Amlodipine
Based on 15 publication(s) in Google Scholar
Amlodipine, an antianginal agent and an orally active dihydropyridine calcium channel blocker, works by blocking the voltage-dependent L-type calcium channels, thereby inhibiting the initial influx of calcium. Amlodipine can be used for the research of high blood pressure and cancer.
For research use only. We do not sell to patients.
- Purity: 99.87%
- CAS No.: 88150-42-9
- Formula: C20H25ClN2O5
- Molecular Weight:408.88
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Publications Citing Use of MedChemExpress (MCE) Amlodipine
More- J Adv Res. 2024 Aug:62:187-198. [Abstract]
- Exp Mol Med. 2021 Apr;53(4):572-590. [Abstract]
- Sci Adv. 2025 Jan 24;11(4):eadp4765. [Abstract]
- Br J Pharmacol. 2022 May;179(9):2054-2077. [Abstract]
- Cell Rep. 2026 May 28;45(6):117471. [Abstract]
- Acta Physiol. 2026 Apr;242(4):e70189. [Abstract]
- Cells. 2022 Oct 8;11(19):3156. [Abstract]
- Food Chem Toxicol. 2025 Feb 10:115317. [Abstract]
- J Biochem Mol Toxicol. 2022 Oct 7;e23238. [Abstract]
- J Chem Thermodyn. 2021, 106495.
- Biochem Biophys Res Commun. 2020 Feb 19;522(4):862-868. [Abstract]
- Nat Prod Commun. 2024 Mar 20.
- Dissolut Technol. 2021 Jun.
- SSRN. 2025 Nov 6.
- medRxiv. 2025 May 21.
Customer Validation & Images
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Cell Proliferation/Viability Assay
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Flow Cytometry
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Cell Imaging/Staining
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In Vivo Efficacy Study
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Histological Imaging/Staining
All Calcium Channel Isoforms
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Biological Activity
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L-type calcium channel |
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Cell Line
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Type | Value | Description | References |
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| LLC-MK2 | IC50 |
70.82 μM
Compound: Amlodipine
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Cytotoxicity against rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay
Cytotoxicity against rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay
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[PMID: 20934347] |
| LLC-MK2 | IC50 |
8.07 μM
Compound: Amlodipine
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Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi infected in rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay
Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi infected in rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay
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[PMID: 20934347] |
| LLC-PK1 | IC50 |
22 μM
Compound: Amlodipine
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TP_TRANSPORTER: inhibition of Daunorubicin transepithelial transport (basal to apical) (Daunorubicin: 0.035 uM) in MDR1-expressing LLC-PK1 cells
TP_TRANSPORTER: inhibition of Daunorubicin transepithelial transport (basal to apical) (Daunorubicin: 0.035 uM) in MDR1-expressing LLC-PK1 cells
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[PMID: 11145223] |
| Ventricular myocyte | IC50 |
0.57 μM
Compound: Amlodipine
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Inhibition of L-type calcium channel measured using whole-cell patch clamp in guinea pig ventricular myocytes
Inhibition of L-type calcium channel measured using whole-cell patch clamp in guinea pig ventricular myocytes
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[PMID: 22761000] |
Amlodipine (20-40 μM; 48 h) reduces BrdU incorporation to 68.6% and 26.3% at concentrations of 20 and 30 μM in A431 cells, respectively[3].
Amlodipine (30 μM; pretreated for 1 h) significantly attenuates the uridine 5′-triphosphate (UTP)-induced increases of [Ca2+]i in A431 cells[3].
Amlodipine (30 μM) inhibits the store-operated Ca2+influx evoked by Thapsigargin in Fluo-3-loaded cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Amlodipine (10 mg/kg; i.p. once daily for 20 days) causes a significant retardation of tumor growth and prolongs the survival of A431 tumor-bearing mice[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:ATP2B1loxP/loxP mice[4]
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Dosage:5 mg/kg/day
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Administration:Subcutaneously implanted osmotic pump for 2 weeks
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Result:Significantly decreased the blood pressure.
Chemical Information
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CAS No. 88150-42-9
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Appearance Solid
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Molecular Weight 408.88
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Formula C20H25ClN2O5
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Color White to off-white
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SMILES
O=C(OCC)C1=C(NC(C)=C(C1C2=CC=CC=C2Cl)C(OC)=O)COCCN
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (15)
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Journal Impact Factor
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Most Recent
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J Adv Res
Calcium Influx: An Essential Process by which α-Synuclein Regulates Morphology of Erythrocytes. [Abstract]2024 Aug:62:187-198. PMID: 37714326 -
Exp Mol Med
Sublytic C5b-9 induces glomerular mesangial cell proliferation via ERK1/2-dependent SOX9 phosphorylation and acetylation by enhancing Cyclin D1 in rat Thy-1 nephritis. [Abstract]2021 Apr;53(4):572-590. PMID: 33811247
Amlodipine purchased from MedChemExpress. Usage Cited in: Exp Mol Med. 2021 Apr;53(4):572-590. [Abstract]
Rat GMCs pretreated with the calcium channel blocker Amlodipine (10μM), inhibitors of PKC (Go6983; 10μM), c-Raf (AZ628, 10μM) and MEK1/2 (U0126, 10μM) for 30min were stimulated with sublytic C5b-9 for 4 0min or 2h. The intracellular calcium was determined by Fluo-4 AM. The results showed that Amlodipine effectively blocked the rise in intracellular calcium levels in rat GMCs induced by 40 min of sublytic C5b-9 stimulation.
