting Nrf2 nuclear transfer, and regulating PI3Kα/Akt/ mTOR / HIF-1α signaling pathway Induced physiological stress response.

For research use only. We do not sell to patients.

Purity: 99.23%
CAS No.: 905-99-7
Formula: C₁₆H₁₈O₉
Molecular Weight:354.31

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Cryptochlorogenic acid Chemical Structure

CAS No.: 905-99-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (5)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

HIF-1α

All Endogenous Metabolite Isoforms :

All NF-κB Isoforms :

All mTOR Isoforms :

All HIF/HIF Prolyl-Hydroxylase Isoforms :

In Vitro

Cryptochlorogenic acid (0-150 μM, 12, 24 or 48 h) shows low toxicity to RAW264.7 cells and does not significantly affect the viability of RAW264.7 cells at specific concentrations^[2].
Cryptochlorogenic acid (20-80 μM, 2 h) can dose-dependent inhibit lipopolysaccharide (LPS: 1 μg/mL, 24 h) in RAW264.7 cells. induced the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), blocking the expression of iNOS, COX-2, TNF-α and IL-6^[2].
Cryptochlorogenic acid (20-80 μM, 2 hours) inhibits the phosphorylation of IκB kinase (IKK), degrades I-κB, and reduces the nuclear translocation of NF-κB. At the same time, CCGA downregulates the phosphorylation level of MAPKs. Overall, CCGA effectively controls the expression of pro-inflammatory factors, thereby alleviating LPS-induced (1 μg/mL, 24 h) inflammation. It also promotes the nuclear translocation of Nrf2 to inhibit oxidative stress^[2].
Cryptochlorogenic acid (1-200 μM, 48 hours) can effectively reduce the myocardial hypertrophy of H9c2 cells caused by ISO at a certain concentration. Cryptochlorogenic acid regulates the PI3Kα/Akt/mTOR/HIF-1α signaling pathway by significantly inhibiting the phosphorylation expression level of mTOR and over-expression of p-Akt and HIF-1α induced by ISO^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:

RAW264.7 cells
Concentration:

Cryptochlorogenic acid: 20-80 μM, LPS: 1 μg/mL
Incubation Time:

Cryptochlorogenic acid: 2 h, LPS: 24 h
Result:

Significantly inhibited the protein levels of iNOS, COX-2, IL-6, TNF-α and TLR4 in RAW264.7 macrophages stimulated by LPS.
Significantly inhibited the phosphorylation and degradation of IκB and the phosphorylation of IKKα/β induced by LPS.
Dose-dependent reduction of LPS-induced NF-κB(p65) transfer from cytoplasm to nucleus.
Significantly inhibited the phosphorylation of JNK1/2, ERK1/2, and p38 proteins induced by LPS.
significantly up-regulated Nrf2 protein levels in the nucleus and decreased NRF2 protein levels in the cytoplasm in a dose-dependent manner.

Cell Viability Assay^[2]

Cell Line:

RAW264.7 cells
Concentration:

Cryptochlorogenic acid (CCGA) : 0-150 μM, LPS (HY-D1056) : 0-3 μg/ml
Incubation Time:

12, 24 or 48 h
Result:

At doses of 150 μM and 100 μM, slight toxicity was shown to RAW264.7 cells with no significant decrease in cell viability.
RAW264.7 cells showed no toxicity after being treated with LPS at different concentrations for 12 hours. After being treated with LPS above 2 μg/ml for 24h, the cell viability decreased significantly.
Treated with 0-100 μM CCGA and 1 μg/ml LPS for 24 h, RAW264.7 cells had low toxicity and no significant effect on cell viability.

RT-PCR^[3]

Cell Line:

H9c2 Cells
Concentration:

1-200 μM
Incubation Time:

48 h
Result:

Significantly decreased the expression levels of ANP, BNP and HIF-1α mRNA in H9c2 cells after ISO treatment.

