BRD4 Inhibitor-30

Cat. No.: HY-160499 Purity: 99.89%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

BRD4 inhibitor-30 (Compound 1) is a BRD4 inhibitor with a IC₅₀ value of 415 nM.

For research use only. We do not sell to patients.

BRD4 Inhibitor-30 Chemical Structure

CAS No. : 1789731-20-9

Size	Price	Stock	Quantity
1 mg	USD 400	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 1000	In-stock	Estimated Time of Arrival: December 31
10 mg		Get quote
50 mg		Get quote

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Purity & Documentation
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Description

BRD4 inhibitor-30 (Compound 1) is a BRD4 inhibitor with a IC₅₀ value of 415 nM^[1].

Molecular Weight

522.64

Formula

C₂₈H₃₈N₆O₄

CAS No.

1789731-20-9

Appearance

Solid

Color

White to off-white

SMILES

CC[C@H]1N(C2CCCC2)C3=NC(OC4=C(C=C(C=C4)C(NC5CCN(CC5)C)=O)OC)=NC=C3N(C1=O)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (191.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9134 mL	9.5668 mL	19.1336 mL
5 mM	0.3827 mL	1.9134 mL	3.8267 mL
10 mM	0.1913 mL	0.9567 mL	1.9134 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (4.78 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (4.78 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (4.78 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.89%

Select Batch:

Data Sheet (270 KB) SDS (0 KB)

COA (248 KB) HNMR (140 KB) RP-HPLC (139 KB) LCMS (204 KB)

Handling Instructions (2659 KB)

References

[1]. Koblan LW, et al. Assessment of Bromodomain Target Engagement by a Series of BI2536 Analogues with Miniaturized BET-BRET. ChemMedChem. 2016 Dec 6;11(23):2575-2581. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9134 mL	9.5668 mL	19.1336 mL	47.8341 mL
	5 mM	0.3827 mL	1.9134 mL	3.8267 mL	9.5668 mL
	10 mM	0.1913 mL	0.9567 mL	1.9134 mL	4.7834 mL
	15 mM	0.1276 mL	0.6378 mL	1.2756 mL	3.1889 mL
	20 mM	0.0957 mL	0.4783 mL	0.9567 mL	2.3917 mL
	25 mM	0.0765 mL	0.3827 mL	0.7653 mL	1.9134 mL
	30 mM	0.0638 mL	0.3189 mL	0.6378 mL	1.5945 mL
	40 mM	0.0478 mL	0.2392 mL	0.4783 mL	1.1959 mL
	50 mM	0.0383 mL	0.1913 mL	0.3827 mL	0.9567 mL
	60 mM	0.0319 mL	0.1594 mL	0.3189 mL	0.7972 mL
	80 mM	0.0239 mL	0.1196 mL	0.2392 mL	0.5979 mL
	100 mM	0.0191 mL	0.0957 mL	0.1913 mL	0.4783 mL