ATTO 740 NHS ester

nude mice model (8-week-old female, Charles River)^[1]

200 μL of 6×10^-5 M A740-R₀₁

i.v.; single dose

Produced a 2-fold photoacoustic signal increase (1.87 arbitrary units vs. baseline 0.99 arbitrary units) in integrin αvβ₆-positive A431 xenografts 1 h after injection.
Produced an 8-fold fluorescence signal increase (8.27 arbitrary units vs. baseline 1.01 arbitrary units) in integrin αvβ₆-positive A431 xenografts 1 h after injection.
Showed no significant photoacoustic signal changes (pre-injection: 0.96 arbitrary units; 1 h post-injection: 1.07 arbitrary units) in integrin αvβ₆-negative 293T xenografts.
Showed no significant fluorescence signal changes (pre-injection: 1.00 arbitrary units; 1 h post-injection: 1.10 arbitrary units) in integrin αvβ₆-negative 293T xenografts.
Exhibited the highest maximum radiance in the liver, followed by the intestines, spleen, and kidneys in ex vivo fluorescence imaging 1 h after injection.
Detected increased photoacoustic signal in the liver at 1 h after injection, temporally dependent increases in intestinal signals peaking at 4 h, and minimal renal contrast throughout the study.