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Sci Adv
WWC proteins-mediated compensatory mechanism restricts schwannomatosis driven by NF2 loss of function. [Abstract]2025 Jan 24;11(4):eadp4765. PMID: 39841844 -
Br J Pharmacol
Amlodipine, an anti-hypertensive drug, alleviates non-alcoholic fatty liver disease by modulating gut microbiota. [Abstract]2022 May;179(9):2054-2077. PMID: 34862599 -
Cell Rep
2026 May 28;45(6):117471. PMID: 42213774 -
Acta Physiol
Tacrolimus Induced Hypertension and Vascular Remodeling Includes Mechanisms of Cellular Senescence-The Protective Effect of Valsartan. [Abstract]2026 Apr;242(4):e70189. PMID: 41814130 -
Cells
Calcium-Related Genes Predicting Outcomes and Serving as Therapeutic Targets in Endometrial Cancer. [Abstract]2022 Oct 8;11(19):3156. PMID: 36231119 -
Food Chem Toxicol
Coexposure to fluoride and sulfur dioxide aggravates enamel mineralization disorders in mice by disrupting calcium homeostasis-mediated endoplasmic reticulum stress. [Abstract]2025 Feb 10:115317. PMID: 39938609
Amlodipine purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2025 Feb 10:115317. [Abstract]
A CCK-8 assay was used to detect the effect of AMs on the proliferation of Ishikawa cells. The results showed that the half-maximal inhibitory concentration (IC50) of Amlodipine (AM) against Ishikawa cells (at 72 h) was 12.36 μM.
Amlodipine purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2025 Feb 10:115317. [Abstract]
Cell apoptosis rate was detected by flow cytometry using Annexin-V/PI staining. The results showed that the apoptotic rate of the Amlodipine (AM) (15 μM; 12 h)-treated Ishikawa cells was 16.8%, which was 2.16-fold higher than that in cells treated with DMSO.
Amlodipine purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2025 Feb 10:115317. [Abstract]
EdU staining showing the effect of Amlodipine (AM) (15 μM; 48 h) on Ishikawa cells. The EdU staining assay revealed that the EdU-positive rate of the AM-treated Ishikawa cells was significantly reduced compared with that of the control group.
Amlodipine purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2025 Feb 10:115317. [Abstract]
Amlodipine (AM) (15 or 20 mg/kg; i.p.; every other day for two weeks) inhibited tumor growth in a dose-dependent manner in BALB/c nude mice bearing subcutaneous xenografts established from Ishikawa cells.
Amlodipine purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2025 Feb 10:115317. [Abstract]
H&E and PCNA staining of sections from mice treated with Amlodipine (AM) (15 or 20 mg/kg; i.p.; every other day for two weeks).
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J Biochem Mol Toxicol
An in vivo and in vitro model on the protective effect of cilnidipine on contrast-induced nephropathy via regulation of apoptosis and CaMKⅡ/mPTP pathway. [Abstract]2022 Oct 7;e23238. PMID: 36207783 -
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Biochem Biophys Res Commun
Combinatorial screening of a panel of FDA-approved drugs identifies several candidates with anti-Ebola activities. [Abstract]2020 Feb 19;522(4):862-868. PMID: 31806372 -
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Solvent & Solubility
DMSO : 31.25 mg/mL (76.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3 mg/mL (7.34 mM); Clear solution
This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 3 mg/mL (7.34 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 3 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (889 KB)
- English - EN (889 KB)
- Français - FR (889 KB)
- Deutsch - DE (889 KB)
- Norwegian - NO (889 KB)
- Español - ES (889 KB)
- Swedish - SV (889 KB)
- Italian - IT (889 KB)
- Korean - KR (889 KB)
- Portuguese - PT (889 KB)
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Handling Instructions (2659 KB)
References
[1]. Kishen G. Bulsara, et al. Amlodipine.
[2]. Haria M, et al. Amlodipine. A reappraisal of its pharmacological properties and therapeutic use in cardiovascular disease [published correction appears in Drugs 1995 Nov;50(5):896]. Drugs. 1995;50(3):560-586. [Content Brief]
[3]. Yoshida J, et, al. Antitumor effects of amlodipine, a Ca2+ channel blocker, on human epidermoid carcinoma A431 cells in vitro and in vivo. Eur J Pharmacol. 2004 May 25;492(2-3):103-12. [Content Brief]
[4]. Okuyama Y, et, al. The effects of anti-hypertensive drugs and the mechanism of hypertension in vascular smooth muscle cell-specific ATP2B1 knockout mice. Hypertens Res. 2018 Feb;41(2):80-87. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4457 mL | 12.2285 mL | 24.4571 mL | 61.1426 mL |
| 5 mM | 0.4891 mL | 2.4457 mL | 4.8914 mL | 12.2285 mL | |
| 10 mM | 0.2446 mL | 1.2229 mL | 2.4457 mL | 6.1143 mL | |
| 15 mM | 0.1630 mL | 0.8152 mL | 1.6305 mL | 4.0762 mL | |
| 20 mM | 0.1223 mL | 0.6114 mL | 1.2229 mL | 3.0571 mL | |
| 25 mM | 0.0978 mL | 0.4891 mL | 0.9783 mL | 2.4457 mL | |
| 30 mM | 0.0815 mL | 0.4076 mL | 0.8152 mL | 2.0381 mL | |
| 40 mM | 0.0611 mL | 0.3057 mL | 0.6114 mL | 1.5286 mL | |
| 50 mM | 0.0489 mL | 0.2446 mL | 0.4891 mL | 1.2229 mL | |
| 60 mM | 0.0408 mL | 0.2038 mL | 0.4076 mL | 1.0190 mL |
Amlodipine Related Classifications
HY-B0317 Related Classifications
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