In Vivo

Pharmacokinetic parameters of Cryptochlorogenic acid after intragastric administration of Cryptochlorogenic acid at three dosages^[2]

Dose (mg/kg)	C_max (μg/L)	t_max (h)	t_1/2 (h)	AUC_0-t (μg•h/L)	AUC_0-∞ (μg•h/L)	MRT_0-t (h)	MRT_0-∞ (h)
100	630	0.33	2.00	1938.91	1977.70	3.21	3.51
200	1270.09	0.47	1.97	3071.87	3179.41	3.23	3.39
400	2582.68	0.44	2.34	8825.32	9139.54	3.47	3.93

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 905-99-7
Appearance Solid
Molecular Weight 354.31
Formula C₁₆H₁₈O₉
Color White to off-white
SMILES

O=C(/C=C/C1=CC(O)=C(O)C=C1)O[C@@H]([C@@H]2O)[C@@H](C[C@](C(O)=O)(O)C2)O
Synonyms

4-Caffeoylquinic acid; 4-O-Caffeoylquinic acid
Structure Classification
- Phenylpropanoids
- Simple Phenylpropanols
- Phenols
- Polyphenols
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (5)

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Journal Impact Factor

Phytomedicine

A novel mechanism of Reduning injection in sepsis treatment: Targeting inflammatory kinases TBK1 and IKKβ. [Abstract]

2026 Feb:151:157776. PMID: 41512388
Lwt-Food Sci Technol

December 2021, 112343.
Aquaculture

2026 Feb 12.
SSRN

2026 Apr 8.
Chemistry of Plant Raw Material

2025.

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (141.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8224 mL

14.1119 mL

28.2239 mL

5 mM

0.5645 mL

2.8224 mL

5.6448 mL

10 mM

0.2822 mL

1.4112 mL

2.8224 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (7.06 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (7.06 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.06 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Wang Jing, ea al.Simultaneous determination of chlorogenic acid,cryptochlorogenic acid,caffeic acid,naringin,hesperidin and linarin in Xiao′erjinning oral liquid by an HPLC method. China Journal of Chinese Materia Medica, 2010-13

[2]. Zhao XL, et al. Cryptochlorogenic acid attenuates LPS-induced inflammatory response and oxidative stress via upregulation of the Nrf2/HO-1 signaling pathway in RAW 264.7 macrophages. Int Immunopharmacol. 2020;83:106436. [Content Brief]

[3]. Li J, et al. Cryptochlorogenic acid and its metabolites ameliorate myocardial hypertrophy through a HIF1α-related pathway. Food Funct. 2022;13(4):2269-2282. Published 2022 Feb 21. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.8224 mL	14.1119 mL	28.2239 mL	70.5597 mL
	5 mM	0.5645 mL	2.8224 mL	5.6448 mL	14.1119 mL
	10 mM	0.2822 mL	1.4112 mL	2.8224 mL	7.0560 mL
	15 mM	0.1882 mL	0.9408 mL	1.8816 mL	4.7040 mL
	20 mM	0.1411 mL	0.7056 mL	1.4112 mL	3.5280 mL
	25 mM	0.1129 mL	0.5645 mL	1.1290 mL	2.8224 mL
	30 mM	0.0941 mL	0.4704 mL	0.9408 mL	2.3520 mL
	40 mM	0.0706 mL	0.3528 mL	0.7056 mL	1.7640 mL
	50 mM	0.0564 mL	0.2822 mL	0.5645 mL	1.4112 mL
	60 mM	0.0470 mL	0.2352 mL	0.4704 mL	1.1760 mL
	80 mM	0.0353 mL	0.1764 mL	0.3528 mL	0.8820 mL
	100 mM	0.0282 mL	0.1411 mL	0.2822 mL	0.7056 mL

Keywords

Cryptochlorogenic acid

5 Cited Publications

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Cryptochlorogenic acid

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Publications Citing Use of MedChemExpress (MCE) Cryptochlorogenic acid

All Endogenous Metabolite Isoforms

All NF-κB Isoforms

All mTOR Isoforms

All HIF/HIF Prolyl-Hydroxylase Isoforms

Biological Activity

Chemical Information

Publications (5)