Art. -Nr.: HY-B0317

Amlodipine

15 Cited Publications

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Based on 15 publication(s) in Google Scholar

Amlodipine, an antianginal agent and an orally active dihydropyridine calcium channel blocker, works by blocking the voltage-dependent L-type calcium channels, thereby inhibiting the initial influx of calcium. Amlodipine can be used for the research of high blood pressure and cancer.

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Reinheit: 99.87%
CAS. Nr.: 88150-42-9
Formel: C₂₀H₂₅ClN₂O₅
Molecular Weight:408.88

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

Amlodipine Chemical Structure

CAS. Nr.: 88150-42-9

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (15)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target^[1]

L-type calcium channel

Alle Calcium Channel Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
LLC-MK2	IC₅₀	70.82 μM Compound: Amlodipine	Cytotoxicity against rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay Cytotoxicity against rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay	[PMID: 20934347]
LLC-MK2	IC₅₀	8.07 μM Compound: Amlodipine	Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi infected in rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi infected in rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay	[PMID: 20934347]
LLC-PK1	IC₅₀	22 μM Compound: Amlodipine	TP_TRANSPORTER: inhibition of Daunorubicin transepithelial transport (basal to apical) (Daunorubicin: 0.035 uM) in MDR1-expressing LLC-PK1 cells TP_TRANSPORTER: inhibition of Daunorubicin transepithelial transport (basal to apical) (Daunorubicin: 0.035 uM) in MDR1-expressing LLC-PK1 cells	[PMID: 11145223]
Ventricular myocyte	IC₅₀	0.57 μM Compound: Amlodipine	Inhibition of L-type calcium channel measured using whole-cell patch clamp in guinea pig ventricular myocytes Inhibition of L-type calcium channel measured using whole-cell patch clamp in guinea pig ventricular myocytes	[PMID: 22761000]

In Vitro

Amlodipine (20-40 μM; 48 h) reduces BrdU incorporation to 68.6% and 26.3% at concentrations of 20 and 30 μM in A431 cells, respectively^[3].
Amlodipine (30 μM; pretreated for 1 h) significantly attenuates the uridine 5′-triphosphate (UTP)-induced increases of [Ca²⁺]_i in A431 cells^[3].
Amlodipine (30 μM) inhibits the store-operated Ca²⁺influx evoked by Thapsigargin in Fluo-3-loaded cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Amlodipine (5 mg/kg/day; s.c. for 2 weeks) significantly decreases systolic blood pressure (SBP) in VSMC ATP2B1 KO mice^[4].
Amlodipine (10 mg/kg; i.p. once daily for 20 days) causes a significant retardation of tumor growth and prolongs the survival of A431 tumor-bearing mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

ATP2B1^loxP/loxP mice^[4]
Dosage:

5 mg/kg/day
Administration:

Subcutaneously implanted osmotic pump for 2 weeks
Result:

Significantly decreased the blood pressure.

Chemical Information

CAS. Nr. 88150-42-9
Appearance Solid
Molecular Weight 408.88
Formel C₂₀H₂₅ClN₂O₅
Color White to off-white
SMILES

O=C(OCC)C1=C(NC(C)=C(C1C2=CC=CC=C2Cl)C(OC)=O)COCCN
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (15)

All Cell Imaging/StainingCell Proliferation/Viability AssayFlow CytometryIn Vivo Efficacy StudyHistological Imaging/Staining

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Journal Impact Factor

J Adv Res

Calcium Influx: An Essential Process by which α-Synuclein Regulates Morphology of Erythrocytes. [Abstract]

2024 Aug:62:187-198. PMID: 37714326
Exp Mol Med

Sublytic C5b-9 induces glomerular mesangial cell proliferation via ERK1/2-dependent SOX9 phosphorylation and acetylation by enhancing Cyclin D1 in rat Thy-1 nephritis. [Abstract]

2021 Apr;53(4):572-590. PMID: 33811247

Amlodipine purchased from MedChemExpress. Usage Cited in: Exp Mol Med. 2021 Apr;53(4):572-590. [Abstract]

Rat GMCs pretreated with the calcium channel blocker Amlodipine (10μM), inhibitors of PKC (Go6983; 10μM), c-Raf (AZ628, 10μM) and MEK1/2 (U0126, 10μM) for 30min were stimulated with sublytic C5b-9 for 4 0min or 2h. The intracellular calcium was determined by Fluo-4 AM. The results showed that Amlodipine effectively blocked the rise in intracellular calcium levels in rat GMCs induced by 40 min of sublytic C5b-9 stimulation.
Sci Adv

WWC proteins-mediated compensatory mechanism restricts schwannomatosis driven by NF2 loss of function. [Abstract]

2025 Jan 24;11(4):eadp4765. PMID: 39841844
Br J Pharmacol

Amlodipine, an anti-hypertensive drug, alleviates non-alcoholic fatty liver disease by modulating gut microbiota. [Abstract]

2022 May;179(9):2054-2077. PMID: 34862599
Cell Rep

Calcium influx drives m6A-dependent RUNX1T1 splicing to promote adipogenic commitment. [Abstract]

2026 May 28;45(6):117471. PMID: 42213774
Acta Physiol

Tacrolimus Induced Hypertension and Vascular Remodeling Includes Mechanisms of Cellular Senescence-The Protective Effect of Valsartan. [Abstract]

