Art. -Nr.: HY-N2229

Rhapontigenin

3 Cited Publications

Kundenbewertung

Based on 3 publication(s) in Google Scholar

Rhapontigenin is a natural analog of resveratrol with anticancer, antioxidant, antifungal and antibacterial activities. Rhapontigenin is amechanism-based, potent and selective cytochrome P450 1A1 inactivator (IC₅₀ = 400 nM). Rhapontigenin exhibits 400-fold and 23-fold selectivity for P450 1A1 over P450 1A2 and P450 1B1, respectively.

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Reinheit: 99.66%
CAS. Nr.: 500-65-2
Formel: C₁₅H₁₄O₄
Molecular Weight:258.27

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Rhapontigenin Chemical Structure

CAS. Nr.: 500-65-2

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target^[1]

CYP1A1

400 nM (IC₅₀)

Alle Cytochrome P450 Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	48 μM Compound: 10a	In vitro inhibitory concentration against proliferation of HL60 cells In vitro inhibitory concentration against proliferation of HL60 cells	[PMID: 12877593]
J774	IC₅₀	102.8 μM Compound: 6	Antiinflammatory activity in mouse J774 cells assessed as reduction in LPS-induced nitric oxide production after 24 hrs by Griess assay Antiinflammatory activity in mouse J774 cells assessed as reduction in LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 29726680]
J774	IC₅₀	19.4 μM Compound: 6	Antiinflammatory activity in mouse J774 cells assessed as reduction in LPS-induced MCP1 expression after 24 hrs by ELISA Antiinflammatory activity in mouse J774 cells assessed as reduction in LPS-induced MCP1 expression after 24 hrs by ELISA	[PMID: 29726680]
J774	IC₅₀	99.1 μM Compound: 6	Antiinflammatory activity in mouse J774 cells assessed as reduction in LPS-induced IL6 expression after 24 hrs by ELISA Antiinflammatory activity in mouse J774 cells assessed as reduction in LPS-induced IL6 expression after 24 hrs by ELISA	[PMID: 29726680]

In Vitro

Rhapontigenin (0-250 μM; 24 hours) demonstrates concentration-dependent?anti-cancer?activity with an IC₅₀ 115μM in HEP G2 cells^[1].
? Rhapontigenin (20 μM; 20 hours) pre-treatment decreases TGF-β triggered increased snail expression in diverse cancer cells^[2].
? Rhapontigenin (0-20 μM; 6 hours) inhibits TGF-β-induced expression of N-cadherin, vimentin, and CA9 in a dose-dependent manner^[2].
? Rhapontigenin inhibits ADP- and collagen-induced platelet aggregation with IC₅₀ values of 4 and 70 μg/ml, respectively^[3].
? Rhapontigenin demonstrates a strong inhibitory activity on the 13-hexosaminidase release induced by DNP-BSA, it exhibits IC₅₀ value of 0.03 mM in RBL 2H3 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:

HeLa, A549,769-P cells
Concentration:

0 μM; 2.5 μM; 5 μM; 10 μM; 20 μM
Incubation Time:

6 hours
Result:

Induced ubiquitination and degradation of HIF-1α.

In Vivo

Rhapontigenin (intraperitoneal injection; 25mg/kg) shows significant protection from death due to pulmonary thrombosis in mice, those samples are orally administered 90 min before tail vein injection of epinephrine and collagen^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

ICR mice^[3]
Dosage:

25mg/kg
Administration:

25mg/kg; intraperitoneal injection
Result:

Showed anti-thrombosis activity with 60% protection.

Chemical Information

CAS. Nr. 500-65-2
Appearance Solid
Molecular Weight 258.27
Formel C₁₅H₁₄O₄
Color Light yellow to yellow
SMILES

OC1=CC(/C=C/C2=CC=C(OC)C(O)=C2)=CC(O)=C1
Structure Classification
- Phenols
- Polyphenols
- Stilbenes
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (3)

All WBHistological Imaging/StainingIFRT-PCRIHC

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Journal Impact Factor

Nat Biomed Eng

Amelioration of Alzheimer's disease pathology by mitophagy inducers identified via machine learning and a cross-species workflow. [Abstract]

2022 Jan;6(1):76-93. PMID: 34992270

Rhapontigenin purchased from MedChemExpress. Usage Cited in: Nat Biomed Eng. 2022 Jan;6(1):76-93. [Abstract]

Effects of Kaem and Rhapontigenin (Rhap) (20, 40, 80 μM) on the protein levels of mitofusin2 (MFN2), YFP-Parkin and Tim23 in HeLa cells stably overexpressing YFP-Parkin.

