Cat. No.: HY-W020050

Cystamine dihydrochloride

7 Cited Publications

Customer Review

Based on 7 publication(s) in Google Scholar

Cystamine (dihydrochloride) is the disulfide form of the free thiol, cysteamine. Cystamine is an orally active transglutaminase (Tgase) inhibitor. Cystamine also has inhibition activity for caspase-3 with an IC₅₀ value of 23.6 μM. Cystamine can be used for the research of severals diseases including Huntington's disease (HD) .

For research use only. We do not sell to patients.

Purity: 99.92%
CAS No.: 56-17-7
Formula: C₄H₁₄Cl₂N₂S₂
Molecular Weight:225.20

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Cystamine dihydrochloride Chemical Structure

CAS No.: 56-17-7

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (7)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

Caspase 3

23.6 μM (IC₅₀)

All Caspase Isoforms :

In Vitro

Cystamine (dihydrochloride) has inhibition activity for caspase-3 with an IC₅₀ value of 23.6 μM^[1].
Cystamine (0-500 μM; 0-16 h) inhibits recombinant active caspase-3 in a concentration-dependent manner^[1].
Cystamine (250 μM; 10 h) robustly increases the levels of glutathione^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

Human neuroblastoma SH-SY5Y cells
Concentration:

250, 500 μM
Incubation Time:

0-16 h
Result:

Inhibited the MG132-mediated activation of caspase-3.
Inhibited the H2O2-mediated activation of caspase-3.
Inhibited caspase-3 activity in a tTG-independent manner.

In Vivo

Cystamine (dihydrochloride) (oral, i.p.; 112, 225 mg/kg) reduces Tgase activity and GGEL levels, lessens the behavioral and neuropathological severity, and extends survival in R6/2 transgenic HD mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

R6/2 transgenic HD mice^[2]
Dosage:

112, 225 mg/kg
Administration:

Intraperitoneal or oral, daily
Result:

Significantly extended survival, improved body weight and motor performance, delayed the neuropathological sequela and significantly altered the levels of Tgase activity and N(Sigma)-(gamma-L-glutamyl)-L-lysine (GGEL) levels.

Chemical Information

CAS No. 56-17-7
Appearance Solid
Molecular Weight 225.20
Formula C₄H₁₄Cl₂N₂S₂
Color White to off-white
SMILES

NCCSSCCN.[H]Cl.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (7)

All WBIFHistological Imaging/StainingIHCELISA

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Journal Impact Factor

Adv Sci (Weinh)

TGM2 Aggravates Acute Pancreatitis by Impairing Macrophage Efferocytosis Through Inhibition of the STAT6-GAS6 Axis. [Abstract]

2026 Apr;13(24):e20739. PMID: 41703992

Cystamine dihydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Apr;13(24):e20739. [Abstract]

Cystamine (225 mg/kg; i.p.; once daily for 7 d) effectively suppressed TGM2 expression in pancreatic tissues of AP model mice.

Cystamine dihydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Apr;13(24):e20739. [Abstract]

Cystamine (225 mg/kg; i.p.; once daily for 7 d) effectively inhibited TGM2 expression in pancreatic tissues of AP mice.

Cystamine dihydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Apr;13(24):e20739. [Abstract]

Cystamine (225 mg/kg; i.p.; once daily for 7 d) pretreatment alleviates pancreatic damage in AP mice.

Cystamine dihydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Apr;13(24):e20739. [Abstract]

Cystamine (225 mg/kg; i.p.; once daily for 7 d) pretreatment reduced IL-1β, IL-6, and TNF-α expression in pancreatic tissues of AP mice.

