2. Apoptosis Metabolic Enzyme/Protease
  3. Caspase Glutaminase Apoptosis
  4. Cystamine

Cystamine is the disulfide form of the free thiol, cysteamine. Cystamine is an orally active transglutaminase (Tgase) inhibitor. Cystamine also has inhibition activity for caspase-3 with an IC50 value of 23.6 μM. Cystamine can be used for the research of severals diseases including Huntington's disease (HD) .

For research use only. We do not sell to patients.

Cystamine Chemical Structure

Cystamine Chemical Structure

CAS No. : 51-85-4

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Based on 1 publication(s) in Google Scholar

Other Forms of Cystamine:

Cystamine Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Cystamine is the disulfide form of the free thiol, cysteamine. Cystamine is an orally active transglutaminase (Tgase) inhibitor. Cystamine also has inhibition activity for caspase-3 with an IC50 value of 23.6 μM. Cystamine can be used for the research of severals diseases including Huntington's disease (HD) [1][2][3].

IC50 & Target[1]

Caspase 3

23.6 μM (IC50)

In Vitro

Cystamine has inhibition activity for caspase-3 with an IC50 value of 23.6 μM[1].
Cystamine (0-500 μM; 0-16 h) inhibits recombinant active caspase-3 in a concentration-dependent manner[1].
Cystamine (250 μM; 10 h) robustly increases the levels of glutathione[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cystamine Related Antibodies

Western Blot Analysis[1]

Cell Line: uman neuroblastoma SH-SY5Y cells
Concentration: 250, 500 μM
Incubation Time: 0-16 h
Result: Inhibited the MG132-mediated activation of caspase-3.
Inhibited the H2O2-mediated activation of caspase-3.
Inhibited caspase-3 activity in a tTG-independent manner.
In Vivo

Cystamine (oral, i.p.; 112, 225 mg/kg) reduces Tgase activity and GGEL levels, lessens the behavioral and neuropathological severity, and extends survival in R6/2 transgenic HD mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: R6/2 transgenic HD mice[2]
Dosage: 112, 225 mg/kg
Administration: Intraperitoneal or oral, daily
Result: Significantly extended survival, improved body weight and motor performance, delayed the neuropathological sequela and significantly altered the levels of Tgase activity and N(Sigma)-(gamma-L-glutamyl)-L-lysine (GGEL) levels.
Molecular Weight

152.28

Formula

C4H12N2S2
CAS No.
Appearance

Liquid

Color

Light yellow to brown

SMILES

NCCSSCCN
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (656.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 100 mg/mL (656.69 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.5669 mL 32.8343 mL 65.6685 mL
5 mM 1.3134 mL 6.5669 mL 13.1337 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (16.42 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (16.42 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.79%

COA (240 KB) HNMR (240 KB) LCMS (258 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 6.5669 mL 32.8343 mL 65.6685 mL 164.1713 mL
5 mM 1.3134 mL 6.5669 mL 13.1337 mL 32.8343 mL
10 mM 0.6567 mL 3.2834 mL 6.5669 mL 16.4171 mL
15 mM 0.4378 mL 2.1890 mL 4.3779 mL 10.9448 mL
20 mM 0.3283 mL 1.6417 mL 3.2834 mL 8.2086 mL
25 mM 0.2627 mL 1.3134 mL 2.6267 mL 6.5669 mL
30 mM 0.2189 mL 1.0945 mL 2.1890 mL 5.4724 mL
40 mM 0.1642 mL 0.8209 mL 1.6417 mL 4.1043 mL
50 mM 0.1313 mL 0.6567 mL 1.3134 mL 3.2834 mL
60 mM 0.1094 mL 0.5472 mL 1.0945 mL 2.7362 mL
80 mM 0.0821 mL 0.4104 mL 0.8209 mL 2.0521 mL
100 mM 0.0657 mL 0.3283 mL 0.6567 mL 1.6417 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Cystamine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cystamine51-85-4CaspaseGlutaminaseApoptosisoralHuntington's disease (HD)transglutaminase (Tgase)disulfidecaspase-3cancerInhibitorinhibitorinhibit

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