Tyrphostin A1
Based on 1 publication(s) in Google Scholar
Tyrphostin A1 (Tyrphostin 1; AG9) is a tyrosine kinase inhibitor and an inactive control molecule for Tyrphostin A25 (HY-101958), with weak inhibitory activity against EGFR. Tyrphostin A1 inhibits the CD40 signaling pathway in macrophages, blocks NF‑κB nuclear translocation, downregulates IL‑12 p40 expression, suppresses Th1 cell differentiation, and alleviates experimental autoimmune encephalomyelitis in mice. Tyrphostin A1 can be used in research related to autoimmune inflammation.
For research use only. We do not sell to patients.
- Purity : 98.96%
- CAS No.: 2826-26-8
- Formula: C11H8N2O
- Molecular Weight:184.19
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
-
HY-19969
YM-90709IL-5 Receptor Antagonist -
HY-16668R
Tyrphostin A1 (Standard)Reference Standard -
HY-14644
ApilimodIL-12/IL-23 Inhibitor -
HY-15898
Y-320Apoptosis Inducer -
HY-14644A
Apilimod mesylateIL-12/IL-23 Inhibitor -
HY-15614
SC144GP130 Inhibitor -
HY-108841
RaleukinIL-1R Antagonist -
HY-B0122
TopiramateAntiepileptic Agent -
HY-N0038
AlantolactoneApoptosis Inducer -
HY-17002
Suplatast (Tosilate)Th2 Cytokine Inhibitor -
HY-N0283
DiacereinIL-1 Inhibitor -
HY-101757
NO-prednisolonePrednisolone Derivative
Publications Citing Use of MedChemExpress (MCE) Tyrphostin A1
MoreAll EGFR Isoforms
More
Biological Activity
Description
IC50 & Target
[1]|
IL-12 |
EGFR |
NF-κB |
In Vitro
Tyrphostin A1 blocks CD40L-induced NF-κB nuclear translocation and inhibits IL-12 p40 gene activation in mouse peritoneal macrophages[1].
Tyrphostin A1 (0.1-10 μM) inhibits MBP peptide-stimulated cell proliferation in HS‑17 Th1 cloned cells with an inhibition rate of 94%, but has no inhibitory effect on IL‑2- or IL‑12-mediated T cell proliferation[1].
Tyrphostin A1 is an EGFR inhibitor, and its combination with Hydroxyurea (HY-B0313) increases rAAV transduction efficiency by 12-fold in Hela cells without obvious cytotoxicity at the experimental concentration[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:mouse splenic macrophages
-
Concentration:0., 1, 10 μM
-
Incubation Time:30 min
-
Result:Inhibited CD40L-stimulated IL‑12 p40 secretion.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 2826-26-8
-
Appearance Solid
-
Molecular Weight 184.19
-
Formula C11H8N2O
-
Color Off-white to yellow
-
SMILES
N#C/C(C#N)=C\C1=CC=C(OC)C=C1
-
Synonyms
Tyrphostin 1; AG9
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
Pflugers Arch
Unlocking the potential: unveiling tyrphostins with Michael-reactive cyanoacrylate motif as promising inhibitors of human 5-lipoxygenase. [Abstract]2024 Dec;476(12):1913-1928. PMID: 39347835
Solvent & Solubility
In Vitro:
DMSO : ≥ 100 mg/mL (542.92 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (289 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
Hot Topics
-
All
-
Topics
-
Most Recent
-
Oldest
-
Topics The Tumor Microenvironment: A Pro-Tumor Niche and Therapeutic Target in Cancer
Cancer is not a single mass of cancer cells, but a complex ecosystem, the tumor microenvironment (TME).
2025-10-23
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.4292 mL | 27.1459 mL | 54.2918 mL | 135.7294 mL |
| 5 mM | 1.0858 mL | 5.4292 mL | 10.8584 mL | 27.1459 mL | |
| 10 mM | 0.5429 mL | 2.7146 mL | 5.4292 mL | 13.5729 mL | |
| 15 mM | 0.3619 mL | 1.8097 mL | 3.6195 mL | 9.0486 mL | |
| 20 mM | 0.2715 mL | 1.3573 mL | 2.7146 mL | 6.7865 mL | |
| 25 mM | 0.2172 mL | 1.0858 mL | 2.1717 mL | 5.4292 mL | |
| 30 mM | 0.1810 mL | 0.9049 mL | 1.8097 mL | 4.5243 mL | |
| 40 mM | 0.1357 mL | 0.6786 mL | 1.3573 mL | 3.3932 mL | |
| 50 mM | 0.1086 mL | 0.5429 mL | 1.0858 mL | 2.7146 mL | |
| 60 mM | 0.0905 mL | 0.4524 mL | 0.9049 mL | 2.2622 mL | |
| 80 mM | 0.0679 mL | 0.3393 mL | 0.6786 mL | 1.6966 mL | |
| 100 mM | 0.0543 mL | 0.2715 mL | 0.5429 mL | 1.3573 mL |
Tyrphostin A1 Related Classifications
HY-16668 Related Classifications
Powered by Bioz
Keywords
- Tyrphostin A1
- 2826-26-8
- Tyrphostin 1
- AG9
- Tyrphostin A 1
- Tyrphostin A-1
- Tyrphostin1
- Tyrphostin-1
- AG9
- AG 9
- AG-9
- Interleukin Related
- NF-κB
- EGFR
- IL
- Nuclear factor-κB
- Nuclear factor-kappaB
- Epidermal growth factor receptor
- ErbB-1
- HER1
- STAT3
- TNFalpha
- asthma
- cyclooxygenase
- endotoxic shock
- eosinophilic lung infiltration
- iNOS
- multiple organ dysfunction
- protein tyrosine kinases
- Inhibitor
- inhibitor
- inhibit