Cat. No.: HY-N3911

Fupenzic acid

Fupenzic acid is a triterpenoid compound that can be found in the fruits of plants in the genus Rubus. Fupenzic acid inhibits LPS (HY-D1056)-induced NF-κB activation and reduces the expression of NO, NOS-2, COX-2, inflammatory cytokines, and chemokines. Fupenzic acid binds to RBMS1, downregulates RBMS1/cGAS/STING signaling, enhances PINK1/Parkin-mediated mitophagy, and alleviates oxidative stress and radiation-induced liver fibrosis. Fupenzic acid exhibits cytotoxic activity against various tumor cells and can inhibit melanin production. Fupenzic acid has inactivation, protective, and replication-inhibitory activities against TMV and CMV. Fupenzic acid can be used in research related to liver fibrosis, malignant glioma, colon cancer, gastric cancer, hepatocellular carcinoma, TMV, and CMV infection.

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CAS No.: 119725-20-1
Formule: C₃₀H₄₄O₅
Masse moléculaire:484.67

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (296 KB)

Fupenzic acid Chemical Structure

CAS No.: 119725-20-1

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

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Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC50	16.31 μM	Cytotoxicity against human HCT116 colon carcinoma cells measured by MTT assay after 48 hrs incubation. Cytotoxicity against human HCT116 colon carcinoma cells measured by MTT assay after 48 hrs incubation.	34923054
BGC-823	IC50	21.94 μM	Cytotoxicity against human BGC823 gastric carcinoma cells measured by MTT assay after 48 hrs incubation. Cytotoxicity against human BGC823 gastric carcinoma cells measured by MTT assay after 48 hrs incubation.	34923054
HepG2	IC50	23.40 μM	Cytotoxicity against human HepG2 hepatocellular carcinoma cells measured by MTT assay after 48 hrs incubation. Cytotoxicity against human HepG2 hepatocellular carcinoma cells measured by MTT assay after 48 hrs incubation.	34923054

In Vitro

Innate ImmunityOxidative StressCytokines & ChemokinesAutophagyMitochondrial FunctionAnti-cancerCarbohydrate MetabolismViral InfectionOthers

Innate Immunity

Oxidative Stress

Fupenzic acid (0-50 µM; 30 min) inhibits LPS (HY-D1056)-induced NO release in mouse peritoneal macrophages, with an IC₅₀ of 16.7 µM^[1].
Fupenzic acid (10-20 µM; 30 min) inhibits LPS-induced NOS-2 and COX-2 protein expression in mouse peritoneal macrophages^[1].

Innate Immunity

Cytokines & Chemokines

Oxidative Stress

Fupenzic acid (10-20 µM; 30 min) downregulates LPS-induced NOS-2, COX-2, IL-6, IL-10, IL-12, IL-18, KC, TNF-α, and IP-10 mRNA expression in mouse peritoneal cells^[1].

Innate Immunity

Fupenzic acid (0-90 min) impairs IκBα/IκBβ degradation and reduces nuclear NF-κB p65 accumulation in LPS-activated mouse peritoneal macrophages^[1].
Fupenzic acid (10-20 μM) downregulates RBMS1/cGAS/STING signaling in TGF-β1-stimulated LX-2 cells^[2].
Fupenzic acid (20 μM) modulates the interaction of RBMS1 with cGAS and STING in LX-2 cells^[2].

Oxidative Stress

Cytokines & Chemokines

Autophagy

Mitochondrial Function

Fupenzic acid (10-20 μM; 24 h) attenuates TGF-β1-induced oxidative stress, inflammation, and mitochondrial dysfunction in LX-2 cells^[2].

Innate Immunity

Cytokines & Chemokines

Fupenzic acid (20 μM; 24 h) inhibits cGAS-STING signaling and fibrosis/inflammation gene expression in LX-2 cells via RBMS1^[2].

Anti-cancer

Fupenzic acid (48 h) shows selective cytotoxicity against HCT116, BGC823, and HepG2 cells, with IC₅₀ values of 16.31 μM, 21.94 μM, and 23.40 μM, respectively^[4].

Carbohydrate Metabolism

Fupenzic acid does not inhibit α-glucosidase^[4].

Viral Infection

Fupenzic acid (100 mg/L) shows 25.11% TMV inactivation, 22.89% CMV inactivation, and 19.36% TMV protection in Nicotiana glutinosa/Chenopodium amaranticolor leaf assays^[5].
Fupenzic acid (100 mg/L) inhibits TMV replication by 22.7640%^[5].

Others

Fupenzic acid (10-20 μM) directly binds RBMS1 in LX-2 lysates and increases RBMS1 thermal stability in CETSA^[2].
Fupenzic acid binds RBMS1 LBD in SPR with a K_D of 0.51 μM^[2].
Fupenzic acid (0.5-160 μM; 24 h) reduces LX-2 cell viability^[2].
Fupenzic acid (10-20 μM; 24 h) inhibits TGF-β1-induced expression of fibrosis markers in LX-2 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

Mouse peritoneal macrophages
Concentration:

0, 10 and 20 μM
Incubation Time:

30 min
Result:

Suppressed LPS-induced NOS-2 and COX-2 protein expression in mouse peritoneal macrophages.

