Cat. No.: HY-N1724A

Concanamycin A (purity≥70%)

(Synonyms: Antibiotic X 4357B (purity≥70%); Folimycin (purity≥70%); X 4357B (purity≥70%))

28 Cited Publications

Customer Review

Based on 28 publication(s) in Google Scholar

Concanamycin A (purity≥70%) (Antibiotic X 4357B) is a macrolide antibiotic, a vacuolar type H⁺-ATPase (V-ATPase) inhibitor. Concanamycin A (purity≥70%) is also an inhibitor of lysosomal acidification, can be used to T cell-mediated inflammation research^-.

For research use only. We do not sell to patients.

CAS No.: 80890-47-7
Formula: C₄₆H₇₅NO₁₄
Molecular Weight:866.09

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Concanamycin A (purity≥70%) Chemical Structure

CAS No.: 80890-47-7

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (28)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Macrolide

All Antibiotic Isoforms :

In Vitro

Concanamycin A (purity≥70%) can come from S. diastatochromogenes, as a microbial metabolite with immunomodulatory activity^[1].
Concanamycin A (purity≥70%) (100 nM; 0-20 h) results DNA fragmentation in CD4⁺ and selectively induces CD8⁺ T cells rapid cell death between normal and the immunized mice source, while CD8⁺ population in mice immunized is more sensitive^[2].
Concanamycin A (purity≥70%) (3-50 nM; 16 h) inhibits LPS-induced NO production in elicited peritoneal macrophages, but (25 nM; 7 h) doesn’t inhibit LPS-induced TNF-α production^[3].
Concanamycin A (purity≥70%) (0.01 nM-1 nM) inhibits the acidification of rat liver lysosomes (IC₅₀ =0.061 nM), and inhibits oleate incorporation into cholesteryl ester (IC₅₀ =14 nM)^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

CD8⁺ cytotoxic T lymphocytes (CTLs)
Concentration:

100 nM
Incubation Time:

0, 4, 8, 12, 16, 20 hours
Result:

Induced rapid cell death instead of apoptosis at 20 h, without observed condensed nuclei.

In Vivo

Concanamycin A (purity≥70%) (15 mg/kg; i.v.; 0, 10 or 24 h prior to sacrifice) induces T cell-mediated hepatitis in mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Wild type mice^[5]
Dosage:

15 mg/kg
Administration:

Intravenous injection 0, 10 or 24 h prior to sacrifice
Result:

Resulted significant liver injury as demonstrated by serum transaminase levels, inflammatory cell infiltrate, hepatocyte apoptosis, and expression of several cytokines including interleukin 4 (IL4) and interferon gamma (IFNγ) at 10 h and 24 h following administration.

Chemical Information

CAS No. 80890-47-7
Molecular Weight 866.09
Formula C₄₆H₇₅NO₁₄
SMILES

C[C@H]([C@@](C[C@H]1O[C@@](O[C@H](C)[C@H]2OC(N)=O)([H])C[C@H]2O)(O[C@H](/C=C/C)[C@H]1C)O)[C@H](O)[C@@H]([C@](OC(/C(OC)=C/C(C)=C/[C@H]([C@@H](O)[C@H]3CC)C)=O)([H])[C@H](/C=C/C=C(C[C@H]([C@@H]3O)C)/C)OC)C
Synonyms

Antibiotic X 4357B (purity≥70%); Folimycin (purity≥70%); X 4357B (purity≥70%)
Structure Classification
- Antibiotics
Initial Source
- Microorganisms
Streptomyces diastatochromogenes S-45
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (28)

All Cell Proliferation/Viability AssayCell Imaging/StainingWB

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Nat Nanotechnol

Targeted protein degradation via cellular trafficking of nanoparticles. [Abstract]

2025 Feb;20(2):296-302. PMID: 39468359

Concanamycin A (purity≥70%) purchased from MedChemExpress. Usage Cited in: Nat Nanotechnol. 2025 Feb;20(2):296-302. [Abstract]

