Art. -Nr.: HY-17540

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HBX 19818

18 Cited Publications

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Based on 18 publication(s) in Google Scholar

HBX 19818 is a specific inhibitor of ubiquitin-specific protease 7 (USP7), with an IC₅₀ of 28.1 μM.

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Reinheit: 99.25%
CAS. Nr.: 1426944-49-1
Formel: C₂₅H₂₈ClN₃O
Molecular Weight:421.96

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

HBX 19818 Chemical Structure

CAS. Nr.: 1426944-49-1

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (18)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

HBX 19818 is a specific inhibitor of ubiquitin-specific protease 7 (USP7), with an IC₅₀ of 28.1 μM.

IC₅₀ & Target

IC50: 28.1 μM (USP7)^[1]

Alle Deubiquitinase Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	2 μM Compound: HBX19818; Fig 10, Cpd 1	Anticancer activity against human HCT-116 cells assessed as cell growth inhibition Anticancer activity against human HCT-116 cells assessed as cell growth inhibition	[PMID: 32092586]

In Vitro

HBX 19818 is an inhibitor of USP7, with an IC₅₀ of 28.1 μM. HBX 19818 shows no effects on USP8, USP5, USP10, CYLD, UCH-L1, UCH-L3 or on SENP1, a SUMO protease, with IC₅₀s of > 200 μM. HBX 19818 selectively inhibits USP7 with IC₅₀ of ～6 μM in in human cancer cells. In addition, HBX 19818 (1.5, 4, 12, 36, or 100 μM) inhibits USP7 deubiquitination of polyubiquitinated p53. HBX 19818 (30 μM) also causes significantly higher levels of Mdm2 polyubiquitinated forms in USP7-overproducing HEK293 cells than those in DMSO-treated control cells. HBX 19818 inhibits HCT116 proliferation in a dose-dependent manner, with an IC₅₀ of ～2 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 1426944-49-1
Appearance Solid
Molecular Weight 421.96
Formel C₂₅H₂₈ClN₃O
Color Light yellow to yellow
SMILES

CN(CCCNC(C1=CC=C(N=C(CCCC2)C2=C3Cl)C3=C1)=O)CC4=CC=CC=C4
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (18)

All WB

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Journal Impact Factor

Cell Metab

Deubiquitinases Maintain Protein Homeostasis and Survival of Cancer Cells upon Glutathione Depletion. [Abstract]

2019 May 7;29(5):1166-1181.e6. PMID: 30799286
Nat Commun

Usp7 regulates Hippo pathway through deubiquitinating the transcriptional coactivator Yorkie. [Abstract]

2019 Jan 24;10(1):411. PMID: 30679505
Nat Chem Biol

Inhibition of USP10 induces degradation of oncogenic FLT3. [Abstract]

2017 Dec;13(12):1207-1215. PMID: 28967922

HBX 19818 purchased from MedChemExpress. Usage Cited in: Nat Chem Biol. 2017 Dec;13(12):1207-1215. [Abstract]

The effects of HBX19818 on FLT 3 expression in FLT 3-ITD-expressing Ba/F3 cells after 22 h of treatment. The effects of P22077 on FLT 3 levels in FLT 3-ITD-expressing Ba/F3 cells after 22 h of treatment.
Theranostics

USP7 targeting modulates anti-tumor immune response by reprogramming Tumor-associated Macrophages in Lung Cancer. [Abstract]

2020 Jul 23;10(20):9332-9347. PMID: 32802195
Cell Death Differ

Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma. [Abstract]

2023 Feb;30(2):527-543. PMID: 36526897
Nat Struct Mol Biol

USP7 is a SUMO deubiquitinase essential for DNA replication. [Abstract]

2016 Apr;23(4):270-7. PMID: 26950370
EMBO J

USP7 limits CDK1 activity throughout the cell cycle. [Abstract]

2021 Jun 1;40(11):e99692. PMID: 33856059
Br J Cancer

WD repeat-containing protein 1 maintains β-Catenin activity to promote pancreatic cancer aggressiveness. [Abstract]

2020 Sep;123(6):1012-1023. PMID: 32601462
Br J Cancer

Inhibition of the deubiquitinase USP10 induces degradation of SYK. [Abstract]

2020 Apr;122(8):1175-1184. PMID: 32015510
Cell Rep

IGF2BP2 stabilized by USP7 promotes cancer-associated fibroblast activation and attenuates gemcitabine sensitivity in PDAC. [Abstract]

2025 Nov 25;44(11):116476. PMID: 41201091
Cell Rep

Oxidative stress reprograms the transcriptional coactivator Yki to suppress cell proliferation. [Abstract]

2024 Aug 5;43(8):114584. PMID: 39106181
Cell Rep

SENP3 and USP7 regulate Polycomb-rixosome interactions and silencing functions. [Abstract]

2023 Apr 3;42(4):112339. PMID: 37014752
Int J Oncol

circRPS19 affects HK2‑mediated aerobic glycolysis and cell viability via the miR‑125a‑5p/USP7 pathway in gastric cancer. [Abstract]

2023 Aug;63(2):98. PMID: 37449524
J Biol Chem

Ubiquitin-specific protease 7 promotes the growth and oncogenic potential of acute myeloid leukemia cells through the deubiquitination and upregulation of LRRK2. [Abstract]

2025 Sep 2;301(10):110675. PMID: 40907896
Anim Cells Syst

Blocking SLC7A11 attenuates the proliferation of esophageal squamous cell carcinoma cells. [Abstract]

2024 May 11;28(1):237-250. PMID: 38741950
Arch Pharm (Weinheim)

Synthesis and structure-activity relationships of USP48 deubiquitinylase inhibitors. [Abstract]