Real Time qPCR^[1]

Cell Line:

Mouse peritoneal cells
Concentration:

0, 10 and 20 μM
Incubation Time:

30 min
Result:

The compound downregulated LPS-induced NOS-2, COX-2, IL-6, IL-10, IL-12, IL-18, KC, TNF-α, and IP-10 mRNA expression in mouse peritoneal cells.

Cell Viability Assay^[2]

Cell Line:

Human hepatic stellate LX-2 cells
Concentration:

0.5, 1, 2.5, 5, 10, 20, 40, 80 and 160 μM
Incubation Time:

24 h
Result:

Inhibited LX-2 cell viability at 40 μM; concentrations of 10 and 20 μM were selected for subsequent experiments.

In Vivo

Innate ImmunityCytokines & ChemokinesOxidative StressMitochondrial FunctionAutophagyAnti-cancerApoptosis

Innate Immunity

Cytokines & Chemokines

Oxidative Stress

Mitochondrial Function

Autophagy

Fupenzic acid (10-20 mg/kg/day; p.o. gavage; once daily for the first month, then once every 3 days from the second to the sixth month; total 6 months) ameliorates RT-induced liver fibrosis in C57BL/6 mice, whereas RBMS1 overexpression attenuates its ameliorative effects on liver injury, fibrosis markers, inflammation, oxidative stress, and mitochondrial dysfunction^[2].

Anti-cancer

Apoptosis

Fupenzic acid exhibits enhanced antitumor efficacy after gelation (FA-gel) in the U118-MG nude mouse model, accompanied by upregulation of caspase-3 and downregulation of Ki67 in tumor tissues^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6 (male, 6-week-old)^[2]
Dosage:

10 mg/kg/day (RT); 20 mg/kg/day (RT); 20 mg/kg/day alone without RT; 20 mg/kg/day (OE-RBMS1 rescue arm)
Administration:

p.o. gavage; daily for first month, then once every 3 days from second to sixth month; 6 months
Result:

Reduced RT-induced serum ALT, AST, and TBIL elevations.
Attenuated the RT-induced increase in liver-to-body weight ratio.
Reduced serum HA, PCIII, LN, and HYP.
Reduced H&E- and Sirius Red-detected fibrosis.
Suppressed COL-IV and Fibronectin immunofluorescence.
Downregulated Col1, Tgfb, Vim, Col4, Fn1, and Timp1 mRNA in liver tissue.
Suppressed RBMS1, cGAS, and STING protein and mRNA expression in fibrotic liver and attenuated RBMS1 immunofluorescence.
Reduced serum IL-6, TNF-α, CXCL1, and CCL1.
Decreased MDA.
Increased SOD.
Modulated Nrf2, Il6, Cxcl1, and Ccl1 mRNA.
Reduced CD86+ macrophage infiltration.
Restored Parkin mRNA.
Reversed RT-induced Parkin/PINK1 decreases and TOMM20 increase.
Preserved mitochondrial integrity by Mito-Tracker staining.
Increased LC3 puncta.
Reduced TOMM20 immunofluorescence.
Transcriptomic comparison of FA-treated versus RT liver identified 2351 differentially expressed genes, with 611 upregulated and 1741 downregulated.
In the RBMS1 overexpression/rescue arm, OE-RBMS1 increased RBMS1 protein and mRNA.
Suppressed RBMS1 protein and mRNA expression and decreased the OE-RBMS1-elevated serum ALT, AST, TBIL, HA, PCIII, and LN.
Downregulated Col1a1, Fn1, Timp1, Il6, and Cxcl1 mRNA.
Upregulated Nrf2 mRNA.
Attenuated H&E/Sirius Red-detected architectural distortion and collagen deposition.
Inhibited cGAS and STING immunofluorescence.
Enhanced Parkin immunofluorescence.

Related Protocols :

Chemical Information

CAS No. 119725-20-1
Masse moléculaire 484.67
Formule C₃₀H₄₄O₅
SMILES

OC([C@]12[C@]([C@](O)([C@H](C)CC2)C)([H])C3=CC[C@@]([C@@]4([C@@](C(C)(C(C(O)=C4)=O)C)([H])CC5)C)([H])[C@]5(C)[C@]3(C)CC1)=O
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (296 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Boukerouis D, et al. Exploring the anti-inflammatory activity of fupenzic acid using network pharmacology and experimental validation. Scientific reports. 2025 Apr 24;15(1):14294.

[2]. Guo X, et al. Fupenzic acid ameliorates radiation-induced liver fibrosis by regulating RBMS1 to inhibit the cGAS-STING pathway and restore mitochondrial function. Phytomedicine : international journal of phytotherapy and phytopharmacology. 2026 Sep;159:158399. [Content Brief]

[3]. Sun Z, et al. A novel nonreversible heat-induced low-molecular-weight gel based on naturally-occurring self-assembled fupenzic acid for tumor therapy. Colloids and surfaces. B, Biointerfaces. 2023 Aug;228:113392.

[4]. Zhang J, et al. The triterpenoids and sesquiterpenoids from the plant of Agrimonia pilosa. Fitoterapia. 2022 Mar;157:105104. [Content Brief]

[5]. Zhang X, et al. Antiphytoviral toxins of Actinidia chinensis root bark (ACRB) extract: laboratory and semi‐field trials. Pest management science. 2018 Jul;74(7):1630-6.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Fupenzic acid

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