EGFR protein degradation evaluation in MDA-MB-231 cells after treatment with 500 nM NTZ-NPs together with CQ, Baf and Concanamycin A (ConA, 10 nM) for 24 h. Note that for the inhibitors, solvent dimethylsulfoxide (DMSO) represents the control group.
Cell Res

A single small molecule-based human embryo model reveals V-ATPase requirement in mammalian blastocyst cavitation. [Abstract]

2026 Jul;36(7):475-498. PMID: 41936702
Mol Cell

Endocytosis is essential for cysteine-deprivation-induced ferroptosis. [Abstract]

2025 Sep 4;85(17):3333-3342.e4. PMID: 40858109

Concanamycin A (purity≥70%) purchased from MedChemExpress. Usage Cited in: Mol Cell. 2025 Sep 4;85(17):3333-3342.e4. [Abstract]

Cell death analysis of GPX4 KO HT1080 cells treated with 50 μM deferoxamine (DFO), 2 μM Lip-1, 100 nM BafA1, 100 nM Concanamycin A (ConA), in the absence or presence of 2 μM Trolox as indicated.

Concanamycin A (purity≥70%) purchased from MedChemExpress. Usage Cited in: Mol Cell. 2025 Sep 4;85(17):3333-3342.e4. [Abstract]

Concanamycin A (ConA; 100 nM; 6 h, a lysosomal inhibitor) prevented lipid peroxidation induced by cysteine deficiency in HT1080 cells.
Nat Commun

ATG8ylation-mediated tonoplast invagination mitigates vacuole damage. [Abstract]

2025 Jul 18;16(1):6621. PMID: 40681515

Concanamycin A (purity≥70%) purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 18;16(1):6621. [Abstract]

Pretreatment of Concanamycin A (ConcA, 1 μM; 10 min) inhibited the translocation of GFP-ATG8a to the tonoplast. ConcA was added 10 min prior to monensin treatment. Scale bar, 20 μm.

Concanamycin A (purity≥70%) purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 18;16(1):6621. [Abstract]

Concanamycin A (ConcA, 1 μM; 10 min) resulted in a significant reduction in the lipidation form of ATG8 following Monensin (20 μM; 1 h) treatment in mammalian cells.
Autophagy

The Valsa mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple. [Abstract]

2023 Jun;19(6):1745-1763. PMID: 36449354
Adv Sci (Weinh)

ERAD Component MoHrd3 Facilitates Pathogenicity and Establishes a Direct Regulation on Autophagy in Magnaporthe Oryzae. [Abstract]

2026 Apr;13(24):e20627. PMID: 41725610
Adv Sci (Weinh)

OTUD6A in Airway Epithelial Cells Exacerbates Allergic Asthma by Promoting Airway Inflammation and Airway Remodeling Through Deubiquitination of hResistin/mRELMα. [Abstract]

2026 Jan 20:e16355. PMID: 41560298
Adv Sci (Weinh)

CCDC41 Drives Oocyte Meiotic Progression by Promoting Rab11a/Rab7-Positive Vesicle Fusion with Target Membranes. [Abstract]

2025 Dec 2:e04665. PMID: 41331237
Nat Plants

An apoplastic fungal effector disrupts N-glycosylation of ZmLecRK1, inducing its degradation to suppress disease resistance in maize. [Abstract]

2025 Sep 12. PMID: 40940425
Biomaterials

Biomimetic engineering peptidyl-based vehicles for efficient mRNA delivery. [Abstract]

2025 Dec 18:329:123925. PMID: 41453350
Sci Adv

A noncanonical function of SKP1 regulates the switch between autophagy and unconventional secretion. [Abstract]

2023 Oct 13;9(41):eadh1134. PMID: 37831778
Cell Death Dis

Plk1 promotes renal tubulointerstitial fibrosis by targeting autophagy/lysosome axis. [Abstract]

2023 Aug 29;14(8):571. PMID: 37640723
Dev Cell

Ferroptosis induces heterogeneous death profiles that are controlled by lysosome rupture. [Abstract]

