Cat. No.: HY-121263

Dicyclanil

Name: Dicyclanil
Brand: MedChemExpress
SKU: HY-121263
Price: 55.00 USD
Availability: InStock
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Dicyclanil is an orally active insect growth regulator. Dicyclanil upregulates hepatic OGG1 DNA repair gene expression and Cyp1a1 expression, and generates reactive oxygen species (ROS) during microsomal oxidation. Dicyclanil induces oxidative stress, oxidative DNA damage, and female-specific hepatocellular proliferation. Dicyclanil can be used in research on hepatocellular carcinoma and myiasis.

For research use only. We do not sell to patients.

Purity : 99.18%
CAS No.: 112636-83-6
Formula: C₈H₁₀N₆
Molecular Weight:190.21

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Dicyclanil Chemical Structure

CAS No.: 112636-83-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

HY-121263R
Dicyclanil (Standard)

Reference Standard

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]^[2]^[3]^[4]^[5]^[6]^[7]^[8]^[9]

CYP1A1

OGG1

All DNA/RNA Synthesis Isoforms :

All Cytochrome P450 Isoforms :

In Vitro

Oxidative StressParasitic Infection

Oxidative Stress

Dicyclanil (DC) (0.01-3.0 mM) produces ROS in mouse liver microsomes in a concentration-dependent manner, with increased ROS generation at 0.3 mM and above^[1].

Parasitic Infection

Dicyclanil inhibits pupation of susceptible LS larvae with an IC₅₀ of 0.081 μg/assay, whereas Walgett 2019 G9-10 larvae exhibit 26-fold resistance with an IC₅₀ of 2.08 μg/assay^[8].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Anti-cancerOxidative StressCell Cycle RegulationInnate ImmunityLipid MetabolismParasitic Infection

Anti-cancer

Oxidative Stress

Dicyclanil (1,500 ppm; dietary oral administration; continuous dosing; 13 or 26 weeks) promotes GGT-positive foci and oxidative DNA damage in the livers of DMN-initiated, partially hepatectomized mice^[1].

Oxidative Stress

Dicyclanil (1,500 ppm; dietary oral administration; continuous dosing; 13 or 26 weeks) induces GGT-positive foci and oxidative DNA damage in the liver of non-initiated mice after 26 weeks^[1].

Oxidative Stress

Cell Cycle Regulation

Dicyclanil (DC) (0.15%; diet; ad libitum, daily; 13 weeks) increases hepatic 8-OHdG in both sexes and increases hepatocyte proliferation and gpt mutant frequency in female gpt delta mice, predominantly with GC:TA transversions^[3].

Oxidative Stress

Innate Immunity

Dicyclanil (1500 ppm; p.o.; continuous dosing; 7 weeks) causes mild lipofuscin deposition in the liver of male ICR mice and upregulates oxidative stress/DNA repair genes (including Gstm6), while activation of NF-kB increases slightly^[5].

Oxidative Stress

Lipid Metabolism

Dicyclanil (1500 ppm; p.o.; continuous dosing; 2 weeks) induces altered hepatic oxidative/metabolic gene expression and increases relative liver weight in healthy male ICR mice^[5].

Parasitic Infection

Anti-cancer

Dicyclanil (1,500 ppm; p.o.; continuous dosing; day 10 to the end of the study) combined with Clonorchis sinensis infection produces purpuric lesions of cystic mass formation and bile duct hyperplasia in C3H/He mice, but no cholangiocarcinoma^[4].
Dicyclanil (1,500 ppm; p.o.; continuous administration; day 10 to the end of the study) combined with Clonorchis sinensis and NDMA produces proliferative bile ducts, extensive fibrosis, and grayish-white nodules in C3H/He mice, but no cholangiocarcinoma^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

ICR (male, 5 weeks old at study start)^[1]
Dosage:

1,500 ppm in powdered diet
Administration:

oral; continuous; 13 or 26 weeks
Result:

Promoted GGT-positive foci and oxidative DNA damage in the livers of mice subjected to DMN initiation followed by partial hepatectomy.

Animal Model:

ICR (male, 5 weeks old at study start)^[1]
Dosage:

1,500 ppm in powdered diet
Administration:

oral; continuous; 13 or 26 weeks
Result:

Induced GGT-positive foci and oxidative DNA damage in the livers of uninitiated mice after 26 weeks.

Animal Model:

C3H/He (male, 4-5 weeks old, approximately 20 g)^[4]
Dosage:

1,500 ppm
Administration:

p.o. (drinking water); continuous; day 10 to end of study
Result:

One cystic mass-forming lesion was found in the caudate lobe, with a volume of 280.6 mm³; it was diagnosed as peliosis from dilated sinusoids.
The lesion center showed coagulative necrosis without inflammatory cells, and blood-filled cavities were present.
Bile duct dilatation and proliferation of ductal epithelial cells were observed in C. sinensis-infected groups.
Only 1 adult C. sinensis worm was recovered from a mouse.
No cholangiocarcinoma developed.

