Art. -Nr.: HY-13065

Isobavachalcone

(Synonyms: Corylifolinin; Isobacachalcone)

10 Cited Publications

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Based on 10 publication(s) in Google Scholar

Isobavachalcone (Corylifolinin) is derived from Psoralea corylifolia Linn. and is a potent inhibitor of Akt signaling pathway, which induces apoptosis in human cancer cells (Inhibits OVCAR-8 cell growth with an IC₅₀ value of 7.92 μM). Isobavachalcone also induces Reactive Oxyen Species (ROS) generation in OVCAR-8 cells and has exhibit cancer anti-promotive and anti-proliferative activity.

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Reinheit: 98.99%
CAS. Nr.: 20784-50-3
Formel: C₂₀H₂₀O₄
Molecular Weight:324.37

Speicherung:

4°C, protect from light

* In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

Isobavachalcone Chemical Structure

CAS. Nr.: 20784-50-3

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (10)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

IC50: 7.92 μM (OVCAR-8 cell)^[1]

Alle Akt Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>10 μM Compound: 8	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 25710081]
HCT-116	IC₅₀	75.48 μM Compound: 50	Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability	[PMID: 33445154]
HUVEC	IC₅₀	>100 μM Compound: 1c	Toxicity against HUVEC incubated for 48 hrs by MTT assay Toxicity against HUVEC incubated for 48 hrs by MTT assay	[PMID: 25590864]
K562	IC₅₀	>10 μM Compound: 8	Cytotoxicity against human K562 cells after 48 hrs by MTT assay Cytotoxicity against human K562 cells after 48 hrs by MTT assay	[PMID: 25710081]
K562	IC₅₀	29.49 μM Compound: 1c	Antitumor activity against human K562 cells incubated for 48 hrs by MTT assay Antitumor activity against human K562 cells incubated for 48 hrs by MTT assay	[PMID: 25590864]
OVCAR-8	IC₅₀	7.92 μM Compound: 60; IBC	Cytotoxicity against human OVCAR-8 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against human OVCAR-8 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 35151222]
PC-3	IC₅₀	15.06 μM Compound: 60; IBC	Cytotoxicity against human PC3 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against human PC3 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 35151222]
Platelet	IC₅₀	0.5 μM Compound: 1	Antiplatelet activity against rabbit platelet assessed as inhibition of arachidonic acid-induced platelet aggregation preincubated for 3 mins by turbidimetric method Antiplatelet activity against rabbit platelet assessed as inhibition of arachidonic acid-induced platelet aggregation preincubated for 3 mins by turbidimetric method	[PMID: 8759164]
Platelet	IC₅₀	41.6 μM Compound: 1	Antiplatelet activity against rabbit platelet assessed as inhibition of platelet-activating factor-induced platelet aggregation preincubated for 3 mins by turbidimetric method Antiplatelet activity against rabbit platelet assessed as inhibition of platelet-activating factor-induced platelet aggregation preincubated for 3 mins by turbidimetric method	[PMID: 8759164]
Platelet	IC₅₀	65.1 μM Compound: 1	Antiplatelet activity against rabbit platelet assessed as inhibition of collagen-induced platelet aggregation preincubated for 3 mins by turbidimetric method Antiplatelet activity against rabbit platelet assessed as inhibition of collagen-induced platelet aggregation preincubated for 3 mins by turbidimetric method	[PMID: 8759164]
Raji	IC₅₀	320 molar ratio Compound: 4	Inhibition of TPA-induced EBV-early antigen activation in human Raji cells Inhibition of TPA-induced EBV-early antigen activation in human Raji cells	[PMID: 16441065]
RAW264.7	IC₅₀	1.6 μM Compound: Table 4, R5C1	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells	[PMID: 32208222]
RAW264.7	IC₅₀	16.4 μM Compound: 2	Cytotoxicity against mouse RAW264.7 cells by MTT assay Cytotoxicity against mouse RAW264.7 cells by MTT assay	[PMID: 16643064]
RAW264.7	IC₅₀	3.5 μg/mL Compound: 133	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production	[PMID: 31255927]
RAW264.7	IC₅₀	3.5 μg/mL Compound: 157	Anti-inflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production pre-treated with compounds for 2 hrs followed by LPS-stimulation measured for 22 hrs by Griess reagent based analysis Anti-inflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production pre-treated with compounds for 2 hrs followed by LPS-stimulation measured for 22 hrs by Griess reagent based analysis	[PMID: 37683361]
RAW264.7	IC₅₀	6.4 μM Compound: 2	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite accumulation after 20 hrs Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite accumulation after 20 hrs	[PMID: 16643064]
SW480	IC₅₀	44.07 μM Compound: 50	Antiproliferative activity against human SW480 cells assessed as reduction in cell viability Antiproliferative activity against human SW480 cells assessed as reduction in cell viability	[PMID: 33445154]
THP-1	CC₅₀	35.95 μM Compound: 1	Cytotoxicity against human THP1 cells assessed as cell viability after 72 hrs by MTT assay Cytotoxicity against human THP1 cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 26906638]
Vero	CC₅₀	726.82 μg/mL Compound: 14, 4-hydroxy-isocordoin	Cytotoxicity against african green monkey Vero cells assessed as cell death after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells assessed as cell death after 72 hrs by MTT assay	[PMID: 22169259]
Vero	IC₅₀	31.25 μg/mL Compound: 14, 4-hydroxy-isocordoin	Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell growth after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell growth after 72 hrs by MTT assay	[PMID: 22169259]

