ATTO 740 NHS ester 

ATTO 740 NHS ester is a near-infrared fluorescent dye and a multimodal fluorescence/photoacoustic contrast agent with excellent near-infrared emission properties and extremely high photostability. The photoacoustic signal of ATTO 740 NHS ester shows no significant decrease after continuous irradiation with a 750 nm laser for 30 min, making it suitable for in vivo fluorescence imaging and photoacoustic contrast imaging. When conjugated with the cystine knot peptide R01, ATTO 740 NHS ester enables precise detection of integrin αvβ6-positive cells and tumors in nude mouse xenograft models^[1].

ATTO 740 NHS ester (10 mg/mL; up to 1 h) successfully conjugates to the N-terminal amine of R₀1 peptide to form A740-R₀1, which binds integrin αᵥβ₆ with a IC₅₀ of 39.4 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

The A740-R₀₁ conjugate (200 μL of 6×10^-5 M; i.v.; single dose), synthesized using ATTO 740 NHS ester, enables specific detection of integrin αvβ₆-positive tumors, producing a 2-fold photoacoustic signal increase and 8-fold fluorescence signal increase in positive xenografts relative to baseline 1 h after injection^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1448146-89-1

[ATTO 740 NHS ester]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Zhang C, et al. A Cystine Knot Peptide Targeting Integrin αvβ6 for Photoacoustic and Fluorescence Imaging of Tumors in Living Subjects. J Nucl Med. 2016;57(10):1629-1634.