Cat. No.: HY-A0210

Cerulenin

12 Cited Publications

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Based on 12 publication(s) in Google Scholar

Cerulenin, a potent, natural inhibitor of fatty acid synthase (FASN), is an epoxide produced by the fungus Cephalosporium caeruleus. Cerulenin inhibits topoisomerase I catalytic activity and augments SN-38-induced apoptosis. Cerulenin has antifungal and antitumor activies.

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Pureté: 98.90%
CAS No.: 17397-89-6
Formule: C₁₂H₁₇NO₃
Masse moléculaire:223.27

Stockage:

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Cerulenin Chemical Structure

CAS No.: 17397-89-6

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Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (12)
Solvant et solubilité
- In Vitro
- In Vivo
Protocole
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target

Fatty acid synthase (FASN)^[1]

Voir tous les produits spécifiques à Isoform Antibiotic :

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	21.86 μM Compound: 7	Inhibition of FASN in human A375 cells Inhibition of FASN in human A375 cells	[PMID: 21726077]
T-24	IC₅₀	17 μM Compound: cerulenin	Inhibition of human T24 cell proliferation Inhibition of human T24 cell proliferation	[PMID: 10585203]
ZR-75-1	IC₅₀	0.5 μg/mL Compound: Cerulenin	Inhibition of FAS in human ZR-75-1 cells by spectrophotometry Inhibition of FAS in human ZR-75-1 cells by spectrophotometry	[PMID: 12932120]

In Vitro

Cerulenin covalently binds to the catalytic site of FAS and disrupts the condensation reaction of acetyl-COA and malonyl-COA, inhibiting the biosynthesis of fatty acids and sterols in yeast. The Flavonoids quercetin and trans-Chalcone are effective against T. rubrum, with MICs of 125 and 7.5 μg/mL for the wild-type strain (MYA3108) and of 63 and 1.9 μg/mL for the ABC transporter mutant strain (ΔTruMDR2), respectively. The MICs of the Fluconazole and Cerulenin controls are 63 and 125 μg/mL for the wild-type strain and 30 and 15 μg/mL for the mutant strain, respectively^[1]. To explore the underlying mechanism of Steroidogenic acute regulatory protein (StAR)’s protective effect on endothelial dysfunction model, the inhibitor of fatty acid synthase and HMG-CoA reductase, Cerulenin (5 μg/mL) and Lovastatin, are used before palmitic acid (PA) added. The mRNA expression of IL-1β, TNFα, VCAM-1 and IL-6 are reduced while NO production is recovered with inhibitor treatment^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Cerulenin treatment of ob/ob mice has obvious effects on body weight. With 2 days of treatment, body weight in treated mice is decreased compared to a 5.7% weight gain in the controls. With prolonged (7 days) treatment, no body weight loss is observed, but body weight gain is slowed. In all groups, 60 mg/kg of Cerulenin is more effective than 30 mg/kg in inhibiting weight gain. If given daily or every other day, ATP content are increased 58.1% and 61.5% respectively by 7-day treatment of 60 mg/kg Cerulenin. Significant ATP elevation is also observed with only 2 days of treatment with 60 mg/kg Cerulenin. In contrast, 30 mg/kg Cerulenin, given either 2 or 7 days, does not show any significant effect on cellular ATP content^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 17397-89-6
Appearance Solid
Masse moléculaire 223.27
Formule C₁₂H₁₇NO₃
Color White to off-white
SMILES

O=C([C@@H]1O[C@@H]1C(CC/C=C/C/C=C/C)=O)N
Structure Classification
- Antibiotics
- Other Antibiotics
- Antibiotics
- Mammalian Cell Culture
Initial Source
- Microorganisms
Cephalosporium caeruleus
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (12)

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Journal Impact Factor

J Hepatol

ARRB1 inhibits non-alcoholic steatohepatitis progression by promoting GDF15 maturation. [Abstract]

2020 May;72(5):976-989. PMID: 31857195
Cell Metab

Metabolic-Pathway-Based Subtyping of Triple-Negative Breast Cancer Reveals Potential Therapeutic Targets. [Abstract]

2021 Jan 5;33(1):51-64.e9. PMID: 33181091
Cell Res

Signal-induced NLRP3 phase separation initiates inflammasome activation. [Abstract]