In Vitro

Isobavachalcone (6.0-48.0 μM; 72 hours; OVCAR-8, PC3, A549, MCF-7, L-02 and HUVEC cells) treatment inhibits the proliferation of human cancer cells. Isobavachalcone inhibits PC3, A549, MCF-7, L-02 and HUVEC cells growth with IC₅₀ values of 15.06 μM, 32.2 μM, 28.29 μM, 31.61 μM and 31.3 μM, respectively^[1].
Isobavachalcone (0-18 μM; 6 hours; OVCAR-8 and PC3 cells) treatment results in a concentration-and time-dependent down-regulation of the Ser-473 phosphorylation of Akt and GSK3b phosphorylation^[1].
Isobavachalcone (0-18 μM; 72 hours; OVCAR-8 and PC3 cells) treatment causes apoptosis via caspase- and ROS-involved mitochondrial pathway^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

OVCAR-8, PC3, A549, MCF-7, L-02 and HUVEC cells
Concentration:

6.0-48.0 μM
Incubation Time:

72 hours
Result:

Inhibited the proliferation of human cancer cells.

Western Blot Analysis^[1]

Cell Line:

OVCAR-8 or PC3 cells
Concentration:

0 μM, 6 μM, 12 μM, and 18 μM
Incubation Time:

6 hours
Result:

A concentration-and time-dependent down-regulation of the Ser-473 phosphorylation of Akt. GSK3b phosphorylation was also inhibited in a concentration- and time-dependent manner.

Apoptosis Analysis^[1]

Cell Line:

OVCAR-8 cells and PC3 cells
Concentration:

0 μM, 6 μM, 12 μM, and 18 μM
Incubation Time:

72 hours
Result:

Led to dose dependent increase of apoptosis.

In Vivo

Isobavachalcone (20 mg/kg; intraperitoneal injection; for 0.5-24 hours; female Kunming mice) treatment results in an increase in blood glucose levels, reaching a maximum within 1 hour and maintaining until 4 hours post-dosing^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Seven-week-old specific pathogen-free female Kunming mice (18-22 g)^[1]
Dosage:

20 mg/kg
Administration:

Intraperitoneal injection; for 0.5, 1, 2, 4, 6, 8, 12, 24 hours
Result:

Increased in blood glucose levels.

Chemical Information

CAS. Nr. 20784-50-3
Appearance Solid
Molecular Weight 324.37
Formel C₂₀H₂₀O₄
Color Light yellow to yellow
SMILES

O=C(/C=C/C1=CC=C(C=C1)O)C2=CC=C(C(C/C=C(C)\C)=C2O)O
Synonyms

Corylifolinin; Isobacachalcone
Structure Classification
- Flavonoids
- Chalcones
- Phenols
- Polyphenols
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

Publications (10)

All Histological Imaging/StainingWBCell Proliferation/Viability Assay

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Journal Impact Factor

Acta Pharm Sin B

Chrysin serves as a novel inhibitor of DGK α/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC). [Abstract]

2021 Jan;11(1):143-155. PMID: 33532186
Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 Oct 15:489:144992. PMID: 40466530
J Ethnopharmacol

An integrated network pharmacology, molecular docking and experiment validation study to investigate the potential mechanism of Isobavachalcone in the treatment of osteoarthritis. [Abstract]

