CD28-IN-3
CD28-IN-3 is a CD28 inhibitor with an IC50 of 7.80 μM and a Kd of 52.45 μM. CD28-IN-3 selectively blocks the CD28-B7 interaction, thereby inhibiting CD28-mediated costimulation. CD28-IN-3 suppresses the production of proinflammatory cytokines IFN-γ, IL-2 and TNF-α. CD28-IN-3 can be used in the research of checkpoint-resistant cancers.
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- CAS No.: 850550-70-8
- Formule: C17H24N4OS2
- Masse moléculaire:364.53
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
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Activité biologique
Description
In Vitro
CD28-IN-3 (Compound 22VS) (0-100 μM; 2 h) inhibits the interactions of membrane-bound CD28-CD80 and CD28-CD86 in CHO-K1 cells, with corresponding submicromolar IC50 values of 1.48 μM and 1.39 μM, respectively[1].
CD28-IN-3 (10-300 μM; 24 h) shows no cytotoxicity against human Jurkat T lymphocytes even at concentrations up to 300 μM following a 24 h incubation[1].
CD28-IN-3 (0-200 μM; 5 h) inhibits CD28-mediated T cell costimulation in the Jurkat-Raji co-culture system, with an IC50 of 6.96 μM[1].
CD28-IN-3 (10-50 μM; 48 h) inhibits the release of CD28-dependent proinflammatory cytokines (IFN-γ, IL-2, TNF-α) in a dose-dependent manner in 3D A549 tumor-PBMC co-culture systems and human PBMC-mucosa co-culture systems[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Human Jurkat T lymphocytes
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Concentration:10, 30, 100 and 300 μM
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Incubation Time:24 h (37°C)
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Result:Maintained cell viability above 85% at all tested concentrations, showing no measurable cytotoxicity.
Chemical Information
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CAS No. 850550-70-8
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Masse moléculaire 364.53
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Formule C17H24N4OS2
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SMILES
CCC(NC(CSC1=NN=C(S1)NC2=CC=C(C=C2)C(C)C)=O)C
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
CD28-IN-3 Related Classifications
HY-181507 Related Classifications
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