Rhapontigenin purchased from MedChemExpress. Usage Cited in: Nat Biomed Eng. 2022 Jan;6(1):76-93. [Abstract]

Images showing co-localization of mitochondria (Mito-GFP) and lysosomes (LAMP1 antibody) under Kaem and Rhapontigenin (Rhap) (20 uM, 24 h) administration in GFP-mito-mCherry-Parkin HeLa cells.

Rhapontigenin purchased from MedChemExpress. Usage Cited in: Nat Biomed Eng. 2022 Jan;6(1):76-93. [Abstract]

Effects of Kaem and Rhapontigenin (Rhap) (0.2 mM) on designated gene expression in day 1 adult worms.

Rhapontigenin purchased from MedChemExpress. Usage Cited in: Nat Biomed Eng. 2022 Jan;6(1):76-93. [Abstract]

Immunohistochemical analysis of Aβ load in 3xTg AD mice hippocampi and cortices under Kaem or Rhapontigenin (Rhap) (100 mg/kg, p.o.) treatment.
Int Immunopharmacol

Rhapontigenin inhibits inflammation and senescence of chondrocytes from patients with osteoarthritis by targeting INHBA. [Abstract]

2026 Jan 1;168(Pt 1):115808. PMID: 41218586
FASEB J

Epigenetic mechanisms mediate cytochrome P450 1A1 expression and lung endothelial injury caused by MRSA in vitro and in vivo. [Abstract]

2024 Nov 30;38(22):e70205. PMID: 39588951

Rhapontigenin purchased from MedChemExpress. Usage Cited in: FASEB J. 2024 Nov 30;38(22):e70205. [Abstract]

Lung tissue H&E staining treated with Rhapontigenin (Rhap) (10 mg/kg, i.p.).

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 125 mg/mL (483.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.8719 mL

19.3596 mL

38.7192 mL

5 mM

0.7744 mL

3.8719 mL

7.7438 mL

10 mM

0.3872 mL

1.9360 mL

3.8719 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (8.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (8.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Roupe KA, et al. Preparative enzymatic synthesis and HPLC analysis of rhapontigenin: applications to metabolism, pharmacokinetics and anti-cancer studies.J Pharm Pharm Sci. 2005 Aug 22;8(3):374-86. [Content Brief]

[2]. Yeh YH, et al. Rhapontigenin inhibits TGF-β-mediated epithelial‑mesenchymal transition via the PI3K/AKT/mTOR pathway and is not associated with HIF-1α degradation.Oncol Rep. 2016 May;35(5):2887-95. [Content Brief]

[3]. Park EK, et al. Antithrombotic and antiallergic activities of rhaponticin from Rhei Rhizoma are activated by human intestinal bacteria.Arch Pharm Res. 2002 Aug;25(4):528-33. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.8719 mL	19.3596 mL	38.7192 mL	96.7979 mL
	5 mM	0.7744 mL	3.8719 mL	7.7438 mL	19.3596 mL
	10 mM	0.3872 mL	1.9360 mL	3.8719 mL	9.6798 mL
	15 mM	0.2581 mL	1.2906 mL	2.5813 mL	6.4532 mL
	20 mM	0.1936 mL	0.9680 mL	1.9360 mL	4.8399 mL
	25 mM	0.1549 mL	0.7744 mL	1.5488 mL	3.8719 mL
	30 mM	0.1291 mL	0.6453 mL	1.2906 mL	3.2266 mL
	40 mM	0.0968 mL	0.4840 mL	0.9680 mL	2.4199 mL
	50 mM	0.0774 mL	0.3872 mL	0.7744 mL	1.9360 mL
	60 mM	0.0645 mL	0.3227 mL	0.6453 mL	1.6133 mL
	80 mM	0.0484 mL	0.2420 mL	0.4840 mL	1.2100 mL
	100 mM	0.0387 mL	0.1936 mL	0.3872 mL	0.9680 mL

Keywords

Rhapontigenin

3 Cited Publications

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Rhapontigenin

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Publications Citing Use of MedChemExpress (MCE) Rhapontigenin

Alle Cytochrome P450 Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (3)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Rhapontigenin Related Classifications

HY-N2229 Related Classifications