2026 Feb 17:S1534-5807(26)00037-7. PMID: 41709461
Dev Cell

PIF4 controls autophagy during hypocotyl response to elevated ambient temperatures via ArpC5 in Arabidopsis. [Abstract]

2025 Jul 9:S1534-5807(25)00402-2. PMID: 40669463
Free Radic Biol Med

Glucose deprivation-restoration induces labile iron overload and ferroptosis in renal tubules through V-ATPase-mTOR axis-mediated ferritinophagy and iron release by TPC2. [Abstract]

2025 Aug 16:236:204-219. PMID: 40379157
Biomed Pharmacother

Lysosomal sequestration of weak base drugs, lysosomal biogenesis, and cell cycle alteration. [Abstract]

2022 Sep:153:113328. PMID: 35785701
Cell Rep

TCF25 serves as a nutrient sensor to orchestrate metabolic adaptation and cell death by enhancing lysosomal acidification under glucose starvation. [Abstract]

2025 Aug 21;44(9):116186. PMID: 40844875
Plant Physiol

Ubiquitylation of PHYTOSULFOKINE RECEPTOR 1 modulates the defense response in tomato. [Abstract]

2023 Jul 3;192(3):2507-2522. PMID: 36946197
J Cell Biol

ATG16L1 controls mammalian vacuolar proton ATPase. [Abstract]

2025 Oct 6;224(10):e202503166. PMID: 40923996
Mol Med

Extracellular CIRP induces CD4CD8αα intraepithelial lymphocyte cytotoxicity in sepsis. [Abstract]

2024 Feb 1;30(1):17. PMID: 38302880
Commun Biol

Measuring pH in insulin secretory granules by phasor-based fluorescence lifetime imaging of a genetically encoded sensor. [Abstract]

2025 Feb 25;8(1):304. PMID: 40000885
J Virol

The role of lysosomes as intermediates in betacoronavirus PHEV egress from nerve cells. [Abstract]

2023 Dec 21;97(12):e0133823. PMID: 38009916
J Cancer

Upregulation of V-ATPase by STAT3 Activation Promotes Anoikis Resistance and Tumor Metastasis. [Abstract]

2021 Jun 11;12(16):4819-4829. PMID: 34234852
Seoul National University

2025.
bioRxiv

2025 Sep 30.
Res Sq

2024 Oct 17.
bioRxiv

2024 Apr 26.
bioRxiv

2023 Mar 24.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Vaněk, Z., et al. Immunomodulators isolated from microorganisms. Folia Microbiol. 1991. 36:99–111.

[2]. Togashi K, et al. Concanamycin A, a vacuolar type H(+)-ATPase inhibitor, induces cell death in activated CD8(+) CTL. Cytotechnology. 1997 Nov;25(1-3):127-35. [Content Brief]

[3]. Eswarappa SM, et al. Folimycin (concanamycin A) inhibits LPS-induced nitric oxide production and reduces surface localization of TLR4 in murine macrophages. Innate Immun. 2008 Feb;14(1):13-24. [Content Brief]

[4]. Hines IN, et al. Impaired T cell-mediated hepatitis in peroxisome proliferator activated receptor alpha (PPARα)-deficient mice. Biol Res. 2018 Feb 15;51(1):5. [Content Brief]

[5]. Woo JT, et al. Isolation, characterization and biological activities of concanamycins as inhibitors of lysosomal acidification. J Antibiot (Tokyo). 1992 Jul;45(7):1108-16. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Concanamycin A (purity≥70%)

28 Cited Publications

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Concanamycin A (purity≥70%)

Publications Citing Use of MedChemExpress (MCE) Concanamycin A (purity≥70%)

All Antibiotic Isoforms

Biological Activity

Chemical Information

Publications (28)

Purity & Documentation

References

Calculators

Help & FAQs

Concanamycin A (purity≥70%) Related Classifications

HY-N1724A Related Classifications