Animal Model:

C3H/He (male, 4-5 weeks old, approximately 20 g)^[4]
Dosage:

1,500 ppm
Administration:

p.o. (drinking water); continuous; day 10 to end of study
Result:

Gray-white nodule prevalence was 21.4%, and the highest mean intensity of gray-white nodules was 1.7.
Histopathology showed hyperproliferation of bile ducts with fibrotic changes and extensive fibrosis.
No cholangiocarcinoma developed.

Animal Model:

ICR (male, 5 weeks old at study start)^[5]
Dosage:

1500 ppm
Administration:

p.o.; continuous; weeks 1-8 (7 weeks)
Result:

Slight centrilobular hypertrophy of hepatocytes and diffuse small necrotic foci were observed.
Lipofuscin deposition was equivocal in 1/5, slight in 3/5, and absent in 1/5 mice.
Real-time RT-PCR showed up-regulation of Cyp1a1, Cyp1a2, Por, Ogg1, and Gstm6, down-regulation of Gadd45a, and a contradictory tendency for p53.
Activated NF-kB concentration showed a slight increase.

Related Protocols :

Chemical Information

CAS No. 112636-83-6
Appearance Solid
Molecular Weight 190.21
Formula C₈H₁₀N₆
Color White to off-white
SMILES

N#CC1=C(N)N=C(NC2CC2)N=C1N
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (328.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.2573 mL

26.2867 mL

52.5735 mL

5 mM

1.0515 mL

5.2573 mL

10.5147 mL

10 mM

0.5257 mL

2.6287 mL

5.2573 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (293 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Moto M, et al. Possible involvement of oxidative stress in dicyclanil-induced hepatocarcinogenesis in mice. Archives of toxicology. 2006 Oct;80(10):694-702.

[2]. Moto M, et al. Absence of in vivo genotoxicity and liver initiation activity of dicyclanil. The Journal of toxicological sciences. 2003 Aug;28(3):173-9.

[3]. Umemura T, et al. Detection of oxidative DNA damage, cell proliferation and in vivo mutagenicity induced by dicyclanil, a non-genotoxic carcinogen, using gpt delta mice. Mutation research. 2007 Sep 01;633(1):46-54.

[4]. Uddin MH, et al. C3H/He Mice as an Incompatible Cholangiocarcinoma Model by Clonorchis sinensis, Dicyclanil and N-Nitrosodimethylamine. The Korean journal of parasitology. 2016 Jun;54(3):281-9.

[5]. Moto M, et al. Molecular pathological analysis on the mechanism of liver carcinogenesis in dicyclanil-treated mice. Toxicology. 2005 Feb 28;207(3):419-36.

[6]. Jin M, et al. The threshold dose for liver tumor promoting effects of dicyclanil in ICR mice. The Journal of toxicological sciences. 2010 Feb;35(1):69-78.

[7]. Matsumoto S, et al. Suppressive effect of Siraitia grosvenorii extract on dicyclanil-promoted hepatocellular proliferative lesions in male mice. The Journal of toxicological sciences. 2009 Feb;34(1):109-18.

[8]. Kotze AC, et al. Resistance to dicyclanil and imidacloprid in the sheep blowfly, Lucilia cuprina, in Australia. Pest management science. 2022 Oct;78(10):4195-4206.

[9]. Cheesman MJ, et al. Soluble and membrane-bound Drosophila melanogaster CYP6G1 expressed in Escherichia coli: purification, activity, and binding properties toward multiple pesticides. Insect biochemistry and molecular biology. 2013 May;43(5):455-65.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.2573 mL	26.2867 mL	52.5735 mL	131.4337 mL
	5 mM	1.0515 mL	5.2573 mL	10.5147 mL	26.2867 mL
	10 mM	0.5257 mL	2.6287 mL	5.2573 mL	13.1434 mL
	15 mM	0.3505 mL	1.7524 mL	3.5049 mL	8.7622 mL
	20 mM	0.2629 mL	1.3143 mL	2.6287 mL	6.5717 mL
	25 mM	0.2103 mL	1.0515 mL	2.1029 mL	5.2573 mL
	30 mM	0.1752 mL	0.8762 mL	1.7524 mL	4.3811 mL
	40 mM	0.1314 mL	0.6572 mL	1.3143 mL	3.2858 mL
	50 mM	0.1051 mL	0.5257 mL	1.0515 mL	2.6287 mL
	60 mM	0.0876 mL	0.4381 mL	0.8762 mL	2.1906 mL
	80 mM	0.0657 mL	0.3286 mL	0.6572 mL	1.6429 mL
	100 mM	0.0526 mL	0.2629 mL	0.5257 mL	1.3143 mL

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Dicyclanil

All DNA/RNA Synthesis Isoforms

All Cytochrome P450 Isoforms