Cystamine dihydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Apr;13(24):e20739. [Abstract]

Cystamine (225 mg/kg; i.p.; once daily for 7 d) decreased serum amylase activity and levels of IL-1β, IL-6, and TNF-α in AP mice.
Small

Homocysteine-Responsive Covalent Organic Frameworks as Signaling Scaffolds: Modulating Transsulfuration for Depression Treatment. [Abstract]

2025 Jun;21(23):e2501944. PMID: 40245113
Phytomedicine

β,β-Dimethylacrylshikonin suppresses hepatocellular carcinoma progression through mitochondrial stress and TGM2-associated mitophagy impairment. [Abstract]

2026 Jul 25:157:158322. PMID: 42176510
ACS Environ Au

Machine Learning-Assisted Recognition of Environmental Sulfur-Containing Chemicals in Nontargeted Mass Spectrometry Analysis of Inadequate Mass Resolution. [Abstract]

2025 Aug 5;5(6):573-582. PMID: 41277996
Anal Chem

Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]

2025 Jun 3;97(21):11099-11109. PMID: 40401576
CNS Neurosci Ther

Inhibition of Transglutaminase 2 Preserves Blood-Brain Barrier Integrity and Improves Neurological Outcomes After Experimental Traumatic Brain Injury in Mice. [Abstract]

2026 Apr;32(4):e70887. PMID: 42002982
Discov Oncol

Transglutaminase 2 nuclear localization enhances glioblastoma radiation resistance. [Abstract]

2025 May 30;16(1):952. PMID: 40445445

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (444.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (444.05 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.4405 mL

22.2025 mL

44.4050 mL

5 mM

0.8881 mL

4.4405 mL

8.8810 mL

10 mM

0.4440 mL

2.2202 mL

4.4405 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (11.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (11.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (11.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (453 KB)
- English - EN (453 KB)
- Français - FR (453 KB)
- Deutsch - DE (453 KB)
- Norwegian - NO (453 KB)
- Español - ES (453 KB)
- Swedish - SV (453 KB)
- Italian - IT (453 KB)
- Korean - KR (453 KB)
- Portuguese - PT (453 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Mathieu Lesort, et al. Cystamine inhibits caspase activity. Implications for the treatment of polyglutamine disorders. J Biol Chem. 2003 Feb 7;278(6):3825-30. [Content Brief]

[2]. Alpaslan Dedeoglu, et al. Therapeutic effects of cystamine in a murine model of Huntington's disease. J Neurosci. 2002 Oct 15;22(20):8942-50. [Content Brief]

[3]. Thomas M Jeitner, et al. Cystamine and cysteamine as inhibitors of transglutaminase activity in vivo. Biosci Rep. 2018 Sep 5;38(5):BSR20180691. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	4.4405 mL	22.2025 mL	44.4050 mL	111.0124 mL
	5 mM	0.8881 mL	4.4405 mL	8.8810 mL	22.2025 mL
	10 mM	0.4440 mL	2.2202 mL	4.4405 mL	11.1012 mL
	15 mM	0.2960 mL	1.4802 mL	2.9603 mL	7.4008 mL
	20 mM	0.2220 mL	1.1101 mL	2.2202 mL	5.5506 mL
	25 mM	0.1776 mL	0.8881 mL	1.7762 mL	4.4405 mL
	30 mM	0.1480 mL	0.7401 mL	1.4802 mL	3.7004 mL
	40 mM	0.1110 mL	0.5551 mL	1.1101 mL	2.7753 mL
	50 mM	0.0888 mL	0.4440 mL	0.8881 mL	2.2202 mL
	60 mM	0.0740 mL	0.3700 mL	0.7401 mL	1.8502 mL
	80 mM	0.0555 mL	0.2775 mL	0.5551 mL	1.3877 mL
	100 mM	0.0444 mL	0.2220 mL	0.4440 mL	1.1101 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Cystamine dihydrochloride

7 Cited Publications

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Cystamine dihydrochloride

Publications Citing Use of MedChemExpress (MCE) Cystamine dihydrochloride

All Caspase Isoforms

Biological Activity

Chemical Information

Publications (7)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Cystamine dihydrochloride Related Classifications

HY-W020050 Related Classifications