2023 Jul;356(7):e2200661. PMID: 37196427
Mol Cell Biol

USP7 cooperates with SCML2 to regulate the activity of PRC1. [Abstract]

2015 Apr 1;35(7):1157-68. PMID: 25605328

HBX 19818 purchased from MedChemExpress. Usage Cited in: Mol Cell Biol. 2015 Apr 1;35(7):1157-68. [Abstract]

Western blot analysis of the chromatin fraction of HCT116 cells after treatment with the USP7 inhibitor HBX19818 for 4 or 8 h at 50 μM. Expression levels of USP7, RING1B, BMI1, and Ub-H2A are analyzed.
Dev Growth Differ

Ubiquitin-specific protease 7 (USP7) is essential for endometrial stromal cell decidualization in mice. [Abstract]

2019 Feb;61(2):176-185. PMID: 30628051

Lösungsmittel & Löslichkeit

In Vitro:

1M HCl : 100 mg/mL (236.99 mM; ultrasonic and adjust pH to 1 with HCl)

DMSO : 20 mg/mL (47.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3699 mL

11.8495 mL

23.6989 mL

5 mM

0.4740 mL

2.3699 mL

4.7398 mL

10 mM

0.2370 mL

1.1849 mL

2.3699 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2 mg/mL (4.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2 mg/mL (4.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protokoll

Kinase Assay^[1]

The ability of HBX 19818 and HBX 28,258 to inhibit a panel of deubiquitinating enzymes, including UCH-L3 (13 pM), USP7 (100 pM), USP8 (1.36 nM), UCH-L1 (2.5 nM), USP5 (10 nM), USP20 (10 nM), and USP2 (500 pM), is tested using the UbAMC substrate (300 nM). The potential effects of HBX 19818 and HBX 28,258 are also tested on the enzymatic activities of SENP1 (80 pM), cathepsin-B (100 pM), and caspase-3 (100 pM) using the SUMO1-AMC (750 nM), ZRR-AMC (3 μM), and DEVD-AMC (250 nM) substrates, respectively. All enzymes are tested in USP7 reaction buffer (50 mM Tris-HCl [pH 7.6], 0.5 mM EDTA, 5 mM DTT, 0.01% Triton X-100, and 0.05 mg/mL serum albumin), except for two enzymes, USP8 (same buffer but pH 8.8) and caspase-3 (100 mM HEPES [pH 7.5], 10% sucrose, and 0.1% CHAPS). All enzymes are pre-incubated with DMSO or compounds (including HBX 19818) for 30 min at room temperature, and the enzymatic reaction is initiated by adding the substrate of interest. The reaction mixture is incubated at room temperature for 1 hr, and the reaction is stopped by adding acetic acid (100 mM). The reactions are monitored using the PHERAstar^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay^[1]

HCT116 cell proliferation is evaluated by incubating HCT116 cells for 30 min in culture medium containing 10 µM 5- bromo-2-deoxyuridine (BrdU), which is incorporated into the DNA of proliferating cells. Cells are then harvested by trypsin treatment, collected by centrifugation, and the pellet is resuspended and incubated in 70% ethanol for 30 min at 4°C. After centrifugation and supernatant removal, DNA is denaturated by incubating it in 2 N HCl for 30 min at room temperature. The percentage of BrdU-containing cells is then determined by flow cytometry, making it possible to quantify proliferating cells. Cell cycle is evaluated after treatment with HBX 19818 for 24 hr, followed by fixing detached cells and trypsinized cells in 70% ethanol for 30 minutes at 4°C. Cells are then incubated in PBS supplemented with 1% BSA, 0.5% Tween 20, 50 µg/mL RNase A and 50 µg/mL propidiumiodide for 30 minutes at 37°C. Samples are analyzed on a FACSort fluorocytometer. The percentage of cells in the different phases of the cell cycle is calculated using Multicycle software^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Reverdy C, et al. Discovery of specific inhibitors of human USP7/HAUSP deubiquitinating enzyme. Chem Biol. 2012 Apr 20;19(4):467-77. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / 1M HCl	1 mM	2.3699 mL	11.8495 mL	23.6989 mL	59.2473 mL
	5 mM	0.4740 mL	2.3699 mL	4.7398 mL	11.8495 mL
	10 mM	0.2370 mL	1.1849 mL	2.3699 mL	5.9247 mL
	15 mM	0.1580 mL	0.7900 mL	1.5799 mL	3.9498 mL
	20 mM	0.1185 mL	0.5925 mL	1.1849 mL	2.9624 mL
	25 mM	0.0948 mL	0.4740 mL	0.9480 mL	2.3699 mL
	30 mM	0.0790 mL	0.3950 mL	0.7900 mL	1.9749 mL
	40 mM	0.0592 mL	0.2962 mL	0.5925 mL	1.4812 mL
1M HCl	50 mM	0.0474 mL	0.2370 mL	0.4740 mL	1.1849 mL
	60 mM	0.0395 mL	0.1975 mL	0.3950 mL	0.9875 mL
	80 mM	0.0296 mL	0.1481 mL	0.2962 mL	0.7406 mL
	100 mM	0.0237 mL	0.1185 mL	0.2370 mL	0.5925 mL

Keywords

HBX 19818

18 Cited Publications

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HBX 19818

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Covalent Screening Library

Ubiquitination Compound Library

Metabolic Enzyme Compound Library

Protease Inhibitor Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) HBX 19818

Alle Deubiquitinase Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (18)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

HBX 19818

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Covalent Screening Library

Ubiquitination Compound Library

Metabolic Enzyme Compound Library

Protease Inhibitor Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) HBX 19818

Alle Deubiquitinase Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (18)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

HBX 19818 Related Classifications

HY-17540 Related Classifications