Cat. No.: HY-B0900

Anethole

(Synonyms: Anise camphor; p-Propenylanisole; Isoestragole)

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

Anethole is a type of orally active aromatic compound that is widely found in nature and used as a flavoring agent. Anethole possesses anticancer, anti-inflammatory, antioxidant, antibacterial, antifungal, anesthetic, estrogenic, central nervous system depressant, hypnotic, insecticidal, and gastroprotective effects. Anethole can be used in the study of oxidative stress-related skin diseases and prostate cancer.

For research use only. We do not sell to patients.

Purity: 99.81%
CAS No.: 104-46-1
Formula: C₁₀H₁₂O
Molecular Weight:148.21

Storage:

Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Anethole Chemical Structure

CAS No.: 104-46-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

MMP-9

All NF-κB Isoforms :

All MMP Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
MG-63	GI₅₀	60.25 μM Compound: 32	Antiproliferative activity against human MG-63 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human MG-63 cells assessed as growth inhibition by MTT assay	[PMID: 36858050]

In Vitro

Anethole (0.5-1 μM; 1 h) can inhibit H2O2 (300 Μm; 24 h)-induced apoptosis and viability loss in human skin fibroblasts CRL1474^[2].
Anethole has synergistic effects on the antifungal activities of phytochemicals including polygodial and 2E-undecenal against Saccharomyces cerevisiae and Candida albicans^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

CRL1474 (human skin fibroblasts)
Concentration:

0.5 µM, 1 µM, 10 µM. After H₂O₂treatment (300 μM; 24 h)
Incubation Time:

1 h
Result:

Inhibited H2O2-induced cytotoxicity.

In Vivo

Anethole (62.5-500 mg/kg; Intraperitoneal injection, single dose) has an anti-inflammatory effect in BALB/C mice induced by LPS (1.5 mg/kg) (HY-D1056)^[4].
Anethole (250-1000 mg/kg; P.O.; Once every other day for 60 consecutive days) has anti-tumor effects in Ehrlich ascites tumor Swiss albino mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/C mice model induced by LPS^[4]
Dosage:

62.5 mg/kg, 125 mg/kg, 250 mg/kg, 500 mg/kg
Administration:

Intraperitoneal injection (i.p.), single dose. After LPS treatment (1.5 mg/kg; intratracheal injection, single dose).
Result:

Decreased total protein concentration.
Decreased numbers of inflammatory cells including neutrophils and macrophages.
Decreased the inflammatory mediators MMP-9, TNF-α, NO.
Decreased LPS-induced histopathological changes.
Suppressed the activation of NF-κB by blocking IκB-α degradation.

Animal Model:

Ehrlich ascites tumour (ETC) Swiss albino mouse model^[5]
Dosage:

250 mg/kg; 500 mg/kg; 1000 mg/kg
Administration:

Oral gavage (p.o.); Once every other day for 60 consecutive days. After implantation of ETC cells (2.5×10⁶ cells/mouse) into the right hind paw muscle of mice.
Result:

Reduced tumor weight and volume in mice.
Prolonged the survival time of ETC mice.

Chemical Information

CAS No. 104-46-1
Appearance <20°C Solid,>21°C Liquid
Molecular Weight 148.21
Formula C₁₀H₁₂O
Color Colorless to off-white
SMILES

C/C=C/C1=CC=C(OC)C=C1
Synonyms

Anise camphor; p-Propenylanisole; Isoestragole
Structure Classification
- Phenylpropanoids
- Simple Phenylpropanols
Initial Source
- Plants
- Umbelliferae
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (2)

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Journal Impact Factor

Int Immunopharmacol

Anethole inhibits RANKL-induced osteoclastogenesis by downregulating ERK/AKT signaling and prevents ovariectomy-induced bone loss in vivo. [Abstract]

2021 Nov:100:108113. PMID: 34530203
Toxicol In Vitro

Inhibitory potential of phytochemicals on species-specific breast cancer resistance protein transport activity. [Abstract]

2025 Dec:109:106128. PMID: 40796067

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (674.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

6.7472 mL

33.7359 mL

67.4718 mL

5 mM

1.3494 mL

6.7472 mL

13.4944 mL

10 mM

0.6747 mL

3.3736 mL

6.7472 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (16.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (16.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (16.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Freire R S, et al. Synthesis and antioxidant, anti-inflammatory and gastroprotector activities of anethole and related compounds[J]. Bioorganic & medicinal chemistry, 2005, 13(13): 4353-4358. [Content Brief]

[2]. Galicka A, Krętowski R, Nazaruk J, et al. Anethole prevents hydrogen peroxide-induced apoptosis and collagen metabolism alterations in human skin fibroblasts[J]. Molecular and Cellular Biochemistry, 2014, 394: 217-224. [Content Brief]

[3]. Fujita K, et al. Anethole, a potential antimicrobial synergist, converts a fungistatic dodecanol to a fungicidal agent[J]. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 2007, 21(1): 47-51. [Content Brief]

[4]. Kang P, et al. Anti-inflammatory effects of anethole in lipopolysaccharide-induced acute lung injury in mice[J]. Life sciences, 2013, 93(24): 955-961. [Content Brief]

[5]. Al-Harbi M M, et al. Influence of anethole treatment on the tumour induced by Ehrlich ascites carcinoma cells in paw of Swiss albino mice[J]. European Journal of Cancer Prevention, 1995, 4(4): 307-318. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	6.7472 mL	33.7359 mL	67.4718 mL	168.6796 mL
	5 mM	1.3494 mL	6.7472 mL	13.4944 mL	33.7359 mL
	10 mM	0.6747 mL	3.3736 mL	6.7472 mL	16.8680 mL
	15 mM	0.4498 mL	2.2491 mL	4.4981 mL	11.2453 mL
	20 mM	0.3374 mL	1.6868 mL	3.3736 mL	8.4340 mL
	25 mM	0.2699 mL	1.3494 mL	2.6989 mL	6.7472 mL
	30 mM	0.2249 mL	1.1245 mL	2.2491 mL	5.6227 mL
	40 mM	0.1687 mL	0.8434 mL	1.6868 mL	4.2170 mL
	50 mM	0.1349 mL	0.6747 mL	1.3494 mL	3.3736 mL
	60 mM	0.1125 mL	0.5623 mL	1.1245 mL	2.8113 mL
	80 mM	0.0843 mL	0.4217 mL	0.8434 mL	2.1085 mL
	100 mM	0.0675 mL	0.3374 mL	0.6747 mL	1.6868 mL

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Anethole

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Publications Citing Use of MedChemExpress (MCE) Anethole

All NF-κB Isoforms

All MMP Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References