2025 Apr 1. PMID: 40164768
Cancer Commun (Lond)

circCAPRIN1 interacts with STAT2 to promote tumor progression and lipid synthesis via upregulating ACC1 expression in colorectal cancer. [Abstract]

2023 Jan;43(1):100-122. PMID: 36328987
Redox Biol

Acetylation of fatty acid synthase regulates microglial lipid droplets accumulation and pro-inflammatory activity following traumatic brain injury. [Abstract]

2025 Dec 15:89:103978. PMID: 41412037

Cerulenin purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Dec 15:89:103978. [Abstract]

Representative immunofluorescence images indicate lipid droplets accumulation in primary microglia ex vivo from FASN wildtype mice or FASN K673R mice, after TBI or not, with or without Cerulenin (5 μg/mL, 24 h) administration (n = 6 mice per group).

Cerulenin purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Dec 15:89:103978. [Abstract]

Representative immunofluorescence and bright field images indicate phagocytosis of primary microglia ex vivo from FASN wildtype mice or FASN K673R mice, after TBI or not, with or without Cerulenin (5 μg/mL, 24 h) administration (n = 6 per group).

Cerulenin purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Dec 15:89:103978. [Abstract]

The percentage of pHRodo Zymosan + microglia in total primary microglia ex vivo from FASN wildtype mice or FASN K673R mice, after TBI or not, with or without Cerulenin (5 μg/mL, 24 h) administration.

Cerulenin purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Dec 15:89:103978. [Abstract]

The mRNA expressions of these cytokines in primary microglial from K673R mice 72h after TBI were significantly reduced, which is similar with the Cerulenin (5 μg/mL, 24 h) inhibition.
Cell Death Dis

MiR-422a regulates cellular metabolism and malignancy by targeting pyruvate dehydrogenase kinase 2 in gastric cancer. [Abstract]

2018 May 2;9(5):505. PMID: 29725130

Cerulenin purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 May 2;9(5):505. [Abstract]

FACS analysis (above) and statistical results (below) of ROS levels after treatment with 2 mg/L Cerulenin or 0.7 mol/L Glycerol for 24 h.
New Phytol

SlWRI5a and SlHY5 co-activate SlFatM-mediated fatty acid biosynthesis during arbuscular mycorrhizal symbiosis in tomato. [Abstract]

2025 Nov 3. PMID: 41185456
Mol Ther Oncolytics

Protein Palmitoylation Regulates Cell Survival by Modulating XBP1 Activity in Glioblastoma Multiforme. [Abstract]

2020 May 22;17:518-530. PMID: 33024813
Microbiol Spectr

Fatty acid synthase may facilitate the trafficking of bovine alpha herpesvirus 1 out of the Golgi apparatus, potentially promoting viral infection. [Abstract]

2025 Nov 6;13(12):e0138825. PMID: 41196069
J Biochem Mol Toxicol

HIF1A/PCDH7 axis mediates fatty acid synthesis and metabolism to inhibit lung adenocarcinoma anoikis. [Abstract]

2024 Nov;38(11):e70001. PMID: 39425457
Cancer Manag Res

Dihydrocapsaicin Secreted by RYK Silenced Bone Marrow-Derived Mesenchymal Stem Cells Triggers Apoptosis of Gastric Cancer Cells. [Abstract]

2026 Mar 21:18:579598. PMID: 41890221
Res Sq

2025 Oct 6.

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (447.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.4789 mL

22.3944 mL

44.7888 mL

5 mM

0.8958 mL

4.4789 mL

8.9578 mL

10 mM

0.4479 mL

2.2394 mL

4.4789 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (11.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (11.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocole

Cell Assay^[2]