2024 May 23:326:117827. PMID: 38310989
J Ethnopharmacol

Discovery of bakuchiol as an AIM2 inflammasome activator and cause of hepatotoxicity. [Abstract]

2022 Nov 15:298:115593. PMID: 35973629
J Ethnopharmacol

Investigation of the mechanism of Isobavachalcone in treating rheumatoid arthritis through a combination strategy of network pharmacology and experimental verification. [Abstract]

2022 May 4;294:115342. PMID: 35525528
Food Funct

Isobavachalcone ameliorates cognitive deficits, and Aβ and tau pathologies in triple-transgenic mice with Alzheimer's disease. [Abstract]

2021 Sep 7;12(17):7749-7761. PMID: 34269361

Isobavachalcone purchased from MedChemExpress. Usage Cited in: Food Funct. 2021 Sep 7;12(17):7749-7761. [Abstract]

Representative images of the cortex by silver staining treated with Isobavachalcone (ISO) (5, 10 μg/mL).
Int Immunopharmacol

Isobavachalcone suppresses expression of inducible nitric oxide synthase induced by Toll-like receptor agonists. [Abstract]

2013 Jan;15(1):38-41. PMID: 23164691

Isobavachalcone purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2013 Jan;15(1):38-41. [Abstract]

IBC inhibits iNOS protein induced by TLRs agonists. A–C) RAW264.7 cells are pretreated with 20 or 50 μM IBC for 1 h and then further stimulated with MALP-2 (10 μg/mL) (A), LPS (10 μg/mL) (B), or poly[I:C] (10 μg/mL) (C) for an additional 8 h. Cell lysates are analyzed for iNOS and β-actin protein by immunoblots. Veh, vehicle; IBC, isobavachalcone.

Isobavachalcone purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2013 Jan;15(1):38-41. [Abstract]

RAW264.7 cells were treated with Isobavachalcone (IBC) (20, 50, 100 μM) for 4 h. Twenty microliters of the CellTiter 96 AQ_ueous One Solution Reagent was added directly to culture wells.
Chem Biol Interact

Bavachinin, a main compound of Psoraleae Fructus, facilitates GSDMD-mediated pyroptosis and causes hepatotoxicity in mice. [Abstract]

2024 Sep 1:400:111133. PMID: 38969277
ACS Chem Neurosci

Inhibitory Effects of Isobavachalcone on Tau Protein Aggregation, Tau Phosphorylation, and Oligomeric Tau-Induced Apoptosis. [Abstract]

2021 Jan 6;12(1):123-132. PMID: 33320518

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (308.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0829 mL

15.4145 mL

30.8290 mL

5 mM

0.6166 mL

3.0829 mL

6.1658 mL

10 mM

0.3083 mL

1.5414 mL

3.0829 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (6.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Jing H, et al. Abrogation of Akt signaling by Isobavachalcone contributes to its anti-proliferative effects towards human cancer cells. Cancer Lett. 2010 Aug 28;294(2):167-77. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.0829 mL	15.4145 mL	30.8290 mL	77.0725 mL
	5 mM	0.6166 mL	3.0829 mL	6.1658 mL	15.4145 mL
	10 mM	0.3083 mL	1.5414 mL	3.0829 mL	7.7072 mL
	15 mM	0.2055 mL	1.0276 mL	2.0553 mL	5.1382 mL
	20 mM	0.1541 mL	0.7707 mL	1.5414 mL	3.8536 mL
	25 mM	0.1233 mL	0.6166 mL	1.2332 mL	3.0829 mL
	30 mM	0.1028 mL	0.5138 mL	1.0276 mL	2.5691 mL
	40 mM	0.0771 mL	0.3854 mL	0.7707 mL	1.9268 mL
	50 mM	0.0617 mL	0.3083 mL	0.6166 mL	1.5414 mL
	60 mM	0.0514 mL	0.2569 mL	0.5138 mL	1.2845 mL
	80 mM	0.0385 mL	0.1927 mL	0.3854 mL	0.9634 mL
	100 mM	0.0308 mL	0.1541 mL	0.3083 mL	0.7707 mL

Keywords

Isobavachalcone

10 Cited Publications

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Isobavachalcone

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Publications Citing Use of MedChemExpress (MCE) Isobavachalcone

Alle Akt Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (10)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Isobavachalcone Related Classifications

HY-13065 Related Classifications