Dihydrodaidzein
Based on 1 publication(s) in Google Scholar
Dihydrodaidzein is a dietary phytoestrogen. Dihydrodaidzein possesses functions including antioxidation, inhibition of DNA oxidative damage, regulation of lipid metabolism and maintenance of intestinal flora stability. Dihydrodaidzein induces apoptosis, regulates immune responses, modulates tumor-related factors, activates the Bmp-2 signaling pathway, inhibits NF-κB and MAPK, and exhibits anti-inflammatory activity. Dihydrodaidzein can be used in research related to cancer, cardiovascular diseases, osteoporosis and inflammatory diseases.
For research use only. We do not sell to patients.
- Purity: 99.38%
- CAS No.: 17238-05-0
- Formula: C15H12O4
- Molecular Weight:256.26
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Dihydrodaidzein
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
Dihydrodaidzein, which is obtained as a racemic mixture through the conversion of Daidzein (HY-N0019) by cell extracts of Slackia isoflavoniconvertens, can be further converted into Equol ((-)-(S)-Equol) (HY-100583)[1].
Dihydrodaidzein can be absorbed and converted into Equol ((-)-(S)-Equol) (HY-100583) by bacteria of the genus Lactococcus[3].
Dihydrodaidzein exhibits high effective permeability in artificial membrane and Caco-2 monolayer assays, indicating its superior intestinal permeability[5].
Dihydrodaidzein promotes osteogenesis in MC3T3-E1 cells via the Bmp2 pathway and inhibits osteoclastogenesis in RAW 264.7 cells by suppressing the NF-κB and MAPK pathways[5].
Dihydrodaidzein possesses favorable water solubility (0.14 g/L), exhibits definite solubility in various organic solvents and alkaline solutions, and has a pKa value of 7.71[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 17238-05-0
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Appearance Solid
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Molecular Weight 256.26
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Formula C15H12O4
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Color Off-white to gray
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SMILES
O=C1C(C2=CC=C(O)C=C2)COC3=CC(O)=CC=C13
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Synonyms
(±)-Dihydrodaidzein
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
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Journal Impact Factor
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Most Recent
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Biol Reprod
Associations between isoflavone exposure and reproductive damage in adult males: evidence from human and model system studies. [Abstract]2022 Nov 14;107(5):1360-1373. PMID: 35948002
Solvent & Solubility
DMSO : 100 mg/mL (390.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Schröder C, et al. Identification and expression of genes involved in the conversion of daidzein and genistein by the equol-forming bacterium Slackia isoflavoniconvertens. Applied and environmental microbiology. 2013 Jun;79(11):3494-502. [Content Brief]
[2]. Franke AA, et al. Liquid chromatographic-photodiode array mass spectrometric analysis of dietary phytoestrogens from human urine and blood. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 2002 Sep 25;777(1-2):45-59. [Content Brief]
[3]. Ubaid M, et al. Daidzein from Dietary Supplement to a Drug Candidate: An Evaluation of Potential. ACS omega. 2023 Sep 12;8(36):32271-32293. [Content Brief]
[4]. Shimada Y, et al. Cloning and expression of a novel NADP(H)-dependent daidzein reductase, an enzyme involved in the metabolism of daidzein, from equol-producing Lactococcus strain 20-92. Applied and environmental microbiology. 2010 Sep;76(17):5892-901. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.9023 mL | 19.5114 mL | 39.0229 mL | 97.5572 mL |
| 5 mM | 0.7805 mL | 3.9023 mL | 7.8046 mL | 19.5114 mL | |
| 10 mM | 0.3902 mL | 1.9511 mL | 3.9023 mL | 9.7557 mL | |
| 15 mM | 0.2602 mL | 1.3008 mL | 2.6015 mL | 6.5038 mL | |
| 20 mM | 0.1951 mL | 0.9756 mL | 1.9511 mL | 4.8779 mL | |
| 25 mM | 0.1561 mL | 0.7805 mL | 1.5609 mL | 3.9023 mL | |
| 30 mM | 0.1301 mL | 0.6504 mL | 1.3008 mL | 3.2519 mL | |
| 40 mM | 0.0976 mL | 0.4878 mL | 0.9756 mL | 2.4389 mL | |
| 50 mM | 0.0780 mL | 0.3902 mL | 0.7805 mL | 1.9511 mL | |
| 60 mM | 0.0650 mL | 0.3252 mL | 0.6504 mL | 1.6260 mL | |
| 80 mM | 0.0488 mL | 0.2439 mL | 0.4878 mL | 1.2195 mL | |
| 100 mM | 0.0390 mL | 0.1951 mL | 0.3902 mL | 0.9756 mL |
Dihydrodaidzein Related Classifications
HY-N1461 Related Classifications
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