Rat aortic endothelial cells (RAECs) are isolated and cultured with minor modifications. Briefly, segments of thoracic aorta are excised from male Wistar rats (150-180 g) and immediately placed in cold PBS containing 100 U/mL Penicillin and 100 mg/mL Streptomycin. The aorta is cut into 1 millimeter wide rings after the periadventitial fat is removed. Following transferred to a T-25 cm² flasks, the rings are cultured in Medium 199 containing 20% fetal bovine serum, 2.5 ng/mL basic fibroblast growth factors, 100U/mL Penicillin and 100 mg/mL Streptomycin. The aorta rings are placed at 37°C in a humidified atmosphere with 5% CO₂ for 72-80 h without movement. All pieces of aorta rings are removed when cells migrated. Its microvascular cytological characteristics are demonstrated by CD31 and vWF staining. In experiments involving PA treatment, M199 medium supplemented with 1% bovine serum albumin is used. All experiments are performed with RAECs up to passage 4. In the experiments with inhibitor, 5 μg/mL Cerulenin (in ethonal), or 5 μM Lovastatin (in DMSO), or 3.3 μg/mL Cerulenin plus 3.3 μM Lovastatin is added in culture media 24 hours prior to PA treatment. The same volume of solvents is added at the same time as control^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[3]

mice^[3]
Cerulenin is given to 6-8 week old male ob/ob mice in RPMI medium containing 20% DMSO intraperitoneally (i.p.). Controls are injected similarly with vehicle alone. The experimental groups (4 mice each) are as follows: A: 60 mg/kg/day Cerulenin, injected daily for 7 days; B: 60 mg/kg every other day for 7 days; C: 30 mg/kg/day for 7 days; D: 30 mg/kg every other day for 7 days; E: vehicle, daily for 7 days; F: 60 mg/kg/day Cerulenin for 2 days; G: 30 mg/kg/day Cerulenin for 2 days; H: vehicle, daily for 2 days; I: control. All animals are sacrificed on the same day under anesthesia. Blood is collected by portal vein puncture. Liver samples are snap-frozen in liquid N₂ and stored at -80°C until analysis, or paraformaldehyde-fixed for histological analysis.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (277 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Bitencourt TA, et al. Trans-chalcone and quercetin down-regulate fatty acid synthase gene expression and reduce ergosterol content in the human pathogenic dermatophyte Trichophyton rubrum. BMC Complement Altern Med. 2013 Sep 17;13:229. [Content Brief]

[2]. Tian D, et al. Overexpression of steroidogenic acute regulatory protein in rat aortic endothelial cells attenuates palmitic acid-induced inflammation and reduction in nitric oxide bioavailability. Cardiovasc Diabetol. 2012 Nov 21;11:144. [Content Brief]

[3]. Cheng G, et al. Cerulenin blockade of fatty acid synthase reverses hepatic steatosis in ob/ob mice. PLoS One. 2013 Sep 27;8(9):e75980. [Content Brief]

[4]. Jeong NY, et al. Fatty acid synthase inhibitor cerulenin inhibits topoisomerase I catalytic activity and augments SN-38-induced apoptosis. Apoptosis. 2013;18(2):226-237. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.4789 mL	22.3944 mL	44.7888 mL	111.9720 mL
	5 mM	0.8958 mL	4.4789 mL	8.9578 mL	22.3944 mL
	10 mM	0.4479 mL	2.2394 mL	4.4789 mL	11.1972 mL
	15 mM	0.2986 mL	1.4930 mL	2.9859 mL	7.4648 mL
	20 mM	0.2239 mL	1.1197 mL	2.2394 mL	5.5986 mL
	25 mM	0.1792 mL	0.8958 mL	1.7916 mL	4.4789 mL
	30 mM	0.1493 mL	0.7465 mL	1.4930 mL	3.7324 mL
	40 mM	0.1120 mL	0.5599 mL	1.1197 mL	2.7993 mL
	50 mM	0.0896 mL	0.4479 mL	0.8958 mL	2.2394 mL
	60 mM	0.0746 mL	0.3732 mL	0.7465 mL	1.8662 mL
	80 mM	0.0560 mL	0.2799 mL	0.5599 mL	1.3997 mL
	100 mM	0.0448 mL	0.2239 mL	0.4479 mL	1.1197 mL

Keywords

Cerulenin

12 Cited Publications

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Cerulenin

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Publications Citing Use of MedChemExpress (MCE) Cerulenin

Voir tous les produits spécifiques à Isoform Antibiotic

Activité biologique

Chemical Information

Publications (12)

Solvant et solubilité

Protocole

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Cerulenin Related Classifications

HY-A0210 Related Classifications