2. Cell Cycle/DNA Damage Metabolic Enzyme/Protease
  3. HSP
  4. Arimoclomol citrate

Arimoclomol citrate  (Synonyms: BRX-220 citrate)

Cat. No.: HY-106443B Purity: 99.76%
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Arimoclomol citrate (BRX-220 citrate) is an orally active co-inducer of heat shock proteins (HSP). Arimoclomol citrate protects motor neurons by enhancing Hsp expression, thus directly affecting protein aggregation and clearance of misfolded assemblies via the proteasome-ubiquitin system. Arimoclomol citrate can be used for the study of Niemann–Pick disease type C.

For research use only. We do not sell to patients.

CAS No. : 368860-21-3

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of Arimoclomol citrate:

Arimoclomol citrate Related Antibodies

Top Publications Citing Use of Products

    Arimoclomol citrate purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 May 16;25(10):5417.  [Abstract]

    Representative western blotting showing the effect of Arimoclomol maleate (Ari: 300 μM) treatments on protein expression in PS1 (PS1KO) and PS2 KO (PS2KO) mouse embryonic fibroblasts (MEFs).

    Arimoclomol citrate purchased from MedChemExpress. Usage Cited in: Exp Eye Res. 2019 Nov;188:107792.  [Abstract]

    Western blot was used to detect the expression of HSP70 in ARPE-19 cells stimulated by Arimoclomol maleate (10 μM) for 24 h.

    Arimoclomol citrate purchased from MedChemExpress. Usage Cited in: Exp Eye Res. 2019 Nov;188:107792.  [Abstract]

    ARPE-19 cells were simultaneously treated with 4-HNE and Arimoclomol (10 μM) for 24 h, then IL-1β levels in supernatant of ARPE-19 cells were detected by corresponding ELISA kit.

    View All HSP Isoform Specific Products:

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    HSP40 HSP70 HSP90 HSP105 HSF1 HSP HSPA5

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Arimoclomol citrate (BRX-220 citrate) is an orally active co-inducer of heat shock proteins (HSP)[1]. Arimoclomol citrate protects motor neurons by enhancing Hsp expression, thus directly affecting protein aggregation and clearance of misfolded assemblies via the proteasome-ubiquitin system[2]. Arimoclomol citrate can be used for the study of Niemann–Pick disease type C.

    IC50 & Target[1]

    HSP

     

    In Vivo

    Arimoclomol (BRX-220; 20 mg/kg; orally administered for 5 d) has a protective effect against cholecystokinin-octapeptide (CCK)-induced acute pancreatitis[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Wistar rats weighing 240 to 270 g[1]
    Dosage: 20 mg/kg
    Administration: Administered intragastrically, for 5 days
    Result: Had a protective effect against CCK-induced acute pancreatitis.
    Molecular Weight

    505.90

    Formula

    C20H28ClN3O10
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    Cl/C(C1=C[N+]([O-])=CC=C1)=N\OC[C@H](O)CN2CCCCC2.O=C(CC(C(O)=O)(O)CC(O)=O)O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 35 mg/mL (69.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9767 mL 9.8834 mL 19.7668 mL
    5 mM 0.3953 mL 1.9767 mL 3.9534 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 5 mg/mL (9.88 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 5 mg/mL (9.88 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.76%

    SDS (557 KB)

    COA (253 KB) HNMR (270 KB) LCMS (94 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.9767 mL 9.8834 mL 19.7668 mL 49.4169 mL
    5 mM 0.3953 mL 1.9767 mL 3.9534 mL 9.8834 mL
    10 mM 0.1977 mL 0.9883 mL 1.9767 mL 4.9417 mL
    15 mM 0.1318 mL 0.6589 mL 1.3178 mL 3.2945 mL
    20 mM 0.0988 mL 0.4942 mL 0.9883 mL 2.4708 mL
    25 mM 0.0791 mL 0.3953 mL 0.7907 mL 1.9767 mL
    30 mM 0.0659 mL 0.3294 mL 0.6589 mL 1.6472 mL
    40 mM 0.0494 mL 0.2471 mL 0.4942 mL 1.2354 mL
    50 mM 0.0395 mL 0.1977 mL 0.3953 mL 0.9883 mL
    60 mM 0.0329 mL 0.1647 mL 0.3294 mL 0.8236 mL
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    Arimoclomol citrate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Arimoclomol368860-21-3BRX-220BRX220BRX 220HSPHeat shock proteinsco-inducermotorneuronsproteinaggregationclearancemisfoldedassembliesproteasomeubiquitinsystemInhibitorinhibitorinhibit

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