Anonaine
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Anonaine is an alkaloid. Anonaine exhibits high affinity for Bcl-2. Anonaine inhibits aromatic L-Amino acid decarboxylase, binds c-Jun, and exerts DNA damage, oxidative stress, cell cycle arrest, lipid peroxidation inhibition, antioxidant, cAMP-lowering, dopamine uptake inhibition, Antibacterial, and Antifungal activities. Anonaine does not reduce basal intracellular calcium and weakly reduces TH mRNA. Anonaine protects neurons by reducing Apoptosis and excitotoxicity. Anonaine can be used for research on cancer and multiple sclerosis.
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- Reinheit : 98.0%
- CAS. Nr.: 1862-41-5
- Formel: C17H15NO2
- Molecular Weight:265.31
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Speicherung:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biologische Aktivität
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Chemical Information
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Biologische Aktivität
Beschreibung
IC50 & Target
[1]|
Bcl-2 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Hepatocyte | IC50 |
70.3 μg/mL
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Cytotoxicity against primary cultured rat hepatocytes assessed as inhibition of MTT reduction after 24 hrs exposure by MTT assay.
Cytotoxicity against primary cultured rat hepatocytes assessed as inhibition of MTT reduction after 24 hrs exposure by MTT assay.
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18295494 |
| HepG2 | IC50 |
33.5 μg/mL
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Cytotoxicity against human HepG2 cells assessed as inhibition of MTT reduction after 24 hrs exposure by MTT assay.
Cytotoxicity against human HepG2 cells assessed as inhibition of MTT reduction after 24 hrs exposure by MTT assay.
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18295494 |
| HeLa | IC50 |
24.8 μg/mL
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Cytotoxicity against human HeLa cells assessed as inhibition of MTT reduction after 24 hrs exposure by MTT assay.
Cytotoxicity against human HeLa cells assessed as inhibition of MTT reduction after 24 hrs exposure by MTT assay.
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18295494 |
| PC-12 | IC50 |
0.05 μM
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Inhibition of intracellular dopamine content in rat PC12 pheochromocytoma cells measured by HPLC after derivatization following 24 hrs exposure.
Inhibition of intracellular dopamine content in rat PC12 pheochromocytoma cells measured by HPLC after derivatization following 24 hrs exposure.
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18305432 |
In Vitro
Anonaine (24 h) is cytotoxic to HeLa cells, with an IC50 of 24.8 µg/mL[3].
Anonaine (0.05 µM; 24-48 h) does not aggravate L-DOPA-induced cytotoxicity in PC12 cells, and slightly increases cell viability when used in combination[5].
Anonaine (0.01-0.2 µM; 24 h) decreases dopamine content in PC12 cells in a concentration-dependent manner, with an IC50 of 0.05 µM[5].
Anonaine (0.05 µM; 1-72 h) inhibits TH more strongly and for a longer duration than it inhibits AADC in PC12 cells[5].
Anonaine (0.05 µM; 0-48 h) only slightly reduces TH mRNA levels in PC12 cells[5].
Anonaine (0.05 µM; 10-60 min) decreases intracellular cyclic adenosine monophosphate levels in PC12 cells[5].
Anonaine (0.05 µM; 24 h) attenuates L-DOPA-induced increase in intracellular dopamine content in PC12 cells[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:PC12 rat pheochromocytoma cells
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Concentration:0.05 µM
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Incubation Time:10 and 60 min
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Result:Control cyclic AMP level was 241.6 pmol/mg protein.
Intracellular cyclic AMP levels were decreased to 40.1-41.3% of control at 10 and 60 min.
- Research Protocol for Infectious Diseases
- RNA extraction experimental
- Annexin V plus membrane-impermeant dye apoptosis staining
- ROS/oxidative-stress fluorescent staining
- BrdU Incorporation Assay
- Protocol for Cell Cycle
- Ca2+ Staining Technique
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Flow cytometric DNA-content cell-cycle staining
- Calcium Spark Assay
- Apoptosis Solutions
- Ferroptosis Solutions
- Genotoxicity/Mutagenicity Study
Chemical Information
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CAS. Nr. 1862-41-5
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Appearance Solid
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Molecular Weight 265.31
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Formel C17H15NO2
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Color Brown to black
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SMILES
[H][C@@]1(NCC2)CC3=CC=CC=C3C4=C1C2=CC5=C4OCO5
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Structure Classification
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Initial Source
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Reinheit & Dokumentation
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Data Sheet (283 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Anonaine Related Classifications
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