2026 Apr;242(4):e70189. PMID: 41814130
Cells

Calcium-Related Genes Predicting Outcomes and Serving as Therapeutic Targets in Endometrial Cancer. [Abstract]

2022 Oct 8;11(19):3156. PMID: 36231119
Food Chem Toxicol

Coexposure to fluoride and sulfur dioxide aggravates enamel mineralization disorders in mice by disrupting calcium homeostasis-mediated endoplasmic reticulum stress. [Abstract]

2025 Feb 10:115317. PMID: 39938609

Amlodipine purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2025 Feb 10:115317. [Abstract]

A CCK-8 assay was used to detect the effect of AMs on the proliferation of Ishikawa cells. The results showed that the half-maximal inhibitory concentration (IC₅₀) of Amlodipine (AM) against Ishikawa cells (at 72 h) was 12.36 μM.

Amlodipine purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2025 Feb 10:115317. [Abstract]

Cell apoptosis rate was detected by flow cytometry using Annexin-V/PI staining. The results showed that the apoptotic rate of the Amlodipine (AM) (15 μM; 12 h)-treated Ishikawa cells was 16.8%, which was 2.16-fold higher than that in cells treated with DMSO.

Amlodipine purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2025 Feb 10:115317. [Abstract]

EdU staining showing the effect of Amlodipine (AM) (15 μM; 48 h) on Ishikawa cells. The EdU staining assay revealed that the EdU-positive rate of the AM-treated Ishikawa cells was significantly reduced compared with that of the control group.

Amlodipine purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2025 Feb 10:115317. [Abstract]

Amlodipine (AM) (15 or 20 mg/kg; i.p.; every other day for two weeks) inhibited tumor growth in a dose-dependent manner in BALB/c nude mice bearing subcutaneous xenografts established from Ishikawa cells.

Amlodipine purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2025 Feb 10:115317. [Abstract]

H&E and PCNA staining of sections from mice treated with Amlodipine (AM) (15 or 20 mg/kg; i.p.; every other day for two weeks).
J Biochem Mol Toxicol

An in vivo and in vitro model on the protective effect of cilnidipine on contrast-induced nephropathy via regulation of apoptosis and CaMKⅡ/mPTP pathway. [Abstract]

2022 Oct 7;e23238. PMID: 36207783
J Chem Thermodyn

2021, 106495.
Biochem Biophys Res Commun

Combinatorial screening of a panel of FDA-approved drugs identifies several candidates with anti-Ebola activities. [Abstract]

2020 Feb 19;522(4):862-868. PMID: 31806372
Nat Prod Commun

2024 Mar 20.
Dissolut Technol

2021 Jun.
SSRN

2025 Nov 6.
medRxiv

2025 May 21.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 31.25 mg/mL (76.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4457 mL

12.2285 mL

24.4571 mL

5 mM

0.4891 mL

2.4457 mL

4.8914 mL

10 mM

0.2446 mL

1.2229 mL

2.4457 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 3 mg/mL (7.34 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 3 mg/mL (7.34 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 3 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 3 mg/mL (7.34 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (889 KB)
- English - EN (889 KB)
- Français - FR (889 KB)
- Deutsch - DE (889 KB)
- Norwegian - NO (889 KB)
- Español - ES (889 KB)
- Swedish - SV (889 KB)
- Italian - IT (889 KB)
- Korean - KR (889 KB)
- Portuguese - PT (889 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Kishen G. Bulsara, et al. Amlodipine.

[2]. Haria M, et al. Amlodipine. A reappraisal of its pharmacological properties and therapeutic use in cardiovascular disease [published correction appears in Drugs 1995 Nov;50(5):896]. Drugs. 1995;50(3):560-586. [Content Brief]

[3]. Yoshida J, et, al. Antitumor effects of amlodipine, a Ca2+ channel blocker, on human epidermoid carcinoma A431 cells in vitro and in vivo. Eur J Pharmacol. 2004 May 25;492(2-3):103-12. [Content Brief]

[4]. Okuyama Y, et, al. The effects of anti-hypertensive drugs and the mechanism of hypertension in vascular smooth muscle cell-specific ATP2B1 knockout mice. Hypertens Res. 2018 Feb;41(2):80-87. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4457 mL	12.2285 mL	24.4571 mL	61.1426 mL
	5 mM	0.4891 mL	2.4457 mL	4.8914 mL	12.2285 mL
	10 mM	0.2446 mL	1.2229 mL	2.4457 mL	6.1143 mL
	15 mM	0.1630 mL	0.8152 mL	1.6305 mL	4.0762 mL
	20 mM	0.1223 mL	0.6114 mL	1.2229 mL	3.0571 mL
	25 mM	0.0978 mL	0.4891 mL	0.9783 mL	2.4457 mL
	30 mM	0.0815 mL	0.4076 mL	0.8152 mL	2.0381 mL
	40 mM	0.0611 mL	0.3057 mL	0.6114 mL	1.5286 mL
	50 mM	0.0489 mL	0.2446 mL	0.4891 mL	1.2229 mL
	60 mM	0.0408 mL	0.2038 mL	0.4076 mL	1.0190 mL

Keywords

Amlodipine

15 Cited Publications

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Amlodipine

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Publications Citing Use of MedChemExpress (MCE) Amlodipine

Alle Calcium Channel Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (15)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Amlodipine Related Classifications

HY